Adsorption Chromatography: Mechanism and Materials

A Modular Approach for the Synthesis of Diverse Heterobifunctional Cyanine Dyes

“…Furthermore, due to the weakly acidic nature of silica gel, there is a tendency toward preferential adsorption of basic compounds relative to their adsorption on neutral and basic adsorbents. Here, as eluent, a mixture of DCM/MeOH proved optimal, as the adsorption of polar compounds is reduced in a polar medium . We tested the purification conditions for cyanine dye 1 .…”

“…Here, as eluent, a mixture of DCM/MeOH proved optimal, as the adsorption of polar compounds is reduced in a polar medium. 40 We tested the purification conditions for cyanine dye 1 . Impurities were removed by using a gradient of DCM/MeOH and the product was obtained by eluting with the addition of 1% triethylamine to the eluent mixture (a purity of >99% can be achieved for the cyanine dye 1 ; see Figure S.5 ).…”