Administration of human chorionic gonadotropin at embryo transfer induced ovulation of a first-wave dominant follicle and increased progesterone and transfer pregnancy rates

Wallace, L. D.; Breiner, C.A.; Spell, A.R.; Carter, J.A.; Lamb, G. C.; Stevenson, Jeffrey S.; Breiner, Ryan M.

doi:10.4148/2378-5977.2908

Cited by 3 publications

(5 citation statements)

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“…Beltman et al (2009a) found that supplementing beef heifers with exogenous P4 during metoestrus (P <0.04) or early dioestrus (P< 0.06) had a positive relationship with embryo survival rates. Similar results were described by Wallace et al (2011) who found significantly higher pregnancy rates in P4-supplemented beef heifers treated with human chorionic gonadotropin (hCG 1000 IU) compared to non-treated animals (66.3 vs. 55.5%, respectively, P< 0.001). In the experiment of Beltman et al (2009a) the embryo length assessed at Day 25 was similar in treated and non-treated heifers (29.5 vs. 30.2 cm, respectively).…”

Section: Role Of Progesterone On Embryo Development and Survivalsupporting

confidence: 77%

“…However, when the length was assessed earlier, shortly after MRP (Day 16), the embryos recovered from P4-treated cows were fourfold longer (P<0.01) than embryos from nontreated cows. In addition, the uterine fluid recovered from P4-treated cows, showed a six-fold higher concentration of IFNτ than the uterine fluid from non-treated cows (Wallace et al, 2011). …”

Section: Role Of Progesterone On Embryo Development and Survivalmentioning

confidence: 99%

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63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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Section: Role Of Progesterone On Embryo Development and Survivalsupporting

confidence: 77%

Section: Role Of Progesterone On Embryo Development and Survivalmentioning

confidence: 99%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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“…In animal models, systemic administration of hCG at the time of embryo transfer significantly increases pregnancy rate. 10 Intrauterine hCG injection is associated with significant proimplantation changes of the endometrium during the window of receptivity in normal cycling baboons, and induces a favourable uterine environment for embryo implantation. 11…”

Section: Introductionmentioning

confidence: 99%

“…7,9 In animal models, systemic administration of hCG at the time of embryo transfer significantly increases pregnancy rate. 10 Intrauterine hCG injection is associated with significant proimplantation changes of the endometrium during the window of receptivity in normal cycling baboons, and induces a favourable uterine environment for embryo implantation. 11 Intrauterine injection with hCG (500 IU/ ml) prior to embryo transfer significantly improves rates of implantation and pregnancy in IVF/ICSI (intracytoplasmic sperm injection).…”

Section: Introductionmentioning

confidence: 99%

The efficacy of intrauterine injection of human chorionic gonadotropin before embryo transfer in assisted reproductive cycles: Meta-analysis

Hong

et al. 2015

J Int Med Res

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“…Nevertheless, compared with control, the numerical increase (8%) in pregnancy rate on Day 63 of hCG treatment can be of economic interest. At experiment design, we estimated an increase in pregnancy rate in the range of 5% to 20% based on previous (Chagas e Silva and Lopes-da-Costa, 2005) and published (Nishigai et al, 2002;Wallace et al, 2011) results. Treatment with hCG induced the formation of secondary CL and a significant increase in plasma P 4 concentrations.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of treatments with hCG and carprofen at embryo transfer in a demi-embryo and recipient virgin heifer model

Torres

Silva

Diniz

et al. 2013

Animal

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An in vivo model, combining a low developmental competence embryo (demi-embryo) and a high-fertility recipient (virgin dairy heifer) was used to evaluate the effects of treatment with human chorionic gonadotropin (hCG) and carprofen at embryo transfer (ET) on plasma progesterone (P 4 ) concentrations of recipients and on embryonic growth and survival. Embryos were bisected and each demi-embryo was transferred to a recipient on Day 7 of the estrous cycle. At ET, heifers (n 5 163) were randomly allocated to treatment with hCG (2500 IU im), carprofen (500 mg iv), hCG plus carprofen or to untreated controls. Plasma P 4 concentrations were measured on Days 0, 7, 14 and 21 of all recipients plus on Days 28, 42 and 63 of pregnant recipients. Pregnancy was presumed to be present in recipients with luteal plasma P 4 concentrations until Day 21 and confirmed by using transrectal ultrasonography on Days 28, 42 and 63. Embryonic measurements (crown-rump length and width) were obtained on Day 42. Treatment with hCG induced formation of secondary corpora lutea (CL) in 97% of heifers and increased ( P , 0.01) mean plasma P 4 concentrations of non-pregnant recipients on Day 14 and of pregnant heifers on Days 14 to 63. This was associated to a significant decrease in early embryonic mortality. In contrast, subsequent embryonic losses resulted in a non-significant numerical increase by 8% of pregnancies maintained to Day 63. Therefore, treatment with hCG significantly rescued embryos through the maternal recognition of pregnancy window but was not able to support development thereafter. Treatment with carprofen at ET had no significant effects on plasma P 4 concentrations and rate of embryo mortality. Treatment with hCG plus carprofen at ET induced formation of secondary CL in 90% of heifers but decreased the luteotrophic effect of hCG, resulting in no effect on embryo survival. Low developmental competence embryos showed an intrinsic deficiency in overcoming the maternal recognition of pregnancy challenge and in proceeding to further development until Day 28 of pregnancy, whereas mortality beyond this point was residual. Results on pregnancy rates should be confirmed in further experiments involving a larger sample size. Keywords: demi-embryo, hCG, carprofen, progesterone, bovine Implications Embryonic mortality is a major source of economic loss in the cattle industry, and may occur because of intrinsic embryonic, maternal and management factors. These factors may interact, which makes difficult to study their mechanisms of action and the design of appropriate therapeutic approaches. Herein, we used an in vivo model, considering a compromised embryo and a high-fertility recipient, to evaluate therapeutic strategies designed to enhance embryonic survival. Preliminary results showed that treatment with hCG at embryo transfer may be of interest to decrease embryonic mortality, whereas carprofen had no apparent effect on embryo survival. IntroductionPregnancy loss is a major cause of reproductive failure and of economic waste in...

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Administration of human chorionic gonadotropin at embryo transfer induced ovulation of a first-wave dominant follicle and increased progesterone and transfer pregnancy rates

Cited by 3 publications

References 0 publications

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

The efficacy of intrauterine injection of human chorionic gonadotropin before embryo transfer in assisted reproductive cycles: Meta-analysis

Evaluation of treatments with hCG and carprofen at embryo transfer in a demi-embryo and recipient virgin heifer model

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