Adjacent cartilage tissue structure after successful transplantation: a quantitative MRI study using T2 mapping and texture analysis

Abstract

Objectives The aim of this study was to assess the texture of repair tissue and tissue adjacent to the repair site after matrix-associated chondrocyte transplantation (MACT) of the knee using gray-level co-occurrence matrix (GLCM) texture analysis of T2 quantitative maps. Methods Twenty patients derived from the MRI sub-study of multicenter, single-arm phase III study underwent examination on a 3 T MR scanner, including a T2 mapping sequence 12 and 24 months after MACT. Changes between the time points in mean T2 values and 20 GLCM features were assessed for repair tissue, adjacent tissue, and reference cartilage. Differences in T2 values and selected GLCM features between the three cartilage sites at two time points were analyzed using linear mixed-effect models. Results A significant decrease in T2 values after MACT, between time points, was observed only in repair cartilage (p < 0.001). Models showed significant differences in GLCM features between repair tissue and reference cartilage, namely, autocorrelation (p < 0.001), correlation (p = 0.015), homogeneity (p = 0.002), contrast (p < 0.001), and difference entropy (p = 0.047). The effect of time was significant in a majority of models with regard to GLCM features (except autocorrelation) (p ≤ 0.001). Values in repair and adjacent tissue became similar to reference tissue over time. Conclusions GLCM is a useful add-on to T2 mapping in the evaluation of knee cartilage after MACT by increasing the sensitivity to changes in cartilage structure. The results suggest that cartilage tissue adjacent to the repair site heals along with the cartilage implant. Key Points • GLCM is a useful add-on to T2mapping in the evaluation of knee cartilage after MACT by increasing the sensitivity to changes in cartilage structure. • Repair and adjacent tissue became similar to reference tissue over time. • The results suggest that cartilage tissue adjacent to the repair site heals along with the cartilage implant.

Determining the Relationship between Mechanical Properties and Quantitative Magnetic Resonance Imaging of Joint Soft Tissues Using Patient-Specific Templates

“…When the target tissue is damaged, water may enter the tissue, resulting in a change in the T2 values. Currently, quantitative MRI is used to evaluate damaged articular cartilage in many scenarios, including microfracture [ 25 , 26 ] and cartilage transplantation [ 27 , 28 ]. If strength can be predicted from T2 values, as indicated by the results of this study, determining the timing of load initiation may be possible.…”

Magnetic resonance imaging and ultrasound elastography in the context of preclinical pharmacological research: significance for the 3R principles

“…In a phase I–II trial T 2 reductions upon intra-articular administration of ex vivo expanded autologous mesenchymal stromal cells indicated cartilage regeneration ( Soler et al, 2016 ). Three recent studies demonstrated T 2 reductions after implanting a biomaterial scaffold seeded with chondrocytes ( Li et al, 2021 ; Xu et al, 2021 ; Janacova et al, 2022 ). In two of the studies early efficacy suggesting cartilage repair translated into cartilage T 2 reductions within 3–6 months of implantation, accompanied by improvements in functionality and pain levels ( Li et al, 2021 ; Xu et al, 2021 ).…”

“…In two of the studies early efficacy suggesting cartilage repair translated into cartilage T 2 reductions within 3–6 months of implantation, accompanied by improvements in functionality and pain levels ( Li et al, 2021 ; Xu et al, 2021 ). Texture analysis of T 2 quantitative maps has also been introduced to study cartilage repair ( Janacova et al, 2022 ).…”