Adtrp regulates thermogenic activity of adipose tissue via mediating the secretion of S100b

Song, Runjie; Du, Yaqi; Liu, Huijiao; Li, Xiangdong

doi:10.1007/s00018-022-04441-9

Cited by 7 publications

(7 citation statements)

References 59 publications

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“… 56 Another factor regulating the thermogenic β3-AR signaling cascade is Adtrp ; Adtrp -deficient mice are cold-intolerant and have defective thermogenesis. 57 Thus, cold-activated induction specifically of the longer (enzymatically active) Adtrp isoform shown here might enhance BAT function. Peroxisome derived lipids are required for brown fat-mediated thermogenesis through regulation of cold-induced mitochondrial fission.…”

Section: Discussionmentioning

confidence: 75%

Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome

Engelhard¹,

Khani²,

Derdak³

et al. 2023

iScience

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Section: Discussionmentioning

confidence: 75%

Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome

Engelhard¹,

Khani²,

Derdak³

et al. 2023

iScience

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“…We found little overlap between stringtie and FLAIR reannoations in line with other reports (Ringeling et al, 2022). Among the genes with significant isoform switches between cold-activated BAT compared to the controls, we observed phosphodiesterase 4D ( Pde4 d), regulating levels of the signalling intermediate cAMP, which activates lipolysis, glucose uptake, and thermogenesis in brown adipocytes (Reverte-Salisa et al, 2019); the thermogenesis regulating hydrolase androgen dependent TFPI regulating protein ( Adtrp; P. Li et al, 2022) and regulators of fatty acid metabolism (acyl-CoA synthetase long-chain family member 5; Acsl5 ), glycolysis (Aldolase A; Aldoa ), protein sorting (endoplasmic reticulum-golgi intermediate compartment 1; Ergic 1), lipid synthesis (MLX interacting protein-like; Mlxip l), beta-oxidation (sterol carrier protein 2, liver; Scp2 ) and protein cysteinylation (cysteinyl-tRNA synthetase gene; Cars2; Akaike et al, 2017; Fig. 7C-E and S7).…”

Section: Resultsmentioning

confidence: 99%

“…Cold-activated induction of Pde4d -long might lead to reduced thermogenic cAMP signalling since Pde4d -long controls cAMP levels negatively in a spatial manner due to UCR motifs present in the long Pde4d isoform (Lynch et al, 2007). Another factor regulating the thermogenic β3-AR signalling cascade is Adtrp ; Adtrp -deficient mice are cold-intolerant and have defective thermogenesis (P. Li et al, 2022). Thus, cold-activated induction specifically of the longer (enzymatically active) Adtrp isoform shown here might enhance BAT function.…”

Section: Discussionmentioning

confidence: 99%

Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome

Engelhard

Khani

Derdak

et al. 2022

Preprint

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Alternative transcription increases transcriptome complexity by expression of multiple transcripts per gene and thus fine tunes cellular identity and function. Annotation and quantification of transcripts at complex loci using short-read sequencing is non-trivial. Recent long-read sequencing methods such as those from Oxford Nanopore Technologies (ONT) and Pacific Biosciences aim at overcoming these problems by sequencing full length transcripts. Activation of BAT thermogenesis involves major transcriptomic remodelling and positively affects metabolism via increased energy expenditure and endocrine factors. Here we comprehensively benchmark features of ONT long-read sequencing protocols compared to Illumina shortread sequencing assessing alignment characteristics, gene and transcript detection and quantification, differential gene and transcript expression, transcriptome reannotation and differential transcript usage (DTU). We find that ONT sequencing is superior to Illumina for transcriptome reassembly and reduces the risk of false-positive events due to the ability to unambiguously map reads to transcripts, at the expense of statistical power for calling differentially expressed features. We identified novel isoforms of genes undergoing DTU in cold-activated BAT including Cars2, Adtrp, Acsl5, Scp2, Aldoa and Pde4d, validated by RT-qPCR. Finally, we provide a reannotation of the murine iBAT transcriptome as a valuable resource for researchers interested in the molecular biology underlying the regulation of BAT.

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“…The S100B, which is expressed in adipose tissue [15,16], has been associated with the pathophysiology of obesity-promoting macrophage-based inflammation [17,50]. The serum level of S100B correlates with insulin resistance, metabolic risk score, and fat cell size [17].…”

Section: Discussionmentioning

confidence: 99%

“…The S100B is a calcium-binding protein responsible for transcriptional regulation and DNA repair, cell differentiation, cell growth and migration, and programmed cell death [12]. The S100B is predominantly expressed by astrocytes [13], and also by other cell types: melanocytes [14], chondrocytes, adipocytes [15,16], skeletal muscle, and a few other cell types [17]. In their research, Fujiya et al [18] proposed that the S100B functions as an adipokine in the interaction between adipocytes and macrophages.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Syndrome and Adipokines Profile in Bipolar Depression

Bilska,

Dmitrzak-Węglarz,

Osip

et al. 2023

Nutrients

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Metabolic syndrome (MS) is a growing social, economic, and health problem. MS coexists with nearly half of all patients with affective disorders. This study aimed to evaluate the neurobiological parameters (clinical, anthropometric, biochemical, adipokines levels, and ultrasound of carotid arteries) and their relationship with the development of MS in patients with bipolar disorder. The study group consisted of 70 patients (50 women and 20 men) hospitalized due to episodes of depression in the course of bipolar disorders. The Hamilton Depression Rating Scale was used to assess the severity of the depression symptoms in an acute state of illness and after six weeks of treatment. The serum concentration of adipokines was determined using an ELISA method. The main finding of this study is that the following adipokines correlated with MS in the bipolar depression women group: visfatin, S100B, and leptin had a positive correlation, whereas adiponectin, leptin-receptor, and adiponectin/leptin ratio showed a negative correlation. Moreover, the adiponectin/leptin ratio showed moderate to strong negative correlation with insulin level, BMI, waist circumference, triglyceride level, treatment with metformin, and a positive moderate correlation with HDL. The adiponectin/leptin ratio may be an effective tool to assess MS in depressed female bipolar patients.

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Adtrp regulates thermogenic activity of adipose tissue via mediating the secretion of S100b

Cited by 7 publications

References 59 publications

Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome

Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome

Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome

Metabolic Syndrome and Adipokines Profile in Bipolar Depression

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