Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats

Suartz, Caio Vinicius; Gaiba, Silvana; França, Jerônimo Pereira de; Aloise, Antônio Carlos; Ferreira, Lydia Masako

doi:10.1590/s0102-86502014001400002

Cited by 10 publications

(8 citation statements)

References 26 publications

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“…However, the limiting factor for the use of skin flaps is the unpredictability of its distal vascularization, which can lead to irreversible microcirculation, followed by partial or complete necrosis of the flaps and making wound more susceptible to infection. Therefore, improving the viability of random flaps, especially in its distal end, has been an important goal for the success of this technique 1 .…”

Section: Introductionmentioning

confidence: 99%

Action of hyperbaric oxygenation in the rat skin flap

et al. 2015

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PURPOSE:To evaluate the morphology, necrotic area and collagen content in skin flaps of rats subjected to hyperbaric oxygenation (HBO). METHODS:Forty adult rats were divided into four groups: GEC -epilated; GE/HBO -epilated subjected to HBO; GER -epilated submitted to skin flap (2 cm in width /8 cm length in the dorsal area) and GER/HBO -epilated, subjected to skin flap and HBO. HBO (2.4 ATA) was performed for two hours during seven consecutive days. In the eighth day, the rats were anesthetized and the skin flaps were removed and separated into three portions, relative to pedicle fixation. The material fixed in 10% formalin was processed for paraffin embedding; sections were stained by H.E and subjected to picrosirius-red method. The slides examined under light microscopy for evaluation of the collagen content in polarized light microscope and ImageLab ® software (Bio-Rad). RESULTS:The data showed larger area of necrosis and lower levels of collagen in the three regions of the GER group, whereas in the GER/HBO group the collagen content was similar to the GEC and GE/HBO groups. CONCLUSION:Hyperbaric oxygenation reduced the area of necrosis and preserved the morphology and collagen content in skin flaps of rats.

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Section: Introductionmentioning

confidence: 99%

Action of hyperbaric oxygenation in the rat skin flap

et al. 2015

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“…Adipose-derived stem cells is already used for healing of oncology lesions from radiotherapy in humans, ameliorate skin flap viability and chronic skin ulcers with diabetic mice 8,11,14,15 . The focus of this study was to evaluate in acute wounds.…”

Section: Resultsmentioning

confidence: 99%

Stem cells from adipose tissue improve the time of wound healing in rats

et al. 2016

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PURPOSE:To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS:Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS:All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions.CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.

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“…In this step, the adult male rats weighing (250-300 g) were anesthetized under ketamine (Rotex Medica) and Xylazine (Alfasan) anaesthetic combinations (60 mg/kg I.P, and 10 mg/kg I.P, respectively). 49 Osteoarthritis was induced by intra-articular injection of 20μl of collagenase II(Gibco, USA) (90 mg/ml) into the knee joint of rats through the patellar ligament with the use of an insulin syringe. 50 , 51 To provide pain relief oral dose‏ of 300 mg/kg of acetaminophen (Rouz Darou) dissolved in drink water was administered for rats.…”

Section: Methodsmentioning

confidence: 99%

An Investigation on the Regenerative Effects of Intra Articular Injection of Co-Cultured Adipose Derived Stem Cells with Chondron for Treatment of Induced Osteoarthritis

Jacer

Shafaei

2018

Adv Pharm Bull

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Purpose: Adipose tissue derived stem cells (ASCs) and chondrocytes are best cells for articular cartilage regeneration. Chondrocyte with peri-cellular matrix (PCM) is called chondron provides ideal microenviroment than chondrocytes. We aimed to evaluate the regenerative effects of intra-articular injection of ASCs co-cultures with chondron in induced osteoarthritis (OA).Methods: ASC, from the peri-renal fat of male rat and chondron from primary newborn rat hyaline cartilage were isolated. ASCs were cultured for at least three passages in vitro. Six weeks after OA induction, rats were randomly distributed in five groups of control, osteoarthritic, ASC, chondron and co-cultured. ASCs (107), chondrons (107) and combination of chondrons and ASCs (107) were injected into intra-articular space of the rat knee. The effect of treatments was evaluated by macroscopic and microscopic examinations. The expression levels of collagen type ΙΙ was studied by immunohistochemistry.Results: Macroscopic appearance of the co-cultured group, showed much enhanced articular cartilage regeneration compared to ASC and chondron groups. H&E showed evidence of repair site of articular surface without erosion and fibrillation versus OA group which showed thin layer of hyaline cartilage over tidemark and spontaneous fibrocartilage formation. Metachromatic regions stained with toluidine blue were larger in treatment groups versus OA group. Strong intensity of type ΙΙ collagen staining was observed in co-culture group compared to other groups.Conclusion: Co-culture of chondrons and ASCs increased articular hyaline cartilage formation and provides a useful tool to improve limitations of each of applied cells in this model.

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Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats

Cited by 10 publications

References 26 publications

Action of hyperbaric oxygenation in the rat skin flap

Action of hyperbaric oxygenation in the rat skin flap

Stem cells from adipose tissue improve the time of wound healing in rats

An Investigation on the Regenerative Effects of Intra Articular Injection of Co-Cultured Adipose Derived Stem Cells with Chondron for Treatment of Induced Osteoarthritis

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