Adjusting primary screening conditions to obtain reproducible performance of <i>Pichia pastoris</i> clones at different cultivation scales

Wollborn, David; Germer, Andrea; Hußmann, F. K.; Blank, Lars M.; Büchs, Jochen

doi:10.1002/cite.202055107

Cited by 1 publication

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“…After a short lag phase of ~12 h, the OTR increases exponentially reaching a peak at about 7 mmol/L/h. After this peak, the OTR drops sharply, due to glucose depletion (Wollborn et al, 2020), indicating total carbon source consumption, and the end of the cultivation. No further metabolic activity is observed.…”

Section: Resultsmentioning

confidence: 99%

“…After a short lag phase of ~12 h, the OTR increases exponentially reaching a peak at about 7 mmol/L/h. After this peak, the OTR drops sharply, due to glucose depletion (Wollborn et al, 2020), ). The fifth row summarizes the analytical result for the precipitate formed during preparation of the medium, according to the Invitrogen protocol at pH = 6 (2nd row in Figure 1).…”

Section: Nutrient Limitation In Invitrogen Medium Due To Struvite Pre...mentioning

confidence: 99%

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Investigation into struvite precipitation: A commonly encountered problem during fermentations on chemically defined media

Steimann,

Wollborn,

Röck

et al. 2023

Biotech & Bioengineering

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Chemically defined mineral media are widely used in bioprocesses, as these show less batch to batch variation compared with complex media. Nonetheless, the recommended media formulations often lead to the formation of precipitants at elevated pH values. These precipitates are insoluble and reduce the availability of macronutrients to the cells, which can result in limiting growth rates and lower productivity. They can also damage equipment by clogging pipes, hoses, and spargers in stirred tank fermenters. In this study, the observed precipitate was analyzed via X‐ray fluorescence spectroscopy and identified as the magnesium ammonium phosphate salt struvite (MgNH4PO4 × 6H2O). The solubility of struvite crystals is known to be extremely low, causing the macronutrients magnesium, phosphate, and ammonium to be bound in the struvite crystals. Here, it was shown that struvite precipitates can be redissolved under common fermentation conditions. Furthermore, it was found that the struvite particle size distribution has a significant effect on the dissolution kinetics, which directly affects macronutrient availability. At a certain particle size, struvite crystals rapidly dissolved and provided unlimiting growth conditions. Therefore, struvite formation should be considered during media and bioprocess development, to ensure that the dissolution kinetics of struvite are faster than the growth kinetics.

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Section: Resultsmentioning

confidence: 99%

“…After a short lag phase of ~12 h, the OTR increases exponentially reaching a peak at about 7 mmol/L/h. After this peak, the OTR drops sharply, due to glucose depletion (Wollborn et al, 2020), ). The fifth row summarizes the analytical result for the precipitate formed during preparation of the medium, according to the Invitrogen protocol at pH = 6 (2nd row in Figure 1).…”

Section: Nutrient Limitation In Invitrogen Medium Due To Struvite Pre...mentioning

confidence: 99%

Investigation into struvite precipitation: A commonly encountered problem during fermentations on chemically defined media

Steimann,

Wollborn,

Röck

et al. 2023

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

Adjusting primary screening conditions to obtain reproducible performance of Pichia pastoris clones at different cultivation scales

Cited by 1 publication

References 0 publications

Investigation into struvite precipitation: A commonly encountered problem during fermentations on chemically defined media

Investigation into struvite precipitation: A commonly encountered problem during fermentations on chemically defined media

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