Adrenergic, dopaminergic and serotonergic gene expression in low dose, long time insulin and somatotropin treatment to ageing rats: rejuvenation of brain function

Paulose, C S; Sadasivam, Balakrishnan

doi:10.1007/s10522-008-9183-1

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…From our results, the ADRB2 gene expression was inversely correlated with oxidized LDL and triglycerides at 6 h after VOO ingestion, reinforcing the above-mentioned possible protection. Insulin treatment has shown to increase ADRB2 protein expression in old rats (Paulose and Balakrishnan, 2008). Thus, the ADRB2 upregulation observed at 6 h VOO ingestion could be promoted by the postprandial insulin peak.…”

Section: Discussionmentioning

confidence: 93%

Time Course of Changes in the Expression of Insulin Sensitivity-Related Genes after an Acute Load of Virgin Olive Oil

Konstantinidou

Khymenets

Covas

et al. 2009

OMICS: A Journal of Integrative Biology

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Our aim was to examine whether an acute fat load could induce changes in the expression of insulin sensitivity-related genes in human peripheral blood mononuclear cells. Selection of candidate genes was based on previous studies with sustained virgin olive oil (VOO) consumption and biological plausibility in relation to insulin sensitivity. Eleven healthy volunteers ingested raw VOO (50 mL). Blood samples were collected at 0, 1 and 6 h. Plasma glucose, insulin and hydroxytyrosol increased at 1 h and decreased at 6 h. Lipid oxidative damage increased at 6 h (p < 0.05). Gene expression changes were characterized based on quantification of the samples relative to a reference sample [i.e., relative quantification (RQ) method]. A 1 h downregulation was observed in O-linked-N-acetylglucosamine transferase (OGT, RQ: 0.62 +/- 0.32) and arachidonate-5-lipoxygenase-activating protein (ALOX5AP, RQ: 0.64 +/- 0.31) genes (p < 0.005). OGT was upregulated at 6 h (RQ: 1.88 +/- 0.28, p < 0.05). CD36 (thrombospondin receptor) was upregulated at 1 h (RQ: 1.6 +/- 0.8, p < 0.05) returning to the basal values at 6 h. Lipoic acid synthetase (LIAS), peroxisome proliferator-activated receptor binding protein (PPARBP), a disintegrin and metallopeptidase domain 17 (ADAM17), and adrenergic beta-2-receptor (ADRB2) genes were upregulated at 6 h (range for the mean RQ: 1.33-1.56) following an increasing linear trend (p < 0.05) from baseline to 6 h. ALOX5AP and OGT genes inversely correlated with insulin and glucose levels at 1 h. ADAM17 and ADRB2 inversely correlated with oxLDL at 6 h (p < 0.05). Taken together, these observations may inform the future clinical nutrigenomics study designs and indicate that a single dose of VOO can elicit quantifiable and rapid changes in gene expression in targets that are mechanistically relevant for insulin sensitivity and the metabolic syndrome.

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Section: Discussionmentioning

confidence: 93%

Time Course of Changes in the Expression of Insulin Sensitivity-Related Genes after an Acute Load of Virgin Olive Oil

Konstantinidou

Khymenets

Covas

et al. 2009

OMICS: A Journal of Integrative Biology

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“…Dopamine contents in the lysates of the hypothalamus were assayed by HPLC analysis according to Paulose and Balakrishnan [24]. The hypothalamus was homogenized in 0.4 N perchloric acid.…”

Section: Animals and Methodsmentioning

confidence: 99%

Central Prolactin Modulates Insulin Sensitivity and Insulin Secretion in Diabetic Rats

et al. 2012

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Background: Prolactin secretion is self-regulating as it acts upon hypothalamic dopaminergic systems which inhibit prolactin release from the anterior pitutary. Circulating prolactin improves glucose homeostasis by increasing insulin action and secretion, but central prolactin effects on glucose homeostasis have not been examined. Here, we determined that chronic central infusion of prolactin modulates insulin resistance and β-cell function and mass in 90% of pancreatectomized diabetic male rats. Methods: Diabetic rats were divided into three groups according to the dose of intracerebroventricular infusion of prolactin during 4 weeks: (1) low-dose prolactin (Low-PRL; 0.1 µg/h), (2) high-dose prolactin (High-PRL; 1 µg/h) and (3) vehicle only (cerebrospinal fluid). Nondiabetic rats were centrally infused with the vehicle. Results: Chronic intracerebroventricular infusion of Low-PRL lowered body weight and epididymal fat pads by increasing hypothalamic dopamine levels that reduced serum prolactin levels and potentiated leptin signaling. However, High-PRL slightly exacerbated energy dysregulation, decreased hypothalamic dopamine levels, and elevated serum prolactin levels. Both dosages promoted β-cell mass but in a different manner: Low-PRL decreased β-cell apoptosis, whereas High-PRL increased its proliferation. However, only Low-PRL enhanced first-phase insulin secretion and improved insulin sensitivity at a hyperglycemic state in comparison to the control. Low-PRL also increased glucose infusion rates and decreased hepatic glucose output in hyperinsulinemic states, signifying an improvement in hepatic insulin sensitivity. However, High-PRL exacerbated hepatic insulin resistance compared with the control diabetic rats. Conclusions: In contrast to the exacerbation of insulin resistance caused by High-PRL, Low-PRL may improve energy and glucose metabolism by increasing hypothalamic dopamine levels in diabetic rats.

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“…95% C.I. = −0.77, −0.01; p = 0.043; I 2 = 82.47%; based on 68 comparisons from 15 studies[62][63][64][65][66][67][68][69][70][71][72][73][74][75][76]) that was mirrored by an increase (Hedges'g = 0.87, 95% C.I. = 0.05, 1.69; p = 0.037; I 2 = 44.29%; based on two studies[77,78] fetching three comparisons) in brain insulinresistant animals.…”

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confidence: 99%

Insulin effects on core neurotransmitter pathways involved in schizophrenia neurobiology: a meta-analysis of preclinical studies. Implications for the treatment

et al. 2023

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Impairment of insulin action and metabolic dysregulation have traditionally been associated with schizophrenia, although the molecular basis of such association remains still elusive. The present meta-analysis aims to assess the impact of insulin action manipulations (i.e., hyperinsulinemia, hypoinsulinemia, systemic or brain insulin resistance) on glutamatergic, dopaminergic, γ-aminobutyric acid (GABA)ergic, and serotonergic pathways in the central nervous system. More than one hundred outcomes, including transcript or protein levels, kinetic parameters, and other components of the neurotransmitter pathways, were collected from cultured cells, animals, or humans, and meta-analyzed by applying a random-effects model and adopting Hedges’g to compare means. Two hundred fifteen studies met the inclusion criteria, of which 180 entered the quantitative synthesis. Significant impairments in key regulators of synaptic plasticity processes were detected as the result of insulin handlings. Specifically, protein levels of N-methyl-D-aspartate receptor (NMDAR) subunits including type 2A (NR2A) (Hedges’ g = −0.95, 95%C.I. = −1.50, −0.39; p = 0.001; I2 = 47.46%) and 2B (NR2B) (Hedges’g = −0.69, 95%C.I. = −1.35, −0.02; p = 0.043; I2 = 62.09%), and Postsynaptic density protein 95 (PSD-95) (Hedges’g = −0.91, 95%C.I. = −1.51, −0.32; p = 0.003; I2 = 77.81%) were found reduced in insulin-resistant animal models. Moreover, insulin-resistant animals showed significantly impaired dopamine transporter activity, whereas the dopamine D2 receptor mRNA expression (Hedges’g = 3.259; 95%C.I. = 0.497, 6.020; p = 0.021; I2 = 90.61%) increased under insulin deficiency conditions. Insulin action modulated glutamate and GABA release, as well as several enzymes involved in GABA and serotonin synthesis. These results suggest that brain neurotransmitter systems are susceptible to insulin signaling abnormalities, resembling the discrete psychotic disorders’ neurobiology and possibly contributing to the development of neurobiological hallmarks of treatment-resistant schizophrenia.

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Adrenergic, dopaminergic and serotonergic gene expression in low dose, long time insulin and somatotropin treatment to ageing rats: rejuvenation of brain function

Cited by 7 publications

References 40 publications

Time Course of Changes in the Expression of Insulin Sensitivity-Related Genes after an Acute Load of Virgin Olive Oil

Time Course of Changes in the Expression of Insulin Sensitivity-Related Genes after an Acute Load of Virgin Olive Oil

Central Prolactin Modulates Insulin Sensitivity and Insulin Secretion in Diabetic Rats

Insulin effects on core neurotransmitter pathways involved in schizophrenia neurobiology: a meta-analysis of preclinical studies. Implications for the treatment

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