Adsorption characteristics of cellulases from a constitutive mutant of Trichoderma reesei

Sethi, Benu; Mishra, Saroj; Bisaria, Virendra S.

doi:10.1016/s0922-338x(98)80119-0

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…Our adsorption on Avicel at 25 o C was 59.67 %, it was fallen to 52.94 % at 50 o C, which is also less than 10%. The most of researchers investigated adsorption of metal ions, dyes and enzymes on various lignocellulosic substrates at varying temperatures and found that by increasing temperature, the amount adsorbed increased but the time to reach equilibrium remains the same [Medve, 1994;Sethi et al, 1998;Kim et al, 2001;Qadeer, 2005;Alzaydien and Manasreh, 2009;Daoud et al, 2010;Du et al, 2014]. Our results are in agreement with their findings.…”

Section: Effect Of Temperature On Adsorption Of Cellulasessupporting

confidence: 90%

Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

Baig¹

2021

Preprint

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Desorption of active cellulases from lignocellulosic substrates is a potential technique to reuse cellulases for the production of bioethanol. For desorption studies, adsorption of cellulases had to be performed first. Adsorption of cellulases NS 50013 onto microcrystalline cellulose (Avicel PH 101) and wheat straw lignin (Protobind 1000) was studied. It was found that Protobind adsorbed twice the amount of cellulases than Avicel did. An adsorption strategy developed was to work at pH 5 and a temperature less than 323 K to get maximum adsorption on the cellulose component, less adsorption on the lignin component of lignocellulosic materials, and to harmonize adsorption temperature with the industrial hydrolysis situation. Desorption of cellulases from Avicel and Protobind over a range of 298 K to 343 K and a pH of 6 to 9 was studied. Desorption obtained at pH 9 and 333K was optimum for both Avicel and Protobind. Hence, desorption was enhanced by 21 % and 11% for Avicel and Protobind respectively. The cellulases activity for Avicel was 48 FPU mL-1 at pH 9, 333 K, 5% glycerol, representing 91 % of the initial activity and for Protobind, the activity was 33 FPU mL-1 which represents about 66 % of the initial activity. All of these values were higher than ever reported in literature. At pH 5 and 298 K the amount of cellulases desorbed from untreated wheat straw (WS) was 33 % of those initially used for the adsorption step. It was increased to 42 % when 30 % delignified WS was used, and was further increased to 48 % for 60 % delignified WS. Desorption obtained for 60 % delignified WS was 75 % at pH 9, 333K and 5% glycerol. The desorption strategy recommended for bioethanol producing industries, is: 1) removal of lignin; 2) adsorption of cellulases at pH 5 and lower than 323K; 3) hydrolysis of lignocellulosic material; and 4) desorption of cellulases from non-hydrolyzed material at 333 K, pH 9, with 5-10 % glycerol. The proposed strategic desorption of cellulases may reduce the cost of Canadian bioethanol production by 26.5 % due to 75 % recyclability of active cellulases.

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Section: Effect Of Temperature On Adsorption Of Cellulasessupporting

confidence: 90%

Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

Baig¹

2021

Preprint

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“…Kiriacou et al (1988) determined E max values ranging from 0.0047 to 0.0271 g cellulase/g cellulose using cellulase derived from T. reesei, which compare favorably with our own range from 0.0053 (untreated) to 0.0342 (acid-pretreated) g cellulase/g cellulose. Several other investigators have observed Langmuir adsorption behavior within this parameter range using Avicel as the substrate and cellulase from several strains of Trichoderma (Ooshima et al, 1983;Woodward et al, 1988;Stahlberg et al, 1991;Sethi et al, 1998).…”

Section: Discussionmentioning

confidence: 83%

Cellulase kinetics as a function of cellulose pretreatment

Bommarius

Katona

Cheben

et al. 2008

Metabolic Engineering

175

107

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“…Their findings were that by increasing temperature, equilibrium constant value was increased, but the time to reach equilibrium remains the same (Medve et al 1994;Sethi et al 1998;Kim et al 2000;Daoud et al 2010;Zheng et al 2013a;2014;Du et al 2014;Sukumaran et al 2017;Houston et al 2019). Some of the researchers have shown adsorption plot at one temperature and used the same equilibrium time for all other temperatures of studies (Sethi et al 1998;Houston et al 2019). Baig (2016a) submitted his observations about adsorption by stating at the start, adsorption progress with the time when the enzymes are placed with the substrate of interest.…”

Section: Time Of Contactmentioning

confidence: 99%

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

Baig

2020

Bioresour. Bioprocess.

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For the production of biofuel (bioethanol), enzymatic adsorption onto a lignocellulosic biomass surface is a prior condition for the enzymatic hydrolysis process to occur. Lignocellulosic substances are mainly composed of cellulose, hemicellulose and lignin. The polysaccharide matrix (cellulose and hemicellulose) is capable of producing bioethanol. Therefore, lignin is removed or its concentration is reduced from the adsorption substrates by pretreatments. Selected enzymes are used for the production of reducing sugars from cellulosic materials, which in turn are converted to bioethanol. Adsorption of enzymes onto the substrate surface is a complicated process. A large number of research have been performed on the adsorption process, but little has been done to understand the mechanism of adsorption process. This article reviews the mechanisms of adsorption of enzymes onto the biomass surfaces. A conceptual adsorption mechanism is presented which will fill the gaps in literature and help researchers and industry to use adsorption more efficiently. The process of enzymatic adsorption starts with the reciprocal interplay of enzymes and substrates and ends with the establishment of molecular and cellular binding. The kinetics of an enzymatic reaction is almost the same as that of a characteristic chemical catalytic reaction. The influencing factors discussed in detail are: surface characteristics of the participating materials, the environmental factors, such as the associated flow conditions, temperature, concentration, etc. Pretreatment of lignocellulosic materials and optimum range of shear force and temperature for getting better results of adsorption are recommended.

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Adsorption characteristics of cellulases from a constitutive mutant of Trichoderma reesei

Cited by 12 publications

References 15 publications

Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

Strategic adsorption/desorption of cellulases NS 50013 onto/from AVICEL PH 101 and protobind 1000

Cellulase kinetics as a function of cellulose pretreatment

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

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