Adipose Tissue - Adequate, Accessible Regenerative Material

Kolaparthy, Lakshmi Kanth; Sanivarapu, Sahitya; Moogla, Srinivas; Kutcham, Rupa Sruthi

doi:10.15283/ijsc.2015.8.2.121

Cited by 64 publications

(47 citation statements)

References 37 publications

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“…AT is a rich source of MSC with ubiquitous availability. Since large numbers of MSC can be obtained from AT by minimal invasive procedures, adMSC have become an important candidates for autologous and allogeneic stem cell based therapies and tissue engineering .Conversely, perinatal tissues such as the amnion, chorion and UC are promising MSC sources due to the young donor age . Although MSCs have been derived from multiple perinatal tissues, UC tissue (Wharton's jelly, WJ) and umbilical cord blood (UCB) are the most frequently used sources, with UC tissue being a richer MSC source compared to UCB .…”

Section: Mesenchymal Stem Cells From Different Human Tissuesmentioning

confidence: 99%

Isolation, cultivation, and characterization of human mesenchymal stem cells

et al. 2017

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Mesenchymal stem cells (MSC) exhibit a high self-renewal capacity, multilineage differentiation potential and immunomodulatory properties. This set of exceptional features makes them an attractive tool for research and clinical application. However, MSC are far from being a uniform cell type, which makes standardization difficult. The exact properties of human MSC (hMSC) can vary greatly depending on multiple parameters including tissue source, isolation method and medium composition. In this review we address the most important influence factors. We highlight variations in the differentiation potential of MSC from different tissue sources. Furthermore, we compare enzymatic isolation strategies with explants cultures focusing on adipose tissue and umbilical cords as two relevant examples. Additionally, we address effects of medium composition and serum supplementation on MSC expansion and differentiation. The lack of standardized methods for hMSC isolation and cultivation mandates careful evaluation of different protocols regarding efficiency and cell quality. MSC characterization based on a set of minimal criteria defined by the International Society for Cellular Therapy is a widely accepted practice, and additional testing for MSC functionality can provide valuable supplementary information. The MSC secretome has been identified as an important signaling mechanism to affect other cells. In this context, extracellular vesicles (EVs) are attracting increasing interest. The thorough characterization of MSC-derived EVs and their interaction with target cells is a crucial step toward a more complete understanding of MSC-derived EV functionality. Here, we focus on flow cytometric approaches to characterize free as well as cell bound EVs and address potential differences in the bioactivity of EVs derived from stem cells from different sources. © 2017 International Society for Advancement of Cytometry.

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Section: Mesenchymal Stem Cells From Different Human Tissuesmentioning

confidence: 99%

Isolation, cultivation, and characterization of human mesenchymal stem cells

et al. 2017

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“…A population of multipotent progenitors, the adipose-derived stem/stromal cells (ASCs) (long identified as preadipocytes) were identified by various studies including ours to exhibit such abilities (95,204,205,262,275,352). ASCs, as their bone marrow counterpart the mesenchymal stem/stromal cells (MSCs) are endowed with multilineage mesodermal differentiation potentials as well as regenerative abilities, leading to their extensive investigation from a therapeutic and tissue engineering perspective (77, 96,158). Adipose tissue remodeling is frequently reported to be associated with the infiltration of various cell populations (226,329).…”

Section: Circulating Adipose Stem/stromal Cellsmentioning

confidence: 99%

Role of Epicardial Adipose Tissue in Health and Disease: A Matter of Fat?

Gaborit

Sengenès

Ancel

et al. 2017

Comprehensive Physiology

122

109

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Epicardial adipose tissue (EAT) is a small but very biologically active ectopic fat depot that surrounds the heart. Given its rapid metabolism, thermogenic capacity, unique transcriptome, secretory profile, and simply measurability, epicardial fat has drawn increasing attention among researchers attempting to elucidate its putative role in health and cardiovascular diseases. The cellular crosstalk between epicardial adipocytes and cells of the vascular wall or myocytes is high and suggests a local role for this tissue. The balance between protective and proinflammatory/profibrotic cytokines, chemokines, and adipokines released by EAT seem to be a key element in atherogenesis and could represent a future therapeutic target. EAT amount has been found to predict clinical coronary outcomes. EAT can also modulate cardiac structure and function. Its amount has been associated with atrial fibrillation, coronary artery disease, and sleep apnea syndrome. Conversely, a beiging fat profile of EAT has been identified. In this review, we describe the current state of knowledge regarding the anatomy, physiology and pathophysiological role of EAT, and the factors more globally leading to ectopic fat development. We will also highlight the most recent findings on the origin of this ectopic tissue, and its association with cardiac diseases. © 2017 American Physiological Society. Compr Physiol 7:1051-1082, 2017.

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“…The yield of ASC in the stromal vascular fraction of a lipoaspirate can reach 1 to 5%; this percentage differs depending on the harvesting site (Jurgens et al, 2008;Kolaparthy et al, 2015). For comparison, the isolation of bone marrow mesenchymal stem cells requires a painful procedure, and a relatively small percentage of stem cells is present in the bone marrow aspirate (500 times less than ASC; Mizuno, 2009).…”

Section: Introductionmentioning

confidence: 99%

Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells

Douglas

Vandrovcová

Kročilová

et al. 2018

Journal of Dairy Science

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Recently, milk-derived proteins have attracted attention for applications in the biomedical field such as tissue regeneration. Whey protein isolate (WPI), especially its main component β-lactoglobulin, can modulate immunity and acts as an antioxidant, antitumor, antiviral, and antibacterial agent. There are very few reports of the application of WPI in tissue engineering, especially in bone tissue engineering. In this study, we tested the influence of different concentrations of WPI on behavior of human osteoblast-like Saos-2 cells, human adipose tissue-derived stem cells (ASC), and human neonatal dermal fibroblasts (FIB). The positive effect on growth was apparent for Saos-2 cells and FIB but not for ASC. However, the expression of markers characteristic for early osteogenic cell differentiation [type-I collagen (COL1) and alkaline phosphatase (ALP)] as well as ALP activity, increased dose-dependently in ASC. Importantly, Saos-2 cells were able to deposit calcium in the presence of WPI, even in a proliferation medium without other supplements that support osteogenic cell differentiation. The results indicate that, depending on the cell type, WPI can act as an enhancer of cell proliferation and osteogenic differentiation. Therefore, enrichment of biomaterials for bone regeneration with WPI seems a promising approach, especially due to the low cost of WPI.

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Adipose Tissue - Adequate, Accessible Regenerative Material

Cited by 64 publications

References 37 publications

Isolation, cultivation, and characterization of human mesenchymal stem cells

Isolation, cultivation, and characterization of human mesenchymal stem cells

Role of Epicardial Adipose Tissue in Health and Disease: A Matter of Fat?

Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells

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