Adipose derived mesenchymal stem cells transplantation via portal vein improves microcirculation and ameliorates liver fibrosis induced by CCl4 in rats

Wang, Yu; Fan, Li; Li, Jiaping; Fan, Wenzhe; Xu, Hanshi; Yang, Xia; Liang, Liuqin; Chen, Wei; Yang, Jun

doi:10.1186/1479-5876-10-133

Cited by 86 publications

(66 citation statements)

References 48 publications

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“…ADRCs have been reported to inhibit fibrosis of the liver [15], but there has been no previous report of fibrosis being caused by the grafting of large numbers of these cells. This study demonstrates that the concentration of transplanted ADRCs affects the engraftment and quality of the grafted fat.…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived regenerative cell (ADRC)-enriched fat grafting: optimal cell concentration and effects on grafted fat characteristics

et al. 2013

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BackgroundTo overcome the absorption of traditional fat grafting, techniques for adipose-derived regenerative cell (ADRC)-enriched fat grafting are currently being adapted for practical application. The Celution®800/CRS (Cytori Therapeutics, San Diego, CA) has enabled rapid grafting of the patient’s own freshly harvested ADRCs without requiring a culturing step. However, the optimal cell concentration and the effects of ADRCs on the characteristics of grafted fat after free fat grafting remain unclear.MethodsADRCs were isolated and purified from human fat tissue using the Celution®800/CRS. Animals that received fat grafting without the addition of ADRCs were designated the control group (group A). The number of ADRCs per grafted fat volume (mL) was adjusted to 3 × 105, 1.5 × 106, and 3 × 106 cells/mL (groups B, C, and D, respectively), mixed with free fat, and transplanted as ADRC-enriched fat grafting. These mixtures were transplanted subcutaneously into BALB/C Jcl-nu/nu mice. The volume of grafted fat was determined 5 months after transplantation, and histological assessments were performed.ResultsADRC-enriched fat grafting resulted in decreased fat absorption and the formation of greater numbers of new blood vessels in the grafted fat. The optimal ADRC concentration in this study was found to be 3 × 105 cells/mL (group B), with higher concentrations resulting in increased cyst and fibril formation in the grafted fat.ConclusionsThis study used the Celution®800/CRS for free fat grafting and demonstrated that the concentration of transplanted ADRCs affected the engraftment and quality of the grafted fat.

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Section: Discussionmentioning

confidence: 99%

Adipose-derived regenerative cell (ADRC)-enriched fat grafting: optimal cell concentration and effects on grafted fat characteristics

et al. 2013

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“…Mesenchymal stem cells (MSCs) reside in various tissues, such as bone marrow, umbilical cord blood, placenta, liver, adipose tissue and others. MSCs undergo self-renewal but can differentiate into multiple cell lineages; and have demonstrated immunomodulation, inflammation suppression and antifibrogenic effects [1–5]. MSCs isolated from adipose tissue are called adipose derived stromal cells (ADSCs).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Administration of ADSCs-Based Therapy and Gene Therapy Using Ad-huPA Reduces Experimental Liver Fibrosis

et al. 2016

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Background and AimshADSCs transplantation in cirrhosis models improves liver function and reduces fibrosis. In addition, Ad-huPA gene therapy diminished fibrosis and increased hepatocyte regeneration. In this study, we evaluate the combination of these therapies in an advanced liver fibrosis experimental model.MethodshADSCs were expanded and characterized before transplantation. Ad-huPA was simultaneously administrated via the ileac vein. Animals were immunosuppressed by CsA 24 h before treatment and until sacrifice at 10 days post-treatment. huPA liver expression and hADSCs biodistribution were evaluated, as well as the percentage of fibrotic tissue, hepatic mRNA levels of Col-αI, TGF-β1, CTGF, α-SMA, PAI-I, MMP2 and serum levels of ALT, AST and albumin.ResultshADSCs homed mainly in liver, whereas huPA expression was similar in Ad-huPA and hADSCs/Ad-huPA groups. hADSCs, Ad-huPA and hADSCs/Ad-huPA treatment improves albumin levels, reduces liver fibrosis and diminishes Collagen α1, CTGF and α-SMA mRNA liver levels. ALT and AST serum levels showed a significant decrease exclusively in the hADSCs group.ConclusionsThese results showed that combinatorial effect of cell and gene-therapy does not improve the antifibrogenic effects of individual treatments, whereas hADSCs transplantation seems to reduce liver fibrosis in a greater proportion.

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“…ADSCs exhibit plasticity, cell surface markers, and a differentiation capacity similar to bone marrow-derived MSCs (Bonora-Centelles et al 2009;Baer 2011;Harn et al 2012). According to previous reports, ADSCs not only differentiate into hepatocyte-like cells (Al Battah et al 2011;Abbas Sahebghadam 2012;Wang et al 2012) but can also restore liver function in animal models (Mohsin et al 2011;Harn et al 2012;Saito et al 2014). Several preclinical studies have shown the efficiency of ADSCs in the treatment of liver disease (Wang et al 2012;Koellensperger et al 2013).…”

Section: Introductionmentioning

confidence: 90%

A comparison of the chemical and liver extract-induced hepatic differentiation of adipose derived stem cells

Nhung

Nam

Nguyen

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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Adipose-derived stem cells (ADSCs) have been put forward as promising therapeutics for end-stage liver disease (ESLD). In the present study, we compared the effects of defined chemicals and liver extract on the hepatic differentiation of ADSCs. ADSCs were isolated according to the method described in our previously published study. Subsequently, the differentiation of ADSCs was induced separately by chemicals (including hepatic growth factor (HGF), fibroblast growth factor (FGF), and oncostatin M (OSM)) and liver extract (30 μg/ml) in a total period of 21 d. The efficiency of hepatic differentiation was evaluated by changes in the cell morphology, gene expression, and cellular function. The results showed that the liver extract promoted the hepatic differentiation of ADSCs to a significantly greater extent than the chemicals. In the group of ADSCs treated with liver extract, changes in the cell morphology began sooner, and the expression of alpha-FP and albumin genes was higher than that in the chemically treated group. The ADSCs in both the groups stained positive for anti-alpha trypsin (AAT) and albumin markers. The cells also exhibited glycogen storage capacity. Therefore, we concluded that the liver extract could efficiently induce the differentiation of ADSCs into hepatocyte-like cells. This study reveals the potential of mesenchymal stem cell differentiation in the liver extract, which supports further preclinical and clinical research on the application of ADSCs in ESLD treatment.

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Adipose derived mesenchymal stem cells transplantation via portal vein improves microcirculation and ameliorates liver fibrosis induced by CCl4 in rats

Cited by 86 publications

References 48 publications

Adipose-derived regenerative cell (ADRC)-enriched fat grafting: optimal cell concentration and effects on grafted fat characteristics

Adipose-derived regenerative cell (ADRC)-enriched fat grafting: optimal cell concentration and effects on grafted fat characteristics

Simultaneous Administration of ADSCs-Based Therapy and Gene Therapy Using Ad-huPA Reduces Experimental Liver Fibrosis

A comparison of the chemical and liver extract-induced hepatic differentiation of adipose derived stem cells

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