Adjustable nanofibers self-assembled from an irregular conformational peptide amphiphile

Qin, Si‐Yong; Jiang, Hua-Fang; Peng, Mengyun; Lei, Qi; Zhuo, Ren‐Xi; Zhang, Xian‐Zheng

doi:10.1039/c4py01237b

Cited by 21 publications

(15 citation statements)

References 44 publications

(41 reference statements)

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“…Two negative peaks at 207 and 220 nm and a positive peak at 191 nm within LELL suggested α-helixes. Conversely, the CD spectra of VEVV showed a positive peak at 195 nm and a negative peak at 215 nm, indicating a β-sheet structure [47,48].…”

Section: Resultsmentioning

confidence: 99%

Hydroxyapatite Formation on Self-Assembling Peptides with Differing Secondary Structures and Their Selective Adsorption for Proteins

Kojima

Nakamura

Lee

et al. 2019

IJMS

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Self-assembling peptides have been employed as biotemplates for biomineralization, as the morphologies and sizes of the inorganic materials can be easily controlled. We synthesized two types of highly ordered self-assembling peptides with different secondary structures and investigated the effects of secondary structures on hydroxyapatite (HAp) biomineralization of peptide templates. All as-synthesized HAp-peptides have a selective protein adsorption capacity for basic protein (e.g., cytochrome c and lysozyme). Moreover, the selectivity was improved as peptide amounts increased. In particular, peptide–HAp templated on β-sheet peptides adsorbed more cytochrome c than peptide–HAp with α-helix structures, due to the greater than 2-times carboxyl group density at their surfaces. It can be expected that self-assembled peptide-templated HAp may be used as carriers for protein immobilization in biosensing and bioseparation applications and as enzyme-stabilizing agents.

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Section: Resultsmentioning

confidence: 99%

Hydroxyapatite Formation on Self-Assembling Peptides with Differing Secondary Structures and Their Selective Adsorption for Proteins

Kojima

Nakamura

Lee

et al. 2019

IJMS

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“…The D-amino acids were taken to construct the targeted peptide and D (KLAKLAK) 2 was synthesized based on SPPS. 26 The free D (KLAKLAK) 2 peptide drug may still suffer from several shortcomings including poor cell penetration, immunogenicity, and even rapid in vivo degradation. 24 Administering the vulnerable agents in a nanoparticle formulation could overcome those drawbacks.…”

Section: Resultsmentioning

confidence: 99%

An innovative pre-targeting strategy for tumor cell specific imaging and therapy

et al. 2015

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A programmed pre-targeting system for tumor cell imaging and targeting therapy was established based on the "biotin-avidin" interaction. In this programmed functional system, transferrin-biotin can be actively captured by tumor cells with the overexpression of transferrin receptors, thus achieving the pre-targeting modality. Depending upon avidin-biotin recognition, the attachment of multivalent FITC-avidin to biotinylated tumor cells not only offered the rapid fluorescence labelling, but also endowed the pre-targeted cells with targeting sites for the specifically designed biotinylated peptide nano-drug. Owing to the successful pre-targeting, tumorous HepG2 and HeLa cells were effectively distinguished from the normal 3T3 cells via fluorescence imaging. In addition, the self-assembled peptide nano-drug resulted in enhanced cell apoptosis in the observed HepG2 cells. The tumor cell specific pre-targeting strategy is applicable for a variety of different imaging and therapeutic agents for tumor treatments.

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“…The self-aggregation of natural CPT drug in solution was firstly judged based on the transformation from transparent solution to sub-transparent solution or hydrogel state, which was generally considered to be a visual evidence for the self-aggregation [ 17–19 ]. After mixing 1 × 10 − 2 M CPT stock DMSO solution with distilled water or PBS and making a concentration of CPT to 2.5 × 10 − 4 M, a sub-transparent solution was immediately formed, which suggested the rapid self-assembly of CPT molecules.…”

Section: Resultsmentioning

confidence: 99%

Self-defensive nano-assemblies from camptothecin-based antitumor drugs

et al. 2015

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Camptothecin (CPT)-based drugs always undergo the reversible, pH-dependent lactone ring-opening reaction, yielding the inactive but toxic carboxylate form. Self-assembly strategy provides an effective route for preserving their bio-stability. In this article, nano-sized self-assemblies from CPT-based antitumor drugs were simply built up by directly diluting the stock dimethylsulfoxide solutions of (S)-(+)-CPT, (S)-10-hydroxyl camptothecin and carboxylic CPT with water/phosphate-buffered saline solution. Because of their different molecular structures in A-ring or modification on the 20-OH group, CPT self-assembled into helical nano-ribbons, whereas 10-hydroxycamptothecin and carboxylic CPT self-aggregated into flat nano-ribbons and cylindric nano-rods, respectively. Attractively, the self-assembly of CPT-based drugs could occur within 1 min at a low concentration of 1 × 10−5 M. Adopting the J-type self-aggregation, self-assemblies were stable in aqueous solution and could effectively protect the CPT-based drugs from hydrolysis, which thereby kept their bioactivity for tumor therapy.

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Adjustable nanofibers self-assembled from an irregular conformational peptide amphiphile

Abstract: A peptide amphiphile adopting an irregular conformation self-assembled into dendritic nanofibers, peacock-feather-like nanofibers, and even parallel nanofibers.

Cited by 21 publications

References 44 publications

Hydroxyapatite Formation on Self-Assembling Peptides with Differing Secondary Structures and Their Selective Adsorption for Proteins

Hydroxyapatite Formation on Self-Assembling Peptides with Differing Secondary Structures and Their Selective Adsorption for Proteins

An innovative pre-targeting strategy for tumor cell specific imaging and therapy

Self-defensive nano-assemblies from camptothecin-based antitumor drugs

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