Advance methods for the qualitative and quantitative determination of microorganisms

Ayhan, Kamuran; Coşansu, Serap; Orhan-Yanıkan, Esin; Gülseren, Gülcihan

doi:10.1016/j.microc.2021.106188

Cited by 15 publications

(5 citation statements)

References 208 publications

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“…Most commercially available biosensors are based on electrochemical technologies, which require complex conducting lines and electronic components. Other optical biosensors (which are commonly used alongside electrochemical biosensors) detect changes in ultraviolet and visible absorption, fluorescence, phosphorescence, bioluminescence, chemiluminescence, reflectance, scattering, and refractive index . The changes in optical properties are caused by the interaction of the biocatalyst with the target analyte. , Recently, photonic color has been used as a detection property for optical biosensors.…”

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confidence: 99%

Bilayer Actuator Film for Urea Biosensing with Dual Responsiveness: Bending Actuation and Photonic Color Change

Adane

Park

2023

ACS Sens.

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A noninvasive sweat-based biosensor was developed for urea detection using a photonic bilayer actuator film (BAF) consisting of an interpenetrating polymer network (IPN) as the active layer and a flexible poly(ethylene terephthalate) (PET) substrate as the passive layer (IPN/ PET). The active IPN layer comprises intertwined solid-state cholesteric liquid crystal and poly(acrylic acid) (PAA) networks. Urease was immobilized in the PAA network in the IPN layer of the photonic BAF. The interaction with aqueous urea altered the curvature and photonic color of the photonic urease-immobilized IPN/PET (IPN urease /PET) BAF. The curvature (and wavelength of the photonic color) of the IPN urease /PET BAF increased linearly with urea concentration (C urea ) in the range of C urea = 20− 65 (and 30−65) mM with a limit of detection value of 1.42 (and 1.34) mM. The developed photonic IPN urease /PET BAF exhibited high selectivity toward urea and excellent spike test results with real human sweat. This novel IPN urease /PET BAF is promising because it enables battery-free, cost-effective, and visual detection-based analysis without the use of sophisticated instruments. Furthermore, the application of this photonic IPN/PET BAF can be easily extended to other biosensors by immobilizing other receptors on the IPN.

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confidence: 99%

Bilayer Actuator Film for Urea Biosensing with Dual Responsiveness: Bending Actuation and Photonic Color Change

Adane

Park

2023

ACS Sens.

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“…MALDI-TOF-MS was used to accurately and rapidly identify the five high-risk clones of P. aeruginosa sequence ST111, ST175, ST235, ST253, and ST395, also was applied in P. aeruginosa drug resistance analysis such as carbapenemase ( Mulet et al, 2021 ). MALDI-TOF-MS exhibits limited resolving power, and therefore does not supply sequence-based ID necessarily; microbial ID using MALDI-TOF-MS is based on spectral fingerprint patterns rather than the identity of each spectral peak ( Ayhan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis

Wang

Ai-ming

et al. 2022

Front. Microbiol.

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Mining novel specific molecular targets and establishing efficient identification methods are significant for detecting Pseudomonas aeruginosa, which can enable P. aeruginosa tracing in food and water. Pangenome analysis was used to analyze the whole genomic sequences of 2017 strains (including 1,000 P. aeruginosa strains and 1,017 other common foodborne pathogen strains) downloaded from gene databases to obtain novel species-specific genes, yielding a total of 11 such genes. Four novel target genes, UCBPP-PA14_00095, UCBPP-PA14_03237, UCBPP-PA14_04976, and UCBPP-PA14_03627, were selected for use, which had 100% coverage in the target strain and were not present in nontarget bacteria. PCR primers (PA1, PA2, PA3, and PA4) and qPCR primers (PA12, PA13, PA14, and PA15) were designed based on these target genes to establish detection methods. For the PCR primer set, the minimum detection limit for DNA was 65.4 fg/μl, which was observed for primer set PA2 of the UCBPP-PA14_03237 gene. The detection limit in pure culture without pre-enrichment was 105 colony-forming units (CFU)/ml for primer set PA1, 103 CFU/ml for primer set PA2, and 104 CFU/ml for primer set PA3 and primer set PA4. Then, qPCR standard curves were established based on the novel species-specific targets. The standard curves showed perfect linear correlations, with R2 values of 0.9901 for primer set PA12, 0.9915 for primer set PA13, 0.9924 for primer set PA14, and 0.9935 for primer set PA15. The minimum detection limit of the real-time PCR (qPCR) assay was 102 CFU/ml for pure cultures of P. aeruginosa. Compared with the endpoint PCR and traditional culture methods, the qPCR assay was more sensitive by one or two orders of magnitude. The feasibility of these methods was satisfactory in terms of sensitivity, specificity, and efficiency after evaluating 29 ready-to-eat vegetable samples and was almost consistent with that of the national standard detection method. The developed assays can be applied for rapid screening and detection of pathogenic P. aeruginosa, providing accurate results to inform effective monitoring measures in order to improve microbiological safety.

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“…Carbohydrate speci c spectral regions of four selected samples were compared to elucidate the relationship between the isolation method and the quality of the product. In most cases, improvement in the yield quantity results in a decrease in quality [25,26]. Therefore, two high yield and two low yield extracts were selected for comparison.…”

Section: Fourier Transform Infrared Spectrophotometer (Ftir) Analysismentioning

confidence: 99%

Analytical Optimization of Extraction Parameters to Produce High-Yield Low Methoxy Pectin with Significant Bioactivity

Uslu

Yeniterzi

Narin

et al. 2022

Preprint

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The pectin isolation technique from sugar beet pulp was optimized to acquire high-quality bioactive pectin with the highest yield and low esterification degree. The influence of each isolation parameter on the success of pectin production was investigated by analytical characterization methods. With this step-by-step analytic investigation, we could be able to achieve ~ 45% yield with a ~ 10% low esterification degree. In addition to compositional quality, bioactivity and anticancer profile of the obtained sugar beet pulp pectin (SBPP) was investigated on SaOS-2 osteosarcoma cell line. In comparison with citrus pectin (CP), which is frequently applied pectin derivative as an anticancer agent, SBPP revealed prolonged antiproliferative profile for cancer cells. In addition to proliferation, intracellular reactive oxygen species (ROS) level and Bcl-2 anti-apoptotic gene expression were surveyed in vitro. Interestingly, the lower concentration of SBPP (0.25%) induced a significant rise in intracellular ROS level and anti-apoptotic gene Bcl-2 expression on the contrary to CP. All results demonstrated that different pectin types can lead different biological mechanism of action and the isolated SBPP by using optimized protocol exhibited great potential for biomedical applications without requirement of additional post-modification after isolation.

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Advance methods for the qualitative and quantitative determination of microorganisms

Cited by 15 publications

References 208 publications

Bilayer Actuator Film for Urea Biosensing with Dual Responsiveness: Bending Actuation and Photonic Color Change

Bilayer Actuator Film for Urea Biosensing with Dual Responsiveness: Bending Actuation and Photonic Color Change

Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis

Analytical Optimization of Extraction Parameters to Produce High-Yield Low Methoxy Pectin with Significant Bioactivity

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