Adipose-derived cellular and cell-derived regenerative therapies in dermatology and aesthetic rejuvenation

Suh, Annie; Pham, Anthony; Cress, Mikel Jean; Pincelli, Thais; TerKonda, Sarvam P.; Bruce, Alison J.; Zubair, Abba C.; Wolfram, Joy; Shapiro, Shane A.

doi:10.1016/j.arr.2019.100933

Cited by 79 publications

(67 citation statements)

References 108 publications

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“…Specifically, qEV columns (Izon Science) were used to separate EVs from Lipo-NPs. Briefly, the column was equilibrated with sucrose buffer, Lipo-NPs (200 µL) were loaded into the column, and five EV-enriched fractions (7)(8)(9)(10)(11) were collected for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Tian

Ticer

Wang

et al. 2020

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Extracellular vesicles secreted from adipose‐derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti‐inflammatory properties of adipose tissue‐derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC‐EVs, lipoaspirate nanoparticles (Lipo‐NPs) take less time to process (hours compared to months) and cost less to produce (clinical‐grade cell culture facilities are not required). The physicochemical characteristics and anti‐inflammatory properties of Lipo‐NPs are evaluated and compared to those of patient‐matched ADSC‐EVs. Moreover, guanabenz loading in Lipo‐NPs is evaluated for enhanced anti‐inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo‐NPs compared to ADSC‐EVs. Additionally, the uptake of Lipo‐NPs in hepatocytes and macrophages is higher. Lipo‐NPs and ADSC‐EVs have comparable protective and anti‐inflammatory effects. Specifically, Lipo‐NPs reduce toll‐like receptor 4‐induced secretion of inflammatory cytokines in macrophages. Guanabenz‐loaded Lipo‐NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.

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Section: Methodsmentioning

confidence: 99%

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Tian

Ticer

Wang

et al. 2020

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“…According to different sources, the research on hair regeneration can be divided into three directions: Adipose-derived, blood-derived, and HF-derived. For adipose-derived research [44][45][46], the components that are typically used for aesthetic and dermal applications consist of nanofat, stromal vascular fraction cells (SVFs), adipose-derived mesenchymal stem cells (AD-MSCs), and extracellular vesicle (EVs), which have all shown capability to repair, regenerate, and rejuvenate surrounding tissue. For blood-derived research [47][48][49][50][51], studies focused on platelet-rich plasma (PRP), which is a whole-blood centrifuged concentrate that contains proteins and growth factors (GFs) but not red blood cells.…”

Section: Discussionmentioning

confidence: 99%

Thymosin β4 Identified by Transcriptomic Analysis from HF Anagen to Telogen Promotes Proliferation of SHF-DPCs in Albas Cashmere Goat

Dai¹,

Fei²,

Xu³

et al. 2020

IJMS

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Increasing cashmere yield is one of the important goals of cashmere goat breeding. To achieve this goal, we screened the key genes that can improve cashmere performance. In this study, we used the RNA raw datasets of the skin and dermal papilla cells of secondary hair follicle (SHF-DPCs) samples of hair follicle (HF) anagen and telogen of Albas cashmere goats and identified a set of significant differentially expressed genes (DEGs). To explore potential associations between gene sets and SHF growth features and to identify candidate genes, we detected functional enrichment and constructed protein-protein interaction (PPI) networks. Through comprehensive analysis, we selected Thymosin β4 (Tβ4), Rho GTPase activating protein 6 (ARHGAP6), ADAM metallopeptidase with thrombospondin type 1 motif 15, (ADAMTS15), Chordin (CHRD), and SPARC (Osteonectin), cwcv and kazal-like domains proteoglycan 1 (SPOCK1) as candidate genes. Gene set enrichment analysis (GSEA) for these genes revealed Tβ4 and ARHGAP6 have a close association with the growth and development of SHF-DPCs. However, the expression of Tβ4 in the anagen was higher than that in the telogen, so we finally chose Tβ4 as the ultimate research object. Overexpressing Tβ4 promoted and silencing Tβ4 inhibited the proliferation of SHF-DPCs. These findings suggest that Tβ4 can promote the growth and development of SHF-DPCs and indicate that this molecule may be a valuable target for increasing cashmere production.

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“…The use of the fat grafting technique is finding an even wider field in regenerative medicine. In fact, it has proved valid in a wide spectrum of pathological situations ranging from the treatment of scars to the healing of ulcers and numerous other conditions that are reported in excellent reviews (Bellini et al, 2017;Caruana et al, 2015;Charlesde-Sá et al, 2015;Frese et al, 2016;Gal et al, 2019;Pu et al, 2015;Rigotti et al, 2007;Simonacci et al, 2017;Suh et al, 2019;Tremolada et al, 2016;Trivisonno et al, 2019;Uyulmaz et al, 2018). It should be noted that today, fat grafting is also used in situations unthinkable in the past, such as the use in articular diseases (Bansal et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…. Fat grafting has therefore become a procedure of reconstructive, aesthetic, and regenerative medicine (Bellini et al, 2017;Caruana et al, 2015;Charles-de-Sá et al, 2015;Frese et al, 2016;Gal et al, 2019;Rigotti et al, 2007;Simonacci et al, 2017;Suh et al, 2019;Tremolada et al, 2016;Trivisonno et al, 2019;Uyulmaz et al, 2018). The potential in the latter field is undoubtedly remarkable, and the applications are multiplying.…”

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confidence: 99%

Comparative technical analysis of lipoaspirate mechanical processing devices

Veronese

Prè

Conti

et al. 2020

J Tissue Eng Regen Med

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Fat grafting is a well‐established procedure in reconstructive, aesthetic, and regenerative medicine, in particular due to the presence in the adipose tissue of a high concentration of mesenchymal stem cells. The need to reduce fat processing times, for an immediate clinical use and regulatory restrictions on the degree of manipulation of human tissues, has led to the development of numerous devices for the mechanical, nonenzymatic processing of adipose tissue. The aim of this study is to describe the state of the art of mechanical devices used for fat processing, performing a technical analysis of the currently commercially available devices. This should facilitate the development of new devices that improve therapeutic results.

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Adipose-derived cellular and cell-derived regenerative therapies in dermatology and aesthetic rejuvenation

Cited by 79 publications

References 108 publications

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

Thymosin β4 Identified by Transcriptomic Analysis from HF Anagen to Telogen Promotes Proliferation of SHF-DPCs in Albas Cashmere Goat

Comparative technical analysis of lipoaspirate mechanical processing devices

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