Advanced Amplitude Centering as an Invigorating Manipulation for Unified Wavelength Spectral Resolution of Ternary Mixtures

Abstract

Objective: This work presented the novel spectrophotometric approach namely, advanced amplitude centering (AAC). The method was applied for the resolution of ternary mixtures with partially or completely overlapped spectra.Methods: Advanced amplitude centering was based on the determination of ternary mixtures using single divisor where the concentrations of the components are determined through progressive manipulation performed on the same ratio spectrum. The centered amplitude at unified wavelength was resolved and applied for the determination of three components with partially and severely overlapped spectra. The work discussed the applications and advantages of the novel univariate advanced amplitude centering compared to the chemometric model, partial least square (PLS).Results: The specificity of the proposed methods was checked using laboratory-prepared mixtures of amlodipine (AML), valsartan (VAL) and hydrochlorothiazide (HCT) and was successfully applied for the analysis of two pharmaceutical formulations. The validity of results was assessed by applying the standard addition technique.Conclusion: The results obtained were found to agree statistically with those obtained by a reported method, showing no significant difference with respect to accuracy and precision.

Spider diagram and sustainability evaluation of UV-methods strategy for quantification of aspirin and sildenafil citrate in the presence of salicylic acid in their bulk and formulation

“…In this paper, ASA and SIC were quantified synchronously in the presence of SA by Dual Wavelength in Ratio Spectra (DW-RS) [ 32 , 33 ] which rely on choosing two wavelengths in which the intervention component has the same absorptivities. In addition, Advanced Amplitude Centering (AAC) [ 34 , 35 ] was employed for the synchronous quantification utilizing a single divisor; supplying a favorable way to educe the target component without any interference from the escorting components in their triplex commixture. Moreover, the Double Divisor of the Ratio Difference Derivative (DDRD-D 1 ) [ 36 , 37 ] was assigned, supplying an exceptional way to elicit the signal of the target component without any interference from the escorting components in their triplex commixture.…”

Green Method for Acetaminophen and Ibuprofen Simultaneous Assay in the Combination Tablet Using Ftir

“…The method arranged to follow the previous research development of the direct assay using FTIR using derivated absorbance spectra has been reported previously [21,22]. The derivative spectral has been commonly used in other spectrometry, such as uv-visible and fluorometry [18][19][20]. Standards of ACT and IBU are respectively mixed with KBr in a varied ratio, beaten into pellets at pressure 10-20 Mpa, and measured the spectrum.…”

“…To enhance the specificity, all the absorbance spectrums were derivated [18][19][20][21][22]. The result is shown in fig.…”

“…In the previous researches, we have tried to develop the quantitative method using derivative mathematically. This technique has been frequently used to separate the overlaid spectra in other spectroscopy methods, such as uv-visible as well as spectrofluorometry [18][19][20]. Using the derivatisation, the lack of specificity can overcome successfully.…”

Utilization of absorbance subtraction and ratio difference green spectrophotometric methods for the quantification of alfuzosin hydrochloride and tadalafil in their binary mixture

“…The goal of this research was to provide the first spectrophotometric methods for determining ALF and TAD in their combination. The developed methods were absorbance subtraction [ 14 ] and ratio difference [ 15 , 16 ]. The absorbance subtraction method simply manipulates the zero absorption spectra using an isoabsorptive point (λ iso ) and an absorbance factor, whereas the ratio difference method manipulates the ratio spectra.…”