Adipose tissue-derived stem cells in a fibrin implant enhance neovascularization in a peritoneal grafting site: a potential way to improve ovarian tissue transplantation

Manavella, Diego; Cacciottola, Luciana; Desmet, Céline; Jordan, Bénédicte F.; Donnez, Jacques; Amorim, Christiani Andrade; Dolmans, Marie-Madeleine

doi:10.1093/humrep/dex374

Cited by 64 publications

(57 citation statements)

References 44 publications

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“…The inner peritoneal surface was first scratched and the scaffold gently sutured with two to three cardinal stitches using 7/0 Prolene. Incorporation of cells into the Fi implants and LiPc crystal insertion are described in detail by Manavella et al . The remaining control mice (n = 6) were grafted with empty Fi implants containing only LiPc crystals.…”

Section: Grafting Site Preparation With Ascsmentioning

confidence: 99%

“…ASCs were identified and labeled in the xenotransplantation site using anti‐human vimentin, a cytoskeletal protein and marker of cells of mesenchymal origin. Furthermore, anti‐human Ki67 immunostaining was performed to quantify the number of proliferating ASCs …”

Section: Grafting Site Preparation With Ascsmentioning

confidence: 99%

“…Manavella et al previously demonstrated that high concentrations of ASCs delivered inside a fibrin (Fi) implant designed to prepare the OT transplantation site 14 days prior to grafting resulted in better vascularization. In a follow‐up study, the same protocol was applied to test the short‐term effects of a two‐step OT transplantation approach using ASCs, looking to increase follicle survival rates through enhanced graft vascularization …”

Section: Introductionmentioning

confidence: 99%

“…The goal of this review is to describe and discuss our attempts to improve the revascularization process after OT reimplantation, focusing specifically on use of ASCs in this context. To this end, the findings of two studies will be analyzed and discussed separately. The aim of the first study was to develop a peritoneal transplantation site with ideal features for an avascular transplantation procedure in terms of vascularization.…”

Section: Introductionmentioning

confidence: 99%

“…Based on the findings of this first study, the second study was developed, applying this new protocol in a xenografting model using human OT. The goal was to improve graft revascularization in an attempt to increase follicle survival.…”

Section: Introductionmentioning

confidence: 99%

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Translational research aiming to improve survival of ovarian tissue transplants using adipose tissue‐derived stem cells

Dolmans

Cacciottola

Amorim

et al. 2019

Acta Obstet Gynecol Scand

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There is now sufficient evidence to support the feasibility and efficacy of ovarian tissue (OT) cryopreservation and transplantation for both fertility preservation and restoration purposes. However, there are still issues to address regarding the grafting procedure itself, since transplanted tissue suffers massive follicle loss in the early post‐grafting period. To improve follicle survival after transplantation, our group recently developed a two‐step transplantation technique for OT transplantation in a xenografting model using adipose tissue‐derived stem cells (ASCs). The aim of this narrative review is to describe and discuss the previously reported findings. ASCs were initially characterized by flow cytometry as positive for CD29, CD44, CD73, CD90, CD105 and CD166 (>95%) and negative for CD34, CD14, CD31, CD45 and Lin1. ASCs were used in a model of xenotransplantation, were they were embedded in a fibrin scaffold and transplanted to the peritoneum of immunodeficient mice. The goal of the first step was to increase levels of partial pressure of oxygen (pO2) and revascularization in the peritoneal transplantation site for further OT transplantation. As ASCs showed the ability to differentiate into endothelial‐like cells and vessels in our model, OT transplantation was then performed with ASC grafts in a controlled experiment. At 7 days post‐transplantation, the ASC group showed: (1) significantly higher pO2 levels; (2) significantly greater human and murine CD34‐positive endothelial areas; (3) significantly higher primordial follicle survival rates; (4) and significantly lower numbers of apoptotic follicles compared with the control group. Our research model demonstrates that by adding ASCs to a fibrin scaffold before OT transplantation, faster and better graft reoxygenation and revascularization may be obtained, resulting in increased follicle survival and reduced follicle apoptosis.

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Section: Grafting Site Preparation With Ascsmentioning

confidence: 99%

Section: Grafting Site Preparation With Ascsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Translational research aiming to improve survival of ovarian tissue transplants using adipose tissue‐derived stem cells

Dolmans

Cacciottola

Amorim

et al. 2019

Acta Obstet Gynecol Scand

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show abstract

Current state and future possibilities of ovarian tissue transplantation

Takae

Suzuki

2019

Reprod Medicine & Biology

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Background As a result of recent developments in cancer treatment, cancer survivorship and survivors' quality of life have been emphasized. Although ovarian tissue cryopreservation (OTC) is an experimental technique, it would be the sole technique for fertility preservation treatment for girls with malignant disease. Indeed, OTC requires ovarian tissue transplantation (OTT) for conception. As for OTC, there is room to investigate OTT. The present review focused on the current state and progress of OTT. Method The literature regarding OTT, which is currently under development, was reviewed. Main findings To improve the outcome of OTT, both efficacy and safety are important. Good surgical technique and the optimal site are important surgical factors, with orthotopic transplantation increasing. Treatment of growth factors, gonadotropins, antioxidants, apoptosis suppression factors, and cell therapy may improve the efficacy of OTT by inducing neo‐angiogenesis and preventing damage. Artificial ovaries, complete in vitro primordial follicle culture technique, and non‐invasive ovarian imaging techniques, such as optical coherence tomography, to select the best ovarian tissue are future possibilities. Conclusion Improving neo‐angiogenesis and preventing damage with optimization, as well as investigation of future techniques, may bring us to the next stage of a fertility preservation strategy.

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Overview of Fertility Preservation Approaches in Cancer Patients

Demeestere

2019

Fertility Challenges and Solutions in Women With Cancer

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Adipose tissue-derived stem cells in a fibrin implant enhance neovascularization in a peritoneal grafting site: a potential way to improve ovarian tissue transplantation

Cited by 64 publications

References 44 publications

Translational research aiming to improve survival of ovarian tissue transplants using adipose tissue‐derived stem cells

Translational research aiming to improve survival of ovarian tissue transplants using adipose tissue‐derived stem cells

Current state and future possibilities of ovarian tissue transplantation

Overview of Fertility Preservation Approaches in Cancer Patients

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