Advanced adipose-derived stem cell protein extracts with antioxidant activity modulates matrix metalloproteinases in human dermal fibroblasts

Chae, Younbyoung; Lee, Jae Seol; Park, Hye-Jung; Park, In-Hwan; Kim, Moon‐Moo; Park, Ye-Hyoung; Kim, Dong-Soo; Lee, Jong‐Hwan

doi:10.1016/j.etap.2012.04.010

Cited by 12 publications

(8 citation statements)

References 25 publications

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“…The results showed that ADSCs efficiently reduced the lipid peroxidation process via elevating the expression of superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) levels in the liver [25]. Many studies also indicate the anti-oxidization effect of human ADSC transplantation which is consistent with the reduction of ALT indexes in our study [41][42][43]. In this study, the total number of circulating leukocytes was considered as an indication for immune cell mobilization.…”

Section: Discussionsupporting

confidence: 81%

Human adipose-derived stem cells pre-treated with platelet-rich plasma and hepatocyte growth factor alleviate liver fibrosis in mice

Nguyen

Lê

et al. 2018

Biomed. Res. Ther.

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Background: Transplantation of adipose-derived stem cells (ADSCs) is a potential therapy for a variety of liver diseases. Previous studies have shown that ADSC-based therapy is promising for liver fibrosis treatment. Recently, many publications have suggested that pretreating ADSCs with growth factors before transplantation can elevate the effectiveness of the therapy. Therefore, we hypothesize that human ADSCs (hADSCs) pretreated with platelet-rich plasma (PRP) and hepatocyte growth factor (HGF), compared to ADSCs alone, would accelerate the treatment effects on liver fibrosis in mice. Methods: The hADSCs were cultured solely with conventional media, or with HGF (human recombinant; 20 ng/ml), or with HGF and PRP (from healthy human blood, 10%), concomitantly added to the medium for 7 days before transplantation. Eight-week-old male mice were treated with CCl4 (1 ml/kg) for 11 weeks to induce liver fibrosis. The mice were then subsequently divided into: 1) Placebo group (PBS injection); 2) ADSCs/HGF+PRP (5x105HGF and PRP pre-treated cells/mice); 3) ADSCs/HGF (5x105HGF pre-treated cells/mice) and 4) ADSCs (5x105non-pretreated cells/mice). Results: Seven days post-transplantation, the alanine transaminase (ALT) level in the placebo was notably elevated (143.10±14.96 IU/L), compared to ALT levels of ADSCs-, ADSCs/HGF+PR-, and ADSCs/HGF-transplanted mice, which showed an improvement (67.94±18.57 IU/L, 49.44±7.56 IU/L, and 57.93±5.75 IU/L, respectively). The procollagen-α1 and alpha-smooth muscle actin (α-SMA) mRNA levels were significantly down-regulated in the ADSCs-transplanted group compared to those of the placebo. Importantly, these levels were lower in ADSCs/HGF+PR (procollagen-α1: 75.64±45.89; α-SMA: 36.17±36.09) than those of ADSCs/HGF (procollagen-α1: 212.8±84.35; α-SMA: 52.41±7.93) and ADSCs only (procollagen-α1: 310.50 ± 55.36; α-SMA: 184.70±14.06). Stem cell transplantation also improved histological index, reducing inflammation and collagen/necrotic structure accumulation. However, there were no statistical differences between three ADSCs treatment groups after 14 days after transplantation. Conclusion: Pre-treatment with PRP and HGF for 7 days enhanced the efficacy of ADSCs in alleviating liver fibrosis in vivo.

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Section: Discussionsupporting

confidence: 81%

Human adipose-derived stem cells pre-treated with platelet-rich plasma and hepatocyte growth factor alleviate liver fibrosis in mice

Nguyen

Lê

et al. 2018

Biomed. Res. Ther.

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“…Collagen precursors are produced by fibroblasts in skin and play an important role in maintaining collagen structure [ 22 ]. To examine whether MHY498 has protective effect on a collagen precursor in fibroblasts after UVB exposure, we measured intracellular type 1 procollagen (a major collagen precursor in skin) concentration in human dermal fibroblasts cultured primarily from human fetal skin [ 23 ] using an ELISA kit. UVB exposure significantly decreased type 1 procollagen concentration in the fibroblasts ( Figure 4(c) ).…”

Section: Resultsmentioning

confidence: 99%

(Z)‐5‐(2,4‐Dihydroxybenzylidene)thiazolidine‐2,4‐dione Prevents UVB‐Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM‐2 Hairless Mice

Lee

Moon

Kim

et al. 2016

Oxidative Medicine and Cellular Longevity

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Background. Uncontrolled melanogenesis and wrinkle formation are an indication of photoaging. Our previous studies demonstrated that (Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione (MHY498) inhibited tyrosinase activity and melanogenesis in vitro. Objective. To examine in vivo effects of MHY498 as an antiaging compound on UVB-induced melanogenesis and wrinkle formation, we topically applied MHY498 on dorsal skin of HRM-2 hairless mice. Methods. Using histological analysis, we evaluated effects of MHY498 on melanogenesis and wrinkle formation after UVB exposure. In addition, related molecular signaling pathways were examined using western blotting, fluorometric assay, and enzyme-linked immunosorbent assay. Results. MHY498 suppressed UVB-induced melanogenesis by inhibiting phosphorylation of CREB and translocation of MITF protein into the nucleus, which are key factors for tyrosinase expression. Consistently, tyrosinase protein levels were notably reduced in the dorsal skin of the hairless mice by MHY498 treatment. Furthermore, MHY498 inhibited UVB-induced wrinkle formation and collagen fiber destruction by increasing type 1 procollagen concentration and decreasing protein expression levels of MMPs, which play an essential role in collagen fiber degradation. As a mechanism, MHY498 notably ameliorated UVB-induced oxidative stress and NF-κB activation in the dermal skin of the hairless mice. Conclusion. Our study suggests that MHY498 can be used as a therapeutic or cosmetic agent for preventing uncontrolled melanogenesis and wrinkle formation.

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“…The use of embryonic stem cell therapy for clinical application has been limited as a result of potential tumorigenicity and ethical opposition (Taha & Hedayati, ). However, adult mesenchymal stem cells (MSCs) have aroused significant interest, as they exhibit multi‐potentiality and can differentiate into specific cell types, with the possibility of lifelong organ regeneration (Chae et al ., ). Bone marrow–derived MSCs have been shown to prevent pulmonary and renal fibrosis, permit recovery of related organ function and ameliorate changes in liver function tests in experimental fibrosis models (Ortiz et al ., ; Sakaida et al ., ; Alfarano et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Adipose tissue-derived stem cell therapy for prevention and treatment of erectile dysfunction in a rat model of Peyronie's disease

et al. 2014

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SUMMARYPeyronie's disease (PD) is a localized connective tissue disorder that involves the tunica albuginea (TA) of the penis. While surgical correction remains the gold standard, the search for an effective and less invasive therapy continues. The objective of this study was to evaluate the effects of intratunical injection of adipose tissue-derived stem cells (ADSCs) for the prevention and treatment of erectile dysfunction in a rat model of PD. Twenty-four male Sprague-Dawley rats (300-350 g) were randomly divided into four groups: sham, PD, PD + ADSC (prevention) and PD + ADSC (treatment). All rats underwent penile injections into the TA with 50 lL vehicle (sham) or 0.5 lg transforming growth factor (TGF)-b1 (remaining groups). The ADSC groups received intratunical injections with 0.5 million rat-labelled ADSCs on day 0 (prevention) or day 30 (treatment). Forty-five days following TGF-b1 injection, rats underwent cavernous nerve stimulation (CNS) with total intracavernous-to-mean arterial pressure ratio (ICP/MAP) and total ICP recorded to measure response to therapy. Tissues were evaluated histologically and for mRNA expression of tissue inhibitors of metalloproteinases (TIMPs), matrix metalloproteinases (MMPs) and zymographic activity of MMPs. Statistical analysis was performed by analysis of variance followed by the Tukey test for post hoc comparisons. In both prevention and treatment groups, intratunical injection of ADSCs resulted in significantly higher ICP/MAP and total ICP in response to CNS compared with the PD group. Local injection of ADSCs prevented and/or reduced Peyronie's-like changes by decreasing the expression of TIMPs, and stimulating expression and activity of MMPs. This study documents the preventive and therapeutic benefits of ADSC on penile fibrosis and erectile function in an animal model of PD.

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Advanced adipose-derived stem cell protein extracts with antioxidant activity modulates matrix metalloproteinases in human dermal fibroblasts

Cited by 12 publications

References 25 publications

Human adipose-derived stem cells pre-treated with platelet-rich plasma and hepatocyte growth factor alleviate liver fibrosis in mice

Human adipose-derived stem cells pre-treated with platelet-rich plasma and hepatocyte growth factor alleviate liver fibrosis in mice

(Z)‐5‐(2,4‐Dihydroxybenzylidene)thiazolidine‐2,4‐dione Prevents UVB‐Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM‐2 Hairless Mice

Adipose tissue-derived stem cell therapy for prevention and treatment of erectile dysfunction in a rat model of Peyronie's disease

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