Advanced In Vitro Three-Dimensional Skin Models of Atopic Dermatitis

Jang, Hye-Jeong; Lee, Jung Bok; Yoon, Jong Il

doi:10.1007/s13770-023-00532-1

Cited by 8 publications

(2 citation statements)

References 148 publications

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“…Nowadays, ex vivo cellular and tissue models are being used to evaluate the efficacy and safety of pharmaceutical and cosmetic research, providing reliable and speedy results in the laboratory [66]. Three-dimensional (3D) skin models by cell culture were developed for AD skin [1,67,68]. Further studies with human artificial skin or bioengineered artificial skin substitutes such as 3D cell culture reconstructed human epithelium are suggested to be conducted to study toxicity, skin penetration, corrosive properties, or skin irritation before conducting an in vivo study with animal models or in a human trial [66].…”

Section: Discussionmentioning

confidence: 99%

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Liana,

Eurtivong,

Phanumartwiwath

2024

Antioxidants

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Human 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) are potential targets for suppressing pruritic skin inflammation in atopic dermatitis (AD). In addition, Staphylococcus aureus colonization and oxidative stress worsen AD skin conditions. We aimed to investigate anti-inflammatory activity, using 5-LOX and COX-2 inhibitions, and the anti-staphylococcal, and antioxidant potentials of several medicinal plants bio-prospected from traditional medicine related to AD pathogenesis. Essential oils and hexane fractions were prepared and analyzed using gas chromatography–mass spectrometry. Boesenbergia rotunda hexane extract displayed anti-Staphylococcus aureus (MIC = 10 µg/mL) and antioxidant activities (IC50 = 557.97 and 2651.67 µg/mL against DPPH and NO radicals, respectively). A major flavonoid, pinostrobin, was further nonchromatographically isolated. Pinostrobin was shown to be a potent 5-LOX inhibitor (IC50 = 0.499 µM) compared to nordihydroguaiaretic acid (NDGA; IC50 = 5.020 µM) and betamethasone dipropionate (BD; IC50 = 2.077 µM) as the first-line of AD treatment. Additionally, pinostrobin inhibited COX-2 (IC50 = 285.67 µM), which was as effective as diclofenac sodium (IC50 = 290.35 µM) and BD (IC50 = 240.09 µM). This kinetic study and molecular modeling showed the mixed-type inhibition of NDGA and pinostrobin against 5-LOX. This study suggests that B. rotunda and its bioactive pinostrobin have promising properties for AD therapy.

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Section: Discussionmentioning

confidence: 99%

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Liana,

Eurtivong,

Phanumartwiwath

2024

Antioxidants

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“…The skin comprises three layers: the epidermis, dermis, and subcutis. It is the largest organ of the body, covering the surface of our body and protecting it from the environment [ 36 , 213 ]. However, the human body often experiences skin trauma owing to external forces.…”

Section: Tissue Engineering Applicationsmentioning

confidence: 99%

Developing hydrogels for gene therapy and tissue engineering

Su,

Lin,

Huang

et al. 2024

J Nanobiotechnol

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Hydrogels are a class of highly absorbent and easily modified polymer materials suitable for use as slow-release carriers for drugs. Gene therapy is highly specific and can overcome the limitations of traditional tissue engineering techniques and has significant advantages in tissue repair. However, therapeutic genes are often affected by cellular barriers and enzyme sensitivity, and carrier loading of therapeutic genes is essential. Therapeutic gene hydrogels can well overcome these difficulties. Moreover, gene-therapeutic hydrogels have made considerable progress. This review summarizes the recent research on carrier gene hydrogels for the treatment of tissue damage through a summary of the most current research frontiers. We initially introduce the classification of hydrogels and their cross-linking methods, followed by a detailed overview of the types and modifications of therapeutic genes, a detailed discussion on the loading of therapeutic genes in hydrogels and their characterization features, a summary of the design of hydrogels for therapeutic gene release, and an overview of their applications in tissue engineering. Finally, we provide comments and look forward to the shortcomings and future directions of hydrogels for gene therapy. We hope that this article will provide researchers in related fields with more comprehensive and systematic strategies for tissue engineering repair and further promote the development of the field of hydrogels for gene therapy. Graphical abstract

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Advanced 3D Cell Culture Platform for Tissue Engineering

Yoon

2023

Tissue Eng Regen Med

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Advanced In Vitro Three-Dimensional Skin Models of Atopic Dermatitis

Cited by 8 publications

References 148 publications

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Boesenbergia rotunda and Its Pinostrobin for Atopic Dermatitis: Dual 5-Lipoxygenase and Cyclooxygenase-2 Inhibitor and Its Mechanistic Study through Steady-State Kinetics and Molecular Modeling

Developing hydrogels for gene therapy and tissue engineering

Advanced 3D Cell Culture Platform for Tissue Engineering

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