Adsorption of ochratoxin A and zearalenone by potential probiotic Saccharomyces cerevisiae strains and its relation with cell wall thickness

Abstract

Aims: To examine Saccharomyces cerevisae strains with previously reported beneficial properties and aflatoxin B1 binding capacity, for their ability to remove ochratoxin A (OTA) and zearalenone (ZEA) and to study the relation between cell wall thickness and detoxificant ability of yeast strains. Methods and Results: A mycotoxin binding assay at different toxin concentrations and the effect of gastrointestinal conditions on mycotoxin binding were evaluated. Ultrastructural studies of yeast cells were carried out with transmission electronic microscopy. All tested strains were capable of removing OTA and ZEA. Saccharomyces cerevisiae RC012 and RC016 showed the highest OTA removal percentage, whereas RC009 and RC012 strains showed the highest ZEA removal percentages. The cell diameter/cell wall thickness relation showed a correlation between cell wall amount and mycotoxin removal ability. After exposure to gastrointestinal conditions, a significant increase in mycotoxin binding was observed. Conclusions: All tested Saccharomyces cerevisiae strains were able to remove OTA and ZEA, and physical adsorption would be the main mechanism involved in ochratoxin A and ZEA removal. Gastrointestinal conditions would enhance adsorption and not decrease mycotoxin–adsorbent interactions. Significance and Impact of the Study: Live strains with mycotoxin binding ability and beneficial properties are potential probiotics that could be included in animal feed. Previous and present results suggest that the RC008 and RC016 strains are very promising candidates for functional feed product development.

Stability of Ochratoxin a During Bread Making Process

“…Armando et al . () were showed that Saccharomyces cerevisiae RC012 and RC016 strains in vitro conditions had the highest OTA adsorption percentages and the cell diameter/cell wall thickness relation revealed a correlation between cell wall amount and mycotoxin elimination stability. A yeast‐based product was reported to adsorb up to 68% ZEA, 29% AFB 1 and 62% OTA (Joannis‐Cassan et al .…”

Statistical optimization of culture conditions for biomass production of probiotic gut-borneSaccharomyces cerevisiaestrain able to reduce fumonisin B1

“…The union of binder probiotics with toxins reduces their availability and, consequently, the absorption of the toxin in the gastrointestinal tract. Reduction in other major mycotoxins, aflatoxin B 1 , zearalenone and ochratoxin A, by the probiotic S. cerevisiae RC016 strain has also been shown in vitro (Armando et al , 2012. In the absence of a simple detoxification method for foods and feeds contaminated by FB 1 , the use of yeast selected strains appears as a promising approach to reduce their toxicological effects.…”

In vivo stability of the complex ochratoxin A – Saccharomyces cerevisiae starter strains

“…for aflatoxin bound by lactobacilli, and enterobacteria (Fazeli et al, 2009;Serrano-Niño et al, 2013) or for OTA bound by wine lactic acid bacteria (Del Prete et al, 2007). Concerning the strong effect of the kind of the strain, some possible ideas could be: (i) the total charge and its distribution, the size of the pores and the surface area (Huwig, Freimund, Kappeli, & Dutler, 2001), but also (ii) dissimilar cell diameter⁄ cell wall thickness relation (Armando et al, 2012) or (iii) differences in the composition of cell wall: e.g. a high chitin content decreased the flexibility of cell wall with a consequent lower capacity to form complexes with zearalenone in S. cerevisiae (Yiannikouris et al, 2004).…”