Adipose Tissue-Derived Multi-lineage Progenitor Cells as a Promising Tool for In Situ Stem Cell Therapy

Okura, Hanayuki; Saga, Ayami; Soeda, Mayumi; Ichinose, Akihiro; Matsuyama, Akifumi

doi:10.2174/2211542011201010054

Cited by 2 publications

(2 citation statements)

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“…Fifty‐nine full text studies were assessed on eligibility criteria. Fourteen studies were excluded based on the use of a nonintraoperative protocol for isolation as experimental method (Al Battah et al ., ; Carvalho et al ., ; Chen et al ., ; Condé‐Green and Lamblet ; Doi et al ., ; Escobedo‐Lucea et al ., ; Fink et al ., ; Jiang et al ., ; Okura et al ., ; Pilgaard et al ., ; Seaman et al ., ; Siciliano et al ., ; Vykoukal et al ., ; Yoshimura et al ., ). Seven studies described isolation protocols in general but gave no results (Bernacki et al ., ; Buehrer and Cheatham ; Dubois et al ., ; Hicok and Hedrick, ; Yu et al ., ; Zachar et al ., ; Zhu et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Dongen

Tuin

Spiekman

et al. 2017

J Tissue Eng Regen Med

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Intraoperative application of the stromal vascular fraction (SVF) of adipose tissue requires a fast and efficient isolation procedure of adipose tissue. This review was performed to systematically assess and compare procedures currently used for the intraoperative isolation of cellular SVF (cSVF) and tissue SVF (tSVF) that still contain the extracellular matrix. Pubmed, EMBASE and the Cochrane central register of controlled trials databases were searched for studies that compare procedures for intraoperative isolation of SVF (searched 28 September 2016). Outcomes of interest were cell yield, viability of cells, composition of SVF, duration, cost and procedure characteristics. Procedures were subdivided into procedures resulting in a cSVF or tSVF. Thirteen out of 3038 studies, evaluating 18 intraoperative isolation procedures, were considered eligible. In general, cSVF and tSVF intraoperative isolation procedures had similar cell yield, cell viability and SVF composition compared to a nonintraoperative (i.e. culture laboratory-based collagenase protocol) control group within the same studies. The majority of intraoperative isolation procedures are less time consuming than nonintraoperative control groups, however. Intraoperative isolation procedures are less time-consuming than nonintraoperative control groups with similar cell yield, viability of cells and composition of SVF, and therefore more suitable for use in the clinic. Nevertheless, none of the intraoperative isolation procedures could be designated as the preferred procedure to isolate SVF.

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Section: Resultsmentioning

confidence: 99%

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Dongen

Tuin

Spiekman

et al. 2017

J Tissue Eng Regen Med

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“…Thus, the use of adult stem cells for cell therapy has been widely investigated. Adipose-derived stem cells (ADSCs) are a type of adult mesenchymal stem cell with the potential for multi-lineage differentiation to chondrocytes, myocytes, adipocytes, or osteoblasts [ 23 – 25 ]. ADSCs are abundant and easy to obtain and can be cultured without supplementary cytokines.…”

Section: Introductionmentioning

confidence: 99%

Cellular Behavior of Human Adipose-Derived Stem Cells on Wettable Gradient Polyethylene Surfaces

Ahn

Lee

et al. 2014

IJMS

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Appropriate surface wettability and roughness of biomaterials is an important factor in cell attachment and proliferation. In this study, we investigated the correlation between surface wettability and roughness, and biological response in human adipose-derived stem cells (hADSCs). We prepared wettable and rough gradient polyethylene (PE) surfaces by increasing the power of a radio frequency corona discharge apparatus with knife-type electrodes over a moving sample bed. The PE changed gradually from hydrophobic and smooth surfaces to hydrophilic (water contact angle, 90º to ~50º) and rough (80 to ~120 nm) surfaces as the power increased. We found that hADSCs adhered better to highly hydrophilic and rough surfaces and showed broadly stretched morphology compared with that on hydrophobic and smooth surfaces. The proliferation of hADSCs on hydrophilic and rough surfaces was also higher than that on hydrophobic and smooth surfaces. Furthermore, integrin beta 1 gene expression, an indicator of attachment, and heat shock protein 70 gene expression were high on hydrophobic and smooth surfaces. These results indicate that the cellular behavior of hADSCs on gradient surface depends on surface properties, wettability and roughness.

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Adipose Tissue-Derived Multi-lineage Progenitor Cells as a Promising Tool for In Situ Stem Cell Therapy

Cited by 2 publications

References 0 publications

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Cellular Behavior of Human Adipose-Derived Stem Cells on Wettable Gradient Polyethylene Surfaces

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