Adipose-derived stromal/stem cells

Gimble, Jeffrey M.; Bunnell, Bruce A.; Frazier, Trivia; Rowan, Brian G.; Shah, Forum; Thomas-Porch, Caasy; Wu, Xiying

doi:10.4161/org.24279

Cited by 100 publications

(92 citation statements)

References 69 publications

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“…2,16 As an essential micronutrient, vitamin C is often added to culture medium for cell growth. In the present study, we found that the supplementation of vitamin C at an optimal concentration in culture medium was beneficial to the proliferation of human ADSCs by regulating the p53-p21 pathway and related cell cycle progression.…”

Section: Discussionmentioning

confidence: 99%

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Zhang

et al. 2016

Organogenesis

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ABSTRACT. Although adipose-derived stem cells (ADSCs) have demonstrated a promising potential for the applications of cell-based therapy and regenerative medicine, excessive reactive oxygen species (ROS) are harmful to ADSCs cell survival and proliferation. Vitamin C is an important antioxidant, and is often added into culture media as an essential micronutrient. However, its roles on the proliferation of human ADSCs have not been studied. Therefore, in this study, human ADSCs were isolated, and detected by flow cytometry for the analysis of their cell surface antigens. Cell proliferation and cell cycle progression were measured with cell counting kit-8 assay and flow cytometry, respectively. Western blotting was used to detect the expression levels of cyclin E1, p53, p21, and CDK2 proteins. The effect of vitamin C pretreatment on the production of hydrogen peroxide (H 2 O 2 )-mediated ROS in the ADSCs was evaluated by flow cytometry. Our results indicated that vitamin C treatment significantly increased cell proliferation, and changed the cell cycle distribution of ADSCs by decreasing the percentage of G 1 phase, and concurrently increased the percentage of S and G 2 /M phase. Western blot analysis indicated that vitamin C treatment up-regulated the expression levels of cyclin E1 and CDK2, but down-regulated p53 and p21 proteins expression, which contributed to cell proliferation and cell cycle progression. Vitamin C pretreatment significantly reduced the production of H 2 O 2 -induced ROS in the ADSCs. These findings suggest that vitamin C can promote the proliferation and cell cycle progression in the ADSCs possibly through regulation of p53-p21 signal pathway.

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Section: Discussionmentioning

confidence: 99%

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Zhang

et al. 2016

Organogenesis

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“…It is known that ADSCs secrete numerous cytokines and growth factors such as TGF, FGF and TNF and that the efficacy of cell therapy with ASCs is related to their paracrine effects (39,40). Therefore it is possible that while in the cusps, the ADSCs have secreted pro-fibrotic agents which stimulated and accelerated the fibrous tissue overgrowth.…”

Section: Cell-seeded Valves and In Vivo Performancementioning

confidence: 99%

Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells

Harpa

Movileanu

Sierad³

et al. 2015

Revista Romana De Medicina De Laborator

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“…Thus, the therapeutic efficacy of BM-MSCs transplantation remains controversial at present [21]. Adipose tissue-derived progenitor/ stem cells are an attractive cell source for cell therapy for liver fibrosis, based on several properties of these cells; 1) ample production of fibrinolytic enzymes and cytokines [22], 2) ease of obtaining stem cells compared to other tissue-specific stem cells including BM-MSCs, [23]. The use of adipose tissue-derived multi-lineage progenitor cells (ADMPCs) supports the view that fibrinolytic enzyme-secretion could mediate the therapeutic mode of actions in liver fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of adipose tissue-derived multi-lineage progenitor cell-sheets on liver fibrosis

Okura¹,

Morita²,

Fujita³

et al. 2017

J Transl Sci

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Adipose-derived stromal/stem cells

Cited by 100 publications

References 69 publications

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells

Therapeutic potential of adipose tissue-derived multi-lineage progenitor cell-sheets on liver fibrosis

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