Advanced glycation end products and adipocytokines and oxidative stress in placental tissues of pregnant women with gestational diabetes mellitus

Abstract

Correlation between expression levels of advanced glycation end products (AGEs) and adipocytokines and oxidative stress index malondialdehyde (MDA) in placental tissues of pregnant women with gestational diabetes mellitus (GDM) was investigated. Seventy-two cases of GDM pregnant women who received routine prenatal examination and gave birth in the Department of Obstetrics, Binzhou City Center Hospital from March 2016 to May 2017 were collected as the observation group. Another 80 cases of normal pregnant women who gave birth at the same time were selected as the control group. Enzyme linked immunosorbent assay (ELISA) was used to detect the expression levels of AGEs, visfatin, APN and IL-6 in the lysate of placental tissues. MDA levels were measured by thiobarbituric acid method. Correlation of expression levels of AGEs, visfatin, APN, IL-6 and MDA were analyzed. The expression level of MDA in placental tissues of the observation group was significantly higher than that of the control group (t=16.44, P<0.001). The correlation of expression levels between AGEs, adipocytokines and MDA in placental tissues of the two groups was analyzed, and it was found that the expression levels of AGEs, visfatin and IL-6 in the two groups were positively correlated with MDA. There was a significantly negative correlation between APN and MDA in the two groups. The incidence of cesarean section, neonatal hypoglycemia, fetal distress and macrosomia in the observation group was significantly higher than that in the control group (P<0.05). There was no significant difference in the incidence of membrane rupture and premature birth between the two groups (P>0.05). In conclusion, the expression levels of AGEs, visfatin and IL-6 in placental tissues of GDM pregnant women are positively correlated with MDA. There is a significant negative correlation between APN and MDA, and they play an important role in the pathogenesis of GDM.

Changes of Serum Adiponectin and Glycated Albumin Levels in Gestational Diabetes Mellitus Patients and Their Relationship with Insulin Resistance

“…The adiponectin level in GDM patients was significantly lower than that in normal pregnant women ( 22 ). GA level in GDM patients was higher than that in normal pregnant women ( 23 ), consistent with the results of this study. It showed that the occurrence of GDM was related to adiponectin and GA, which had an effect on the pathogenesis of GDM.…”

The relationship between advanced glycation end products and gestational diabetes: A systematic review and meta-analysis

“…The systematic review and meta-analysis involved studies that reported the outcome measures at both first and second trimesters [ 32 ], first and third trimesters [ 36 ], second trimester [ 30 , 31 , 34 , 41 , 44 ], second and third trimesters [ 38 , 42 ], and third trimester of pregnancy [ 33 , 35 , 37 , 39 , 40 ] whereas two studies did not specify gestational period during sample collection [ 43 , 45 ]. Blood sample was collected from 13 studies [ 30 , 31 , 33 , 35 – 40 , 42 – 45 ], skin sample from two studies [ 32 , 34 ], and placenta sample from one study [ 41 ] ( Table 1 ). For analysis of specific AGEs and metabolic biomarkers, five and four studies were included to generate the pooled estimates of HOMA-IR [ 31 , 37 , 42 , 43 , 45 ], and HbA1c [ 31 , 33 , 37 , 43 ], respectively.…”

“…Regarding the geographical distribution, the review included seven studies from Europe [30][31][32][33][34][35][36], seven studies from Asia [37][38][39][40][41][42][43] and two studies from Latin America [44,45]. Thirteen of these studies employed case-control design [30,32,33,[35][36][37][38][39][40][41][42][43][44] whereas the rest three studies were cross-sectional in design [31,34,45]. Specifically, the meta-analysis of AGEs involved 774 cases and 834 controls.…”

O-GlcNAcylation in Hyperglycemic Pregnancies: Impact on Placental Function

“…The placenta of women with HIP is exposed to a high concentration of blood glucose at different degrees and windows of time. This may affect numerous cellular pathways, leading to accumulation of advanced glycation end-products (4,5) and induction of oxidative stress (6). The reported activation of the chronic hexosamine biosynthetic pathway (HBP) in placental tissue under similar conditions is also garnering attention (7).…”