Adipose Stem Cells as Alternatives for Bone Marrow Mesenchymal Stem Cells in Oral Ulcer Healing

Aly, Lobna Abdel Aziz; Menoufy, Hala El-; Ragae, Alyaa; Rashed, Laila Ahmed; Sabry, Dina

doi:10.15283/ijsc.2012.5.2.104

Cited by 11 publications

(6 citation statements)

References 29 publications

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“…The results of our research were identical to the previous reports [46][47][48], which proved that ADSCs enhance corneal epithelial and stromal wound healing after alkali burns injuries through ameliorating inflammation by their anti-inflammatory effects and modulating immunity. Other studies stated that ADSCs had the ability to exert paracrine effects by secretion of antioxidant enzymes and many different cytokines besides their anti-apoptotic and anti-scarring properties.…”

Section: The Light and Electron Microscopic Examination Of Corneal Sesupporting

confidence: 91%

Comparative Light and Electron Microscopic Study on the Therapeutic Efficacy of Adipose Derived Stem Cells Versus Exosomes for Experimentally Induced Acute Corneal Injuries in Rats

El-Mahalaway¹,

El-Azab²,

Abdrabbo³

et al. 2018

J Stem Cell Res Ther

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Background: Corneal alkali injuries are considered a common ophthalmologic emergency with many serious complications. Adipose Tissue-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells derived Exosomes (MSCs-EX) are promising approaches in regenerative therapies. Objective: The aim of this study is to evaluate the efficacy of ADSCs versus MSCs-EX on experimentally alkaliinduced corneal injuries in rats. Materials and Methods: 40 adult male albino rats were divided into four groups, that is, Group I: control rats, Group II: alkali burn rats, Group III: ADSCs treated rats after corneal alkali burn, Group IV: MSCs-EX treated rats after corneal alkali burn. Cornea specimens were taken and processed for histological examination. Results: Group II showed obvious changes such as erosion with focal loss of some superficial epithelial cells, while the basal and intermediate epithelial cell layers had vacuolated cytoplasm with pyknotic nuclei. The substantia propria contained irregularly arranged collagen fibers with wide spacing, keratocytes, and congested blood vessels. Descemet's membrane appeared thin and degenerated with focal loss of some endothelial cells. Ultrastructural examination showed degenerated apical squamous cells with loss of microvilli on their free surface. The basal cells had irregular contour with widening of intracellular space between cells with multiple cytoplasmic vacuoles. The substantia propria showed disorganized collagen fibers with degenerated keratocytes and mononuclear cellular infiltration. Groups III and IV showed improvement of the histological and electron microscopic changes described in group II. Conclusion: ADSCs and MSCs-EX can improve corneal alkali burn injuries and prevent their complications. Exosomes are a more promising therapeutic approach.

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Section: The Light and Electron Microscopic Examination Of Corneal Sesupporting

confidence: 91%

Comparative Light and Electron Microscopic Study on the Therapeutic Efficacy of Adipose Derived Stem Cells Versus Exosomes for Experimentally Induced Acute Corneal Injuries in Rats

El-Mahalaway¹,

El-Azab²,

Abdrabbo³

et al. 2018

J Stem Cell Res Ther

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“…The use of stem cells is becoming increasingly important in veterinary medicine. Mesenchymal stem cells (MSCs) have been shown to improve tissue repair in oral ulcers [ 1 , 2 ] and bone defects [ 3 – 6 ], as well as in dogs with osteoarthritis of the coxofemoral and elbow joint [ 7 – 10 ]. MSCs have also been used in canine central nervous system to treat spinal cord injury [ 11 – 14 ] and ischemic brain infarction [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Behaviour of adipose-derived canine mesenchymal stem cells after superparamagnetic iron oxide nanoparticles labelling for magnetic resonance imaging

et al. 2016

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BackgroundTherapy with mesenchymal stem cells (MSCs) has been reported to provide beneficial effects in the treatment of neurological and orthopaedic disorders in dogs. The exact mechanism of action is poorly understood. Magnetic resonance imaging (MRI) gives the opportunity to observe MSCs after clinical administration. To visualise MSCs with the help of MRI, labelling with an MRI contrast agent is necessary. However, it must be clarified whether there is any negative influence on cell function and viability after labelling prior to clinical administration.ResultsFor the purpose of the study, seven samples with canine adipose-derived stem cells were incubated with superparamagnetic iron oxide nanoparticles (SPIO: 319.2 μg/mL Fe) for 24 h. The internalisation of the iron particles occurred via endocytosis. SPIO particles were localized as free clusters in the cytoplasm or within lysosomes depending on the time of investigation. The efficiency of the labelling was investigated using Prussian blue staining and MACS assay. After 3 weeks the percentage of SPIO labelled canine stem cells decreased. Phalloidin staining showed no negative effect on the cytoskeleton. Labelled cells underwent osteogenic and adipogenic differentiation. Chondrogenic differentiation occurred to a lesser extent compared with a control sample. MTT-Test and wound healing assay showed no influence of labelling on the proliferation. The duration of SPIO labelling was assessed using a 1 Tesla clinical MRI scanner and T2 weighted turbo spin echo and T2 weighted gradient echo MRI sequences 1, 2 and 3 weeks after labelling. The hypointensity caused by SPIO lasted for 3 weeks in both sequences.ConclusionsAn Endorem labelling concentration of 319.2 μg/mL Fe (448 μg/mL SPIO) had no adverse effects on the viability of canine ASCs. Therefore, this contrast agent could be used as a model for iron oxide labelling agents. However, the tracking ability in vivo has to be evaluated in further studies.

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“…ADSCs were first isolated in dogs from SCAT [ 99 ]. Since then, several studies have isolated and characterized ADSCs from various anatomical locations in the visceral region (VAT), namely falciform ligament, omentum, perirenal, periovarian, and mesenteric regions [ 5 , 41 , 42 , 44 , 48 , 55 , 56 , 57 , 61 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. It is important to note that the morphological and functional characteristics of adipose tissue differ based on whether it originates from the SCAT or VAT regions [ 36 , 37 ].…”

Section: Harvesting Location Influence On Adscs’ Biological Profilementioning

confidence: 99%

Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells’ Biological Profile and Osteogenic Potential in Companion Animals

Ferreira-Baptista,

Ferreira,

Fernandes

et al. 2023

Veterinary Sciences

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Adipose tissue-derived stromal cells (ADSCs) have generated considerable interest in the field of veterinary medicine, particularly for their potential in therapeutic strategies focused on bone regeneration. These cells possess unique biological characteristics, including their regenerative capacity and their ability to produce bioactive molecules. However, it is crucial to recognize that the characteristics of ADSCs can vary depending on the animal species and the site from which they are derived, such as the subcutaneous and visceral regions (SCAT and VAT, respectively). Thus, the present work aimed to comprehensively review the different traits of ADSCs isolated from diverse anatomical sites in companion animals, i.e., dogs, cats, and horses, in terms of immunophenotype, morphology, proliferation, and osteogenic differentiation potential. The findings indicate that the immunophenotype, proliferation, and osteogenic potential of ADSCs differ according to tissue origin and species. Generally, the proliferation rate is higher in VAT-derived ADSCs in dogs and horses, whereas in cats, the proliferation rate appears to be similar in both cells isolated from SCAT and VAT regions. In terms of osteogenic differentiation potential, VAT-derived ADSCs demonstrate the highest capability in cats, whereas SCAT-derived ADSCs exhibit superior potential in horses. Interestingly, in dogs, VAT-derived cells appear to have greater potential than those isolated from SCAT. Within the VAT, ADSCs derived from the falciform ligament and omentum show increased osteogenic potential, compared to cells isolated from other anatomical locations. Consequently, considering these disparities, optimizing isolation protocols becomes pivotal, tailoring them to the specific target species and therapeutic aims, and judiciously selecting the anatomical site for ADSC isolation. This approach holds promise to enhance the efficacy of ADSCs-based bone regenerative therapies.

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Adipose Stem Cells as Alternatives for Bone Marrow Mesenchymal Stem Cells in Oral Ulcer Healing

Cited by 11 publications

References 29 publications

Comparative Light and Electron Microscopic Study on the Therapeutic Efficacy of Adipose Derived Stem Cells Versus Exosomes for Experimentally Induced Acute Corneal Injuries in Rats

Comparative Light and Electron Microscopic Study on the Therapeutic Efficacy of Adipose Derived Stem Cells Versus Exosomes for Experimentally Induced Acute Corneal Injuries in Rats

Behaviour of adipose-derived canine mesenchymal stem cells after superparamagnetic iron oxide nanoparticles labelling for magnetic resonance imaging

Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells’ Biological Profile and Osteogenic Potential in Companion Animals

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