Adsorption/desorption characteristics, separation and purification of phenazine‐1‐carboxylic acid from fermentation extract by macroporous adsorbing resins

Abstract

BACKGROUND Pseudomonas chlororaphis GP72 is a biocontrol strain with great capacity to produce a wide range of secondary metabolites including phenazine derivatives. Phenazine‐1‐carboxylic acid (PCA) is an environmentally friendly antibiotic with broad‐spectrum biological and pharmaceutical activities. Many studies have been focused on engineering high‐yielding strains and optimizing fermentation bioprocesses to enhance PCA biosynthesis. However, the separation and purification of PCA in biotechnological production remain to be addressed. In this context, the present work intended to develop a simple and proficient technique for the separation and purification of PCA from the fermentation broth of engineered P. chlororaphis GP72AN. RESULTS Five macroporous adsorbents with different physicochemical characteristics were screened to present their PCA separation potential. Among them, HP‐20 resin was found highly suitable based on its promising adsorption and desorption performance in a static batch process. Langmuir and Freundlich isotherms deciphered the solute binding affinity towards HP‐20 adsorbent at varying temperatures, and the experimental data fitted best to the Langmuir isotherm model. The column‐assisted dynamic adsorption–desorption trials were executed to optimize the functional parameters for PCA separation. Under standardized separating conditions, the average adsorption capacity and desorption ratio of PCA were 24.4 ± 1.5 mg g−1 dry resin and 86.5 ± 1.3%, respectively. After treatment with HP‐20 resin, PCA contents in the product were considerably increased with a recovery yield of 78.4 ± 2.4%. CONCLUSION In conclusion, the method proposed herein achieved effective separation and purification of PCA by applying HP‐20 resin. The results obtained also suggest an encouraging basis for scale‐up preparation of PCA and other high‐value phenazine compounds of industrial interests. © 2018 Society of Chemical Industry

Simultaneous Purification and Separation of Syringoside and Oleuropein from Syringa oblata Lindl. Extract Using Macroporous Resin

“…HPD-100B is a nonpolar resin (Table 1), and the two targeted components are low polarity compounds. is is consistent with the results of Bilal et al [33], who reported that nonpolar compounds could easily be adsorbed by nonpolar resins. Compared with syringoside, oleuropein has less polarity.…”

Recent Developments in the Biological Activities, Bioproduction, and Applications of Pseudomonas spp. Phenazines

“…Organic solvents such as ethyl acetate, dichloromethane, chloroform, methylene dichloride, toluene, or diethyl ether are the most reported for this step of the purification process. Then, the organic phase where phenazines are dissolved can be evaporated and the dry solids dissolved in acetonitrile or methanol [ 19 , 20 , 22 , 23 , 27 , 29 , 32 , 52 , 68 , 69 , 70 ]. Some authors, after solvent utilization, include a chromatography step with silica gel to enhance the purification [ 29 , 71 , 72 , 73 , 74 ].…”

“…The separation and purification are mostly reported by thin layer chromatography (TLC) and preparative high-performance liquid chromatography (HPLC) which have good separation resolutions [ 68 , 71 , 75 , 76 , 77 ]. However, other techniques are being studied, such as capillary electrophoresis, free flow electrophoresis, and the use of microporous adsorbent resins as alternatives to reduce the price, the use of organic solvents and the time required for the separation [ 68 , 70 , 78 , 79 , 80 ]. Liu et al [ 78 ] applied capillary zone electrophoresis to separate and quantify PCA and 2-OH-PHZ from the culture broth of P. chlororaphis GP72, after an extraction step, offering a rapid and sensitive detection.…”

In situ solid-liquid extraction enhances recovery of taxadiene from engineered S. cerevisiae cell factories

“…The 50 % taxadiene repartition between biomass and resin beads provides an important piece of information, as it showcases where the taxadiene that is being detected in the system is being captured. To our knowledge, this information has not been commonly reported as the extraction is generally made to the biomass and resin in conjunction [16,29,46] or to the single beads without biomass [17,47]. These results could have an important impact into determining the capacity of the resin beads to relieve the cells from intracellular toxicity of selected NP among other phenomena.…”