Adsorption of chain type–specific ABO antibodies on Sepharose‐linked A and B tetrasaccharides

Abstract

There are chain type-specific anti-A and anti-B IgG, and an adsorber based on a combination of Types 1 through 4 A or B tetrasaccharides will be a more efficient adsorber than an adsorber based on the A or B trisaccharides.

Molecular Deciphering of the ABO System as a Basis for Novel Diagnostics and Therapeutics in ABO Incompatible Transplantation

“…In some cases, anti-ABO Ab titers cannot be reduced enough using specific IA columns to allow a safe ABOi transplantation even if the number of IA sessions are increased. If this is due to an inability of antigen-specific IA columns carrying the A and B trisaccharides to remove chain-type specific Abs (discussed below) remains to be shown [49,133,134]. An alternative explanation may be that the synthesis rate by anti-A and anti-B producing plasma cells in some patients keep up with the pace of Ab removal.…”

“…In some cases, IA columns carrying A or B trisaccharides will not reduce the titers sufficiently to allow transplantation [133,158]. One potential explanation for this may be that subpopulations of anti-A and anti-B require the fourth sugar in the carbohydrate chain for binding, and will therefore not bind the A or B trisaccharide determinant with sufficient avidity [133,158]. Such Abs are not removed by Biosynsorb [131][132][133]158] or Glycosorb R -ABO [158].…”

“…It is likely that the continuous synthesis of anti-A and anti-B also contributes to the overnight rebound of plasma Abs. In some cases, IA columns carrying A or B trisaccharides will not reduce the titers sufficiently to allow transplantation [133,158]. One potential explanation for this may be that subpopulations of anti-A and anti-B require the fourth sugar in the carbohydrate chain for binding, and will therefore not bind the A or B trisaccharide determinant with sufficient avidity [133,158].…”

“…In some patients, anti-A Abs were induced that required the A determinant to be presented on a particular outer core saccharide chain in order to bind [130,131]. Healthy blood donors were recently also shown to have chain type-specific A and B Abs in their serum [132,133]. Furthermore, mouse monoclonal antibodies (mAbs) specific for the different A antigens have been generated [78], suggesting that the human and mouse antibody response can distinguish between structurally distinct A and B antigens.…”

Universal Blood Products

“…Özellikle lojistik kolaylıkları (derin dondurucu gereksiniminın ortadan kalkması, uzak noktalara taşıma kolaylığı, kırılgan donmuş torbanın çatla-ması sorunun olmaması, ağırlık azalması gibi) ile dikkati çekmektedir. Ayrıca "anti-A" ve "anti-B" antikorlarının elimine edilmesini hedefleyen birçok çalışma ve kullanıma sokulmuş ürünler mevcuttur (9,(17)(18)(19)(20)(21) . Bunlardan birisi çifte filtreli plazmaferez uygulamasıdır.…”