Adipose mesenchymal stem cells in the field of bone tissue engineering

Romagnoli, Cecilia; Brandi, Maria Luisa

doi:10.4252/wjsc.v6.i2.144

Cited by 79 publications

(72 citation statements)

References 104 publications

(115 reference statements)

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“…For example, adipose mesenchymal stem cells are promising due to their large quantities and the procedure for obtaining adipose MSCs is relatively noninvasive and causes little discomfort. 73,109 In addition, the in vivo microenvironment can be optimally mimicked by incorporating, co-culture into research projects. Co-culture techniques play an important role in enabling complex cellcell interaction, allowing better elucidation of in vivo activities.…”

Section: Perspectivementioning

confidence: 99%

Three dimensionalde novomicro bone marrow and its versatile application in drug screening and regenerative medicine

Liu

et al. 2015

Exp Biol Med (Maywood)

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The finding that bone marrow hosts several types of multipotent stem cell has prompted extensive research aimed at regenerating organs and building models to elucidate the mechanisms of diseases. Conventional research depends on the use of two-dimensional (2D) bone marrow systems, which imposes several obstacles. The development of 3D bone marrow systems with appropriate molecules and materials however, is now showing promising results. In this review, we discuss the advantages of 3D bone marrow systems over 2D systems and then point out various factors that can enhance the 3D systems. The intensive research on 3D bone marrow systems has revealed multiple important clinical applications including disease modeling, drug screening, regenerative medicine, etc. We also discuss some possible future directions in the 3D bone marrow research field.

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Section: Perspectivementioning

confidence: 99%

Three dimensionalde novomicro bone marrow and its versatile application in drug screening and regenerative medicine

Liu

et al. 2015

Exp Biol Med (Maywood)

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“…In cell regeneration, ADMSCs are capable of multilineage differentiation, and show several advantages such as their abundant supply, high yield, easy accessibility, and non-immunogenic property (Romagnoli and Brandi, 2014). However, their clinical application remains limited.…”

Section: Discussionmentioning

confidence: 99%

hBMP-7 induces the differentiation of adipose-derived mesenchymal stem cells into osteoblast-like cells

et al. 2016

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ABSTRACT. The aim of this study was to investigate the differentiation potential of adipose-derived mesenchymal stem cells (ADMSCs) into osteoblasts by human bone morphogenetic protein-7 (hBMP-7) induction. ADMSCs were isolated from the subcutaneous adipose tissue of a rabbit, and then transfected with the pcDNA3.1 vector alone and pcDNA3.1-hBMP-7 (hBMP-7), respectively. Untransfected ADMSCs were used as the control group. After transfection, the morphology and green fluorescent protein (GFP) fluorescence intensity of ADMSCs were observed by fluorescent microscopy. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to detect the growth of ADMSCs at 1, 3, and 5 days, respectively. Transmission electron microscopy was performed to observe the ultrastructural morphology of ADMSCs. In addition, ADMSCs were stained with quinalizarin and toluidine blue to reflect the content of osteoblasts and chondrocytes, respectively. Finally, the expression of collagen I and osteocalcin in ADMSCs was detected by western blot. ADMSCs were successfully isolated. Obvious GFP fluorescence and high expression of hBMP-7 demonstrated the successful transfection of hBMP-7. Specific morphological characters with a metabolically active ultrastructure were exhibited on the ADMSCs transfected with hBMP-7. In addition, the growth rate of ADMSCs transfected with hBMP-7 was significantly higher than that of the cells in the vector and control groups. Successfully induced osteoblast-like cells were identified by an obvious erythrine area and high expression of collagen I and osteocalcin in ADMSCs transfected with hBMP-7. Thus, ADMSCs can be successfully differentiated into osteoblast-like cells by hBMP-7 induction in vitro.

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“…Washing procedure, immunomagnetic separation or flow cytometric sorting may be used for purification of ADSCs from SVF. However, specific cell surface markers can be modified by the cell culturing procedure and by the number of passages [2]. ADSCs may differentiate not only into adipocytes, osteoblasts, and chondrocytes but also into myocytes, hepatocytes, endothelial cells and neuronal cells [13].…”

Section: Actual State Of Knowledgementioning

confidence: 99%

“…Bone tissue engineering has been considered as the alternative solution to bone rebuilding. One of the crucial purposes of this approach is to seed patient osteoprogenitor cells onto biomaterial and to enhance osteogenic differentiation of stem cells within the scaffold in in vitro conditions in order to obtain a clinically applicable bone construct [1][2].…”

Section: Introduction and Objectivementioning

confidence: 99%

Comparison of osteogenic differentiation ability between bone marrow-derived mesenchymal stem cells and adipose tissue-derived mesenchymal stem cells

Kazimierczak¹,

Syta²,

Przekora³

et al. 2018

Med Og Nauk Zdr.

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Adipose mesenchymal stem cells in the field of bone tissue engineering

Abstract: Bone tissue engineering represents one of the most challenging emergent fields for scientists and clinicians.

Cited by 79 publications

References 104 publications

Three dimensionalde novomicro bone marrow and its versatile application in drug screening and regenerative medicine

Three dimensionalde novomicro bone marrow and its versatile application in drug screening and regenerative medicine

hBMP-7 induces the differentiation of adipose-derived mesenchymal stem cells into osteoblast-like cells

Comparison of osteogenic differentiation ability between bone marrow-derived mesenchymal stem cells and adipose tissue-derived mesenchymal stem cells

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