Adipose stem cells in tissue regeneration and repair: From bench to bedside

Dong, Lei; Li, Xiaoyu; Leng, Wenyuan; Guo, Zhenke; Cai, Tianyu; Ji, Xing; Xu, Chunru; Zhu, Zhenpeng; Lin, Jian

doi:10.1016/j.reth.2023.09.014

Cited by 19 publications

(4 citation statements)

References 159 publications

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“…Combined with the scaffold, we used adult stem cells, which are mostly used in bone tissue engineering. hASC were chosen because of their promising advantages, such as high potential for proliferation and differentiation, ability to induce the formation of highly mineralized tissue, abundancy, easy accessibility, less immune rejection and high relevance in bone regeneration [54]. Predifferentiated hASC have demonstrated their ability to form bone when coupled with different scaffolds in vitro and in vivo [55].…”

Section: Discussionmentioning

confidence: 99%

Bioactive gelatin-sheets as novel biopapers to support prevascularization organized by laser-assisted bioprinting for bone tissue engineering

Kérourédan,

Washio,

Handschin

et al. 2024

Biomed. Mater.

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Despite significant advances in the management of patients with oral cancer, maxillofacial reconstruction after ablative surgery remains a clinical challenge. In bone tissue engineering, biofabrication strategies have been proposed as promising alternatives to solve issues associated with current therapies and to produce bone substitutes that mimic both the structure and function of native bone. Among them, Laser-Assisted Bioprinting (LAB) has emerged as a relevant biofabrication method to print living cells and biomaterials with micrometric resolution onto a receiving substrate, also called “biopaper”. Recent studies have demonstrated the benefits of prevascularization using LAB to promote vascularization and bone regeneration, but mechanical and biological optimization of the biopaper are needed. The aim of this study was to apply gelatin-sheet fabrication process to the development of a novel biopaper able to support prevascularization organized by LAB for bone tissue engineering applications. Gelatin-based sheets incorporating bioactive glasses (BG) were produced using various freezing methods and crosslinking (CL) parameters. The different formulations were characterized in terms of microstructural, physical, mechanical, and biological properties in monoculture and coculture. Based on multi-criteria analysis, a rank scoring method was used to identify the most relevant formulations. The selected biopaper underwent additional characterization regarding its ability to support mineralization and vasculogenesis, its bioactivity potential and in vivo degradability. The biopaper “Gel5wt% BG1wt% - slow freezing – CL160°C 24h” was selected as the best candidate, due to its suitable properties including high porosity (91,69+/-1,55%), swelling ratio (91,61+/-0,60%), Young modulus (3,97x104 +/- 0,97x104 Pa) but also great cytocompatibility, osteogenesis and bioactivity properties. The preorganization of HUVEC using LAB onto this new biopaper led to the formation of microvascular networks. This biopaper was also shown to be compatible with 3D-moulding and 3D-stacking strategies. This work allowed the development of a novel biopaper adapted to LAB with great potential for vascularized bone biofabrication.

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Section: Discussionmentioning

confidence: 99%

Bioactive gelatin-sheets as novel biopapers to support prevascularization organized by laser-assisted bioprinting for bone tissue engineering

Kérourédan,

Washio,

Handschin

et al. 2024

Biomed. Mater.

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“…This discrepancy may suggest that neovascularization and/or ECM compensation are still insufficient; otherwise, crucial factors, rather than neovascularization and ECM compensation, may exist in a rat model. Hence, the combination of GHNF with adipose-derived stem cells (ADSCs), which secrete various growth factors and effectively induce neovascularization [ 37 , 38 , 39 , 40 ], would be one option.…”

Section: Discussionmentioning

confidence: 99%

A Gelatin Hydrogel Nonwoven Fabric Enhances Subcutaneous Islet Engraftment in Rats

Saito,

Inagaki,

Nakamura

et al. 2023

Cells

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Although subcutaneous islet transplantation has many advantages, the subcutaneous space is poor in vessels and transplant efficiency is still low in animal models, except in mice. Subcutaneous islet transplantation using a two-step approach has been proposed, in which a favorable cavity is first prepared using various materials, followed by islet transplantation into the preformed cavity. We previously reported the efficacy of pretreatment using gelatin hydrogel nonwoven fabric (GHNF), and the length of the pretreatment period influenced the results in a mouse model. We investigated whether the preimplantation of GHNF could improve the subcutaneous islet transplantation outcomes in a rat model. GHNF sheets sandwiching a silicone spacer (GHNF group) and silicone spacers without GHNF sheets (control group) were implanted into the subcutaneous space of recipients three weeks before islet transplantation, and diabetes was induced seven days before islet transplantation. Syngeneic islets were transplanted into the space where the silicone spacer was removed. Blood glucose levels, glucose tolerance, immunohistochemistry, and neovascularization were evaluated. The GHNF group showed significantly better blood glucose changes than the control group (p < 0.01). The cure rate was significantly higher in the GHNF group (p < 0.05). The number of vWF-positive vessels was significantly higher in the GHNF group (p < 0.01), and lectin angiography showed the same tendency (p < 0.05). The expression of laminin and collagen III around the transplanted islets was also higher in the GHNF group (p < 0.01). GHNF pretreatment was effective in a rat model, and the main mechanisms might be neovascularization and compensation of the extracellular matrices.

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“…Firstly, what works is the multi-directional differentiation ability of MSCs. MSCs may develop into a range of cells, for example, ADSCs can develop into adipocytes, endothelium cells, skeletal muscle cells, and smooth muscle cells to enhance skin wound healing [ 139 , 140 ]. ADSCs are better suited to directed adipocyte growth than stem cells from the rest of the body.…”

Section: The Effect and Possible Mechanism Of Mscs In Improving Wound...mentioning

confidence: 99%

Insights into the role of mesenchymal stem cells in cutaneous medical aesthetics: from basics to clinics

Li,

Liu,

Zhang

et al. 2024

Stem Cell Res Ther

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With the development of the economy and the increasing prevalence of skin problems, cutaneous medical aesthetics are gaining more and more attention. Skin disorders like poor wound healing, aging, and pigmentation have an impact not only on appearance but also on patients with physical and psychological issues, and even impose a significant financial burden on families and society. However, due to the complexities of its occurrence, present treatment options cannot produce optimal outcomes, indicating a dire need for new and effective treatments. Mesenchymal stem cells (MSCs) and their secretomics treatment is a new regenerative medicine therapy that promotes and regulates endogenous stem cell populations and/or replenishes cell pools to achieve tissue homeostasis and regeneration. It has demonstrated remarkable advantages in several skin-related in vivo and in vitro investigations, aiding in the improvement of skin conditions and the promotion of skin aesthetics. As a result, this review gives a complete description of recent scientific breakthroughs in MSCs for skin aesthetics and the limitations of their clinical applications, aiming to provide new ideas for future research and clinical transformation.

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Adipose stem cells in tissue regeneration and repair: From bench to bedside

Cited by 19 publications

References 159 publications

Bioactive gelatin-sheets as novel biopapers to support prevascularization organized by laser-assisted bioprinting for bone tissue engineering

Bioactive gelatin-sheets as novel biopapers to support prevascularization organized by laser-assisted bioprinting for bone tissue engineering

A Gelatin Hydrogel Nonwoven Fabric Enhances Subcutaneous Islet Engraftment in Rats

Insights into the role of mesenchymal stem cells in cutaneous medical aesthetics: from basics to clinics

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