Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

Xiao, Dongdong; Wang, Qiong; Hu, Yan; Lv, Xiangguo; Yang, Zhao; Zhou, Zhe; Zhang, Ming; Sun, Qian; Sun, Kang; Li, Wei; Lu, Mengji

doi:10.18632/oncotarget.21211

Cited by 12 publications

(9 citation statements)

References 44 publications

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“…Despite the functional and structural regeneration of the bioengineered neobladders described in several preclinical studies in rodents [ 87 , 136 ], limited and so far non efficient clinical translation has been reported. First, porous matrices composed of a diverse range of biomaterials alone or forming a composite of natural and synthetic nature were used for urinary bladder regeneration, with a wide range of outcomes in several clinical trials, and failed to meet their expectations in most cases (reviewed in [ 134 ]).…”

Section: Tissue Engineering For Urinary Bladder Regenerationmentioning

confidence: 99%

Bioengineering Approaches for Bladder Regeneration

Serrano‐Aroca

Donoso

Moreno‐Manzano

2018

IJMS

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Current clinical strategies for bladder reconstruction or substitution are associated to serious problems. Therefore, new alternative approaches are becoming more and more necessary. The purpose of this work is to review the state of the art of the current bioengineering advances and obstacles reported in bladder regeneration. Tissue bladder engineering requires an ideal engineered bladder scaffold composed of a biocompatible material suitable to sustain the mechanical forces necessary for bladder filling and emptying. In addition, an engineered bladder needs to reconstruct a compliant muscular wall and a highly specialized urothelium, well-orchestrated under control of autonomic and sensory innervations. Bioreactors play a very important role allowing cell growth and specialization into a tissue-engineered vascular construct within a physiological environment. Bioprinting technology is rapidly progressing, achieving the generation of custom-made structural supports using an increasing number of different polymers as ink with a high capacity of reproducibility. Although many promising results have been achieved, few of them have been tested with clinical success. This lack of satisfactory applications is a good reason to discourage researchers in this field and explains, somehow, the limited high-impact scientific production in this area during the last decade, emphasizing that still much more progress is required before bioengineered bladders become a commonplace in the clinical setting.

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Section: Tissue Engineering For Urinary Bladder Regenerationmentioning

confidence: 99%

Bioengineering Approaches for Bladder Regeneration

Serrano‐Aroca

Donoso

Moreno‐Manzano

2018

IJMS

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“…ASCs are considered as the most suitable source of cells for stem cell-based therapies mainly because they can be harvested in large quantities using minimally invasive procedures 19 – 21 . It has been shown that ASCs secrete a wide variety of soluble mediators that promote morphological regeneration and functional restoration of bladder defects 22 – 24 . Possible triggering of cancer recurrence during remission remains, however, a significant concern in the application of stem cell-based therapies for cancer patients.…”

Section: Introductionmentioning

confidence: 99%

The interplay between adipose-derived stem cells and bladder cancer cells

Maj

Kokocha

Bajek

et al. 2018

Sci Rep

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Tissue engineering approaches offer alternative strategies for urinary diversion after radical cystectomy. Possible triggering of cancer recurrence remains, however, a significant concern in the application of stem-cell based therapies for oncological patients. Soluble mediators secreted by stem cells induce tissue remodelling effects, but may also promote cancer cells growth and metastasis. We observed a substantial increase in the concentration of IL-6 and IL-8 in the secretome of adipose-derived stem cells (ASCs) co-cultured with bladder cancer cells. Concentrations of GM-CSF, MCP-1 and RANTES were also elevated. Bioactive molecules produced by ASCs increased the viability of 5637 and HT-1376 cells by respectively 15.4% and 10.4% (p < 0.0001). A trend in reduction of adhesion to ECM components was also noted, even though no differences in β-catenin expression were detected. When HT-1376 cells were co-cultured with ASCs their migration and invasion increased by 24.5% (p < 0.0002) and 18.2% (p < 0.002). Expression of p-ERK1/2 increased in 5637 cells (2.2-fold; p < 0.001) and p-AKT in HB-CLS-1 cells (2.0-fold; p < 0.001). Our results confirm that ASCs crosstalk with bladder cancer cells in vitro what influences their proliferation and invasive properties. Since ASCs tropism to tumour microenvironment is well documented their application towards post-oncologic reconstruction should be approached with caution.

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“…To track SPIO-labeled NSCs in vivo, we chose early-passage NSCs (P2 or P3) and the SPIO labeling time of 12 hours. Early-passage NSCs were chosen over late-passage NSCs because they were exposed to the in vitro stress condition for a shorter period of time and hence might better represent cells in vivo 36, 37 . A 12-hour labeling time was also preferred to a 48- or 72-hour labeling time for the same reason, especially if it showed the same labeling efficiency as did the 48- or 72-hour labeling.…”

Section: Resultsmentioning

confidence: 99%

Grafted neural stem cells show lesion-specific migration in radiation-injured rat brains

Bai

Wang

et al. 2018

RSC Adv.

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Neural stem cells (NSCs) exhibit preferential homing toward some types of brain lesion, but their migratory property during radiation brain injury (RBI) remains unexplored. Here, we use the superparamagnetic iron oxide (SPIO)-labeled magnetic resonance imaging (MRI) technology to determine the migration of transplanted NSCs in two partial RBI models in real time created by administering 30-55 Gy of radiation to the right or posterior half of the adult rat brain. SPIO-labeled NSCs were stereotactically grafted into the uninjured side one week after RBI. The migration of SPIO-labeled NSCs in live radiation-injured brains was traced by MRI for up to 28 days after engraftment and quantified for their moving distances and speeds. A high labeling efficiency (>90%) was achieved by incubating NSCs with 100μg/ml of SPIO for 12-24 hours. Upon stereotactic transplantation into the healthy side of the brain, SPIO-labeled NSCs were distinctively detected as hypointense signals on T2-weighted images (T2WI), showed sustained survival for up to 4 weeks, and exhibited directional migration to the radiation-injured side of the brain with a speed of 86-127 μm/day. The moving kinetics of grafted NSCs displayed no difference in brains receiving a high (55 Gy) vs. moderate (45 Gy) dose of radiation, but was slower in the right RBI model than in the posterior RBI model. This study shows that NSCs can be effectively labeled by SPIO and traced in vivo by MRI, and that grafted NSCs exhibit directional migration toward RBI sites in a route-dependent but radiation dose-independent manner.

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Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

Cited by 12 publications

References 44 publications

Bioengineering Approaches for Bladder Regeneration

Bioengineering Approaches for Bladder Regeneration

The interplay between adipose-derived stem cells and bladder cancer cells

Grafted neural stem cells show lesion-specific migration in radiation-injured rat brains

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