Adipose‐Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications

Abstract

Adipose-derived stem cells (ASCs) are a mesenchymal stem cell source with properties of self-renewal and multipotential differentiation. Compared to bone marrow-derived stem cells (BMSCs), ASCs can be derived from more sources and are harvested more easily. Three-dimensional (3D) tissue engineering scaffolds are better able to mimic the in vivo cellular microenvironment, which benefits the localization, attachment, proliferation, and differentiation of ASCs. Therefore, tissue-engineered ASCs are recognized as an attractive substitute for tissue and organ transplantation. In this paper, we review the characteristics of ASCs, as well as the biomaterials and tissue engineering methods used to proliferate and differentiate ASCs in a 3D environment. Clinical applications of tissue-engineered ASCs are also discussed to reveal the potential and feasibility of using tissue-engineered ASCs in regenerative medicine.

Limbal epithelial stem cells in regeneration of corneal epithelium – clinical and molecular aspects

“…Zależnie od pochodzenia komórki te można podzielić na grupy: embrionalne komórki macierzyste (embryonic stem cells -ESC), somatyczne komórki macierzyste (adult stem cells -ASC), do których należą m.in. mezenchymalne komórki macierzyste oraz indukowane pluripotentne komórki macierzyste (induced pluripotent stem cellsiPSC) [3,4]. Somatyczne komórki macierzyste zwykle są multipotentne, co oznacza, że różnicują się tylko do jednego, tego samego listka zarodkowego, z którego pochodzą [2].…”

Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

“…In recent years, ASCs have been under extensive study in regenerative medicine 15, 16, 17, 18. They are easily retrieved in high numbers from fat tissue and expanded in vitro, which makes them an attractive tool in tissue reconstruction 14, 19, 20.…”

Protective Role of Stem Cell Derived Extracellular Vesicles in an In Vitro Model of Hyperglycemia-Induced Endothelial Injury

“…Adipose-derived stem cells (ASC) are mesenchymal stem cells with self-renewal property and multipotent differentiation, such as adipogenesis, osteogenesis, and chondrogenesis. Like MSC, ASC showed a promising regenerative potential in various diseases, but they became more attractive for therapeutic purposes because they can be easily isolated from subcutaneous adipose tissue [23]. Recent evidence shows that EVs derived from ASC (ASC EV) contain several angiogenic factors, such as Milk fat globule-EGF factor 8 protein (MFGE8), angiopoietin like 1 (ANGPTL1), and thrombopoietin [24].…”