Advanced approach to activity evaluation for released-active forms of antibodies to interferon-gamma by enzyme-linked immunoassay

Don, Elena; Bobrovnik, S. A.; Sherriff, Gordon; Myslivets, A. A.; Тарасов, С. А.; Epstein, Oleg

doi:10.1080/15321819.2019.1567536

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…A number of publications have recently appeared on the biological activity of extremely diluted solutions of antibodies to IFNγ [10][11][12][13]. It was shown that this activity is based on the ability of extremely diluted solutions of antibodies to modify the conformation of their target molecule, IFNγ.…”

Section: Introductionmentioning

confidence: 99%

Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution

Penkov

2021

Pharmaceutics

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Terahertz spectroscopy allows for the analysis of vibrations corresponding to the large-scale structural movements and collective dynamics of hydrogen-bonded water molecules. Previously, differences had been detected in the emission spectra of interferon-gamma (IFNγ) solutions surrounded by extremely diluted solutions of either IFNγ or antibodies to IFNγ without direct contact compared to a control. Here we aimed to analyse the structural properties of water in a sample of an aqueous solution of IFNγ via terahertz time-domain spectroscopy (THz-TDS). Tubes with the IFNγ solution were immersed in fluidised lactose saturated with test samples (dilutions of antibodies to IFNγ or control) and incubated at 37 °C for 1, 1.5–2, 2.5–3, or 3.5–4 h. Fluidised lactose was chosen since it is an excipient in the manufacture of drugs based on diluted antibodies to IFNγ. After incubation, spectra were recorded within a wavenumber range of 10 to 110 cm−1 with a resolution of 4 cm−1. Lactose saturated with dilutions of antibodies to IFNγ (incubated for more than 2.5 h) changed the structural properties of an IFNγ aqueous solution without direct contact compared to the control. Terahertz spectra revealed stronger intermolecular hydrogen bonds and an increase in the relaxation time of free and weakly bound water molecules. The methodology developed on the basis of THz-TDS could potentially be applied to quality control of pharmaceuticals based on extremely diluted antibodies.

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Section: Introductionmentioning

confidence: 99%

Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution

Penkov

2021

Pharmaceutics

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“…ELISA-enzyme immunoassay detects the ability of UHDs to modify their target, which leads to a change in the interaction between the target antigen and monoclonal antibodies to this antigen. The resulting antigen-antibody complexes are detected by enzyme-labeled secondary antibodies [13].…”

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confidence: 99%

The Supramolecular Matrix Concept

Epstein

2023

Symmetry

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It has been established that dilutions of a variety of substances, when exposed to vibration in the process of their preparation, acquire not only new structural characteristics in the form of nano-associates but also new physical properties, regardless of the presence of the initial substance. One of the most important properties of these dilutions is the ability to modify the physico–chemical and biological activity of the initial substance as well as exert non-contact, “distant”, effects. Here, we propose a novel hypothesis that the basis of modifying activity is the transformation of target molecules to a more harmonious (symmetrical) state supported by a supramolecular matrix, a structural unit of a structured space.

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The level of natural autoantibodies to IFN-gamma in varicella infection treated with antiviral drug Anaferon for children: A pilot study

Don

Meide²,

Egorov

et al. 2020

Immunology Letters

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Advanced approach to activity evaluation for released-active forms of antibodies to interferon-gamma by enzyme-linked immunoassay

Cited by 7 publications

References 26 publications

Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution

Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution

The Supramolecular Matrix Concept

The level of natural autoantibodies to IFN-gamma in varicella infection treated with antiviral drug Anaferon for children: A pilot study

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