Adipose tissue can be generated in vitro by using adipocytes from human fat tissue mesenchymal stem cells seeded and cultured on fibrin gel sheet

Tran, Cong Toai; Huynh, Duy Thao; Gargiulo, Ciro; Tran, Le Bao; Huynh, Minh Hang; Nguyen, Khanh; Filgueira, Luis; Strong, D.M.

doi:10.1007/s10561-012-9304-6

Cited by 5 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to have a long-term stable biocompatible interface and keep the manufacturing process straightforward, we chose to modify PLLC surface with fibrin coating because fibrin is an insoluble and fibrous protein formed from blood fibrinogen polymerization and it has been developed in varieties of biomedicine applications. 25,28,29 This well established surface modification achieved scaffold's superior biocompatibility. Yet, it can further enhance tensile strength of the porous materials designed to be readily degradable.…”

Section: Fabrication and Fibrin Coating Of Tubular Scaffoldsmentioning

confidence: 98%

Constitution and in vivo test of micro-porous tubular scaffold for esophageal tissue engineering

Hou

Jin

et al. 2015

J Biomater Appl

View full text Add to dashboard Cite

Current clinical techniques in treating long-gap esophageal defects often lead to complications and high morbidity. Aiming at long-gap synthetic esophageal substitute, we had synthesized a biodegradable copolymer, poly(L-lactide-co-caprolactone) (PLLC), with low glass transition temperature. In this work, we developed a tubular PLLC porous scaffold using a self-designed tubular mold and thermal induced phase separation (TIPS) method. In order to enhance the interaction between tissue and scaffold, fibrin, a natural fibrous protein derived from blood fibrinogen, was coated on the scaffold circumferential surface. The fibrin density was measured to be 1.23 ± 0.04 mg/cm(2). Primary epithelial cell culture demonstrated the improved in vitro biocompatibility. In animal study with partial scaffold implantation, in situ mucosa regeneration was observed along the degradation of the scaffold. These indicate that fibrin incorporated PLLC scaffold can greatly improve epithelial regeneration in esophagus repair, therefore serve as a good candidate for long-term evaluation of post-implantation at excision site.

show abstract

Section: Fabrication and Fibrin Coating Of Tubular Scaffoldsmentioning

confidence: 98%

Constitution and in vivo test of micro-porous tubular scaffold for esophageal tissue engineering

Hou

Jin

et al. 2015

J Biomater Appl

View full text Add to dashboard Cite

show abstract

“…The hADSCs were from an available cell line and were isolated, cultured, and expanded according to the protocol described previously (Tran et al, 2013). hADSCs were determined to positively express CD13, CD44, CD73, CD90, CD105, and CD166, and they differentiated into adipocytes after culturing for 21 days in adipogenic medium (Tran et al, 2013). In this study, hADSCs at the 4th passage were used.…”

Section: Chondrogenic Differentiationmentioning

confidence: 99%

In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

Nguyen¹,

Doan²,

Tran³

2019

Turk J Biol

View full text Add to dashboard Cite

Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation of hADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14 days of chondrogenic induction. COMP as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen (Col10a1) expression was stable up to day 14. However, minor expression of aggrecan was found. Taken together, these results indicate that tBP is a potential scaffold for hADSCs for cartilage tissue engineering.Key words: Bovine pericardium, scaffold, adipose-derived stem cells, chondrogenic differentiation, cartilage regeneration, augmentation rhinoplasty

show abstract

“…Besides this group, some other groups in Vietnam have also been involved in stem cell research. One stem cell group, headed by Dr. Tran Cong Toai, at Pham Ngoc Thach Medicine University (HCMC), has investigated procedures to collect and expand stem cells in vitro from bone marrow and adipose tissues [50][51][52]. This group has also conducted studies on culturing and differentiating MSCs on scaffold in order to produce bone [53].…”

Section: Stem Cell Milestone In Viet Nammentioning

confidence: 99%

An evolution of stem cell research and therapy in Viet Nam

Pham

Huynh

et al. 2018

Prog. Stem Cell

View full text Add to dashboard Cite

Stem cell research and therapy are one of the most attractive studies in the biomedicine. Not only in the bench, nowadays stem cells but also become the bustling industry. In Vietnam, biomedical scientists started to study and apply stem cells since 1995. From that, Vietnamese scientists got some significant achievements in stem cell research and therapy, especially in stem cell therapy for disease treatment. This report aimed to provide an overview of stem cell research and therapy from 1995 to date. Stem cell research activities were collected and analyzed based on the publications, projects about stem cells in some databases including Web of Science, Google Scholar, Embase, and national scientific information. The results showed that stem cell research and therapy significantly increased from 2009 to date with more publications about stem cells and more clinical applications. With this growth rate, we hope that Vietnam can develop the stem cell industry and become one the stem cell center in the Asian and the world.

show abstract

Adipose tissue can be generated in vitro by using adipocytes from human fat tissue mesenchymal stem cells seeded and cultured on fibrin gel sheet

Cited by 5 publications

References 31 publications

Constitution and in vivo test of micro-porous tubular scaffold for esophageal tissue engineering

Constitution and in vivo test of micro-porous tubular scaffold for esophageal tissue engineering

In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

An evolution of stem cell research and therapy in Viet Nam

Contact Info

Product

Resources

About