Adiponectin protects the kidney against chronic intermittent hypoxia-induced injury through inhibiting endoplasmic reticulum stress

Abstract

Resveratrol, a polyphenol compound derived from various edible plants, protects against sepsis-induced acute kidney injury (AKI) via its anti-inflammatory activity, but the underlying mechanisms remain largely unknown. In this study, a rat model of sepsis was established by cecal ligation and puncture (CLP), 30 mg/kg resveratrol was intraperitoneally administrated immediately after the CLP operation. HK-2 cells treated by 1 μg/ml lipopolysaccharide, 0.2 μM tunicamycin, 2.5 mM irestatin 9389 and 20 μM resveratrol were used for in vitro study. The results demonstrated that resveratrol significantly improved the renal function and tubular epithelial cell injury and enhanced the survival rate of CLP-induced rat model of sepsis, which was accompanied by a substantial decrease of the serum content and renal mRNA expressions of TNF-α, IL-1β and IL-6. In addition, resveratrol obviously relieved the endoplasmic reticulum stress, inhibited the phosphorylation of inositol-requiring enzyme 1(IRE1) and nuclear factor-κB (NF-κB) in the kidney. In vitro studies showed that resveratrol enhanced the cell viability, reduced the phosphorylation of NF-κB and production of inflammatory factors in lipopolysaccharide and tunicamycin-induced HK-2 cells through inhibiting IRE1 activation. Taken together, administration of resveratrol as soon as possible after the onset of sepsis could protect against septic AKI mainly through inhibiting IRE1-NF-κB pathway-triggered inflammatory response in the kidney. Resveratrol might be a readily translatable option to improve the prognosis of sepsis.

Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis

“…In comparison, our study found that the level of oxidative stress increased in the kidney tissue in the CIH group. This finding is also in accordance with other reports that CIH caused the production of ROS (27 –29). Wysocki et al.…”

A critical role for adiponectin‐mediated development of endometrial luminal epithelial cells during the peri‐implantation period of pregnancy

“…Then, the tunicamycin‐induced increase in expression of UPR signaling molecules by ER stress was decreased by adiponectin in pLE cells. Results of previous studies also indicate inhibitory effects of adiponectin on ER stress in adipocytes (Liu et al, ), and cells of the liver (Jung et al, ), kidney (Ding et al, ), heart (Guo et al, ), and smooth muscle (Lu et al, ). These results indicate that adiponectin may prevent ER stress in uterine LE during early pregnancy.…”

Inhibition of Epidermal Growth Factor Receptor Activation Is Associated With Improved Diabetic Nephropathy and Insulin Resistance in Type 2 Diabetes

“…Preservation of circulating adiponectin levels should be considered among the mechanism(s) contributing to the kidney-protective effect seen with EGFR inhibition. Adiponectin has been shown to be renoprotective in different kidney injury models and after podocyte injury (38,39). It protects against hypoxia-induced kidney injury by inhibiting endoplasmic reticulum stress (40).…”