Adipose Tissue Uses in Peripheral Nerve Surgery

Podsednik, Allison; Cabrejo, Raysa; Rosen, Joseph M.

doi:10.3390/ijms23020644

Cited by 12 publications

(8 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrogels may play a greater role in peripheral nerve crush injuries [ 83 ]. In addition, it should be pointed out that fat grafting has also become increasingly popular in peripheral nerve surgery in recent years [ 84 ], which is not contradictory to the operation in this study to reduce the fat content of ANXs by means of scCO2 extraction. On the one hand, the function of adipose tissue in fat grafting is mainly achieved by the adipose-derived stem cells (ADSCs) it contains, which can differentiate into many lineages and secrete neurotrophic and angiogenic factors [ 85 , 86 ].…”

Section: Discussionmentioning

confidence: 92%

Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Wei

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 92%

Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Wei

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

“…In this study, we also attempted to restore systemic lipid metabolic homeostasis and investigated its rescue effect on neurogenesis [ 18 , 35 ]. As well known, AT not only served as an organ for energy storage but also as the endocrine organ to regulate metabolic homeostasis [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Adipose transplantation improves olfactory function and neurogenesis via PKCα-involved lipid metabolism in Seipin Knockout mice

Yang,

Zhao

et al. 2023

Stem Cell Res Ther

View full text Add to dashboard Cite

Background Lipodystrophy-associated metabolic disorders caused by Seipin deficiency lead to not only severe lipodystrophy but also neurological disorders. However, the underlying mechanism of Seipin deficiency-induced neuropathy is not well elucidated, and the possible restorative strategy needs to be explored. Methods In the present study, we used Seipin knockout (KO) mice, combined with transcriptome analysis, mass spectrometry imaging, neurobehavior test, and cellular and molecular assay to investigate the systemic lipid metabolic abnormalities in lipodystrophic mice model and their effects on adult neurogenesis in the subventricular zone (SVZ) and olfactory function. After subcutaneous adipose tissue (AT) transplantation, metabolic and neurological function was measured in Seipin KO mice to clarify whether restoring lipid metabolic homeostasis would improve neurobehavior. Results It was found that Seipin KO mice presented the ectopic accumulation of lipids in the lateral ventricle, accompanied by decreased neurogenesis in adult SVZ, diminished new neuron formation in the olfactory bulb, and impaired olfactory-related memory. Transcriptome analysis showed that the differentially expressed genes (DEGs) in SVZ of adult Seipin KO mice were significantly enriched in lipid metabolism. Mass spectrometry imaging showed that the levels of glycerophospholipid and diglyceride (DG) were significantly increased. Furthermore, we found that AT transplantation rescued the abnormality of peripheral metabolism in Seipin KO mice and ameliorated the ectopic lipid accumulation, concomitant with restoration of the SVZ neurogenesis and olfactory function. Mechanistically, PKCα expression was up-regulated in SVZ tissues of Seipin KO mice, which may be a potential mediator between lipid dysregulation and neurological disorder. DG analogue (Dic8) can up-regulate PKCα and inhibit the proliferation and differentiation of neural stem cells (NSCs) in vitro, while PKCα inhibitor can block this effect. Conclusion This study demonstrates that Seipin deficiency can lead to systemic lipid disorder with concomitant SVZ neurogenesis and impaired olfactory memory. However, AT restores lipid homeostasis and neurogenesis. PKCα is a key mediator mediating Seipin KO-induced abnormal lipid metabolism and impaired neurogenesis in the SVZ, and inhibition of PKCα can restore the impaired neurogenesis. This work reveals the underlying mechanism of Seipin deficiency-induced neurological dysfunction and provides new ideas for the treatment of neurological dysfunction caused by metabolic disorders.

show abstract

“…Standard reconstructive techniques use autograft for filling the gap between the proximal stump and the distal nerve in facial nerve reanimation[ 4 ]. Studies have shown that autograft use tend to be superior to acellular grafts in these cases, in which sensory nerves are considered as the gold standard graft for motor nerves reconstruction[ 8 ]. In designing our meta-analysis, we thus included only studies comparing autografts and stem cells for facial nerve reanimation in rats.…”

Section: Discussionmentioning

confidence: 99%

Role of stem cells-based in facial nerve reanimation: A meta-analysis of histological and neurophysiological outcomes

Ricciardi¹,

Pucci²,

Piazza³

et al. 2022

WJSC

View full text Add to dashboard Cite

BACKGROUND Treatments involving stem cell (SC) usage represent novel and potentially interesting alternatives in facial nerve reanimation. Current literature includes the use of SC in animal model studies to promote graft survival by enhancing nerve fiber growth, spreading, myelinization, in addition to limiting fibrotic dege neration after surgery. However, the effectiveness of the clinical use of SC in facial nerve reanimation has not been clarified yet. AIM To investigate the histological, neurophysiological, and functional outcomes in facial reanimation using SC, compared to autograft. METHODS Our study is a systematic review of the literature, consistently conducted according to the preferred reporting items for systematic reviews and meta-analyses statement guidelines. The review question was: In facial nerve reanimation on rats, has the use of stem cells revealed as effective when compared to autograft, in terms of histological, neurophysiological, and functional outcomes? Random-effect meta-analysis was conducted on histological and neurophysiological data from the included comparative studies. RESULTS After screening 148 manuscript, five papers were included in our study. 43 subjects were included in the SC group, while 40 in the autograft group. The meta-analysis showed no significative differences between the two groups in terms of myelin thickness [CI: -0.10 (-0.20, 0.00); I 2 = 29%; P = 0.06], nerve fibers diameter [CI: 0.72 (-0.93, 3.36); I 2 = 72%; P = 0.6], compound muscle action potential amplitude [CI: 1.59 (0.59, 3.77); I 2 = 89%; P = 0.15] and latency [CI: 0.66 (-1.01, 2.32); I 2 = 67%; P = 0.44]. The mean axonal diameter was higher in the autograft group [CI: 0.94 (0.60, 1.27); I 2 = 0%; P ≤ 0.001]. CONCLUSION The role of stem cells in facial reanimation is still relatively poorly studied, in animal models, and available results should not discourage their use in future studies on human subjects.

show abstract

Adipose Tissue Uses in Peripheral Nerve Surgery

Cited by 12 publications

References 63 publications

Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Adipose transplantation improves olfactory function and neurogenesis via PKCα-involved lipid metabolism in Seipin Knockout mice

Role of stem cells-based in facial nerve reanimation: A meta-analysis of histological and neurophysiological outcomes

Contact Info

Product

Resources

About