Advanced silk material spun by a transgenic silkworm promotes cell proliferation for biomedical application

Wang, Feng; Xu, Hanfu; Wang, Yuancheng; Wang, Riyuan; Lin, Yuan; 丁焕,; Song, Chunnuan; Ma, Sanyuan; Peng, Zhixin; Peng, Zhangchuan; Zhao, Ping; Xia, Qingyou

doi:10.1016/j.actbio.2014.06.031

Cited by 45 publications

(33 citation statements)

References 48 publications

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“…But, the baculovirus-infected silkworm is a binary and transient recombinant protein production system. Thus, various investigators began to genetically transform silkworms with DNA constructs encoding the protein of interest under the transcriptional control of either whole body (Tamura et al, 2000) or silk gland-specific promoters (Adachi et al, 2006; Hino et al, 2006; Iizuka et al, 2009; Iizuka et al, 2013; Kojima et al, 2007; Kurihara et al, 2007; Kuwana et al, 2014; Long et al, 2015; Ogawa et al, 2007; Royer et al, 2005; Seong et al, 2011; Teule et al, 2012; Tomita et al, 2007; Tomita et al, 2003; Wang et al, 2014; Wen et al, 2010; Xu, 2014; Yanagisawa et al, 2007; reviewed in Tomita, 2011). This new, single component approach provided a stable, rather than transient source of recombinant proteins.…”

Section: Introductionmentioning

confidence: 99%

Targeted glycoengineering extends the protein N-glycosylation pathway in the silkworm silk gland

Mabashi-Asazuma

Sohn

Kim

et al. 2015

Insect Biochemistry and Molecular Biology

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The silkworm silk glands are powerful secretory organs that can produce and secrete proteins at high levels. As such, it has been suggested that the biosynthetic and secretory power of the silk gland can be harnessed to produce and secrete recombinant proteins in tight or loose association with silk fibers. However, the utility of the silkworm platform is constrained by the fact that it has a relatively primitive protein N-glycosylation pathway, which produces relatively simple insect-type, rather than mammalian-type N-glycans. In this study, we demonstrate for the first time that the silk gland protein N-glycosylation pathway can be glycoengineered. We accomplished this by using a dual piggyBac vector encoding two distinct mammalian glycosyltransferases under the transcriptional control of a posterior silk gland (PSG)-specific promoter. Both mammalian transgenes were expressed and each mammalian N-glycan processing activity was induced in transformed silkworm PSGs. In addition, the transgenic animals produced endogenous glycoproteins containing significant proportions of mammalian-type, terminally galactosylated N-glycans, while the parental animals produced none. This demonstration of the ability to glycoengineer the silkworm extends its potential utility as a recombinant protein production platform.

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Section: Introductionmentioning

confidence: 99%

Targeted glycoengineering extends the protein N-glycosylation pathway in the silkworm silk gland

Mabashi-Asazuma

Sohn

Kim

et al. 2015

Insect Biochemistry and Molecular Biology

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“…While 39K exhibited relatively weak activity compared with a strong constitutive promoter. As is their nature, enhancers can greatly improve promoter activity, and many baculovirus enhancers have been well studied, including hrs and polh-up (Acharya and Gopinathan, 2001;Guarino and Dong, 1991;Lo et al, 2002;Lu et al, 1997;Rodems and Friesen, 1993;Wang et al, 2014). In order to further increase the 39K-inducible promoter activity, we analyzed and compared different enhancers in affecting the activity of 39K promoter.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies determined that hrs and polh-up have enhancer effects on various promoters of AcMNPV (Acharya and Gopinathan, 2001;Lo et al, 2002;Lu et al, 1997;Majima et al, 1993;Rodems and Friesen, 1993;Wang et al, 2014). To improve the BmNPV 39K promoter activity, we tested three enhancer sequences (hr3, hr5 and polh-up) for their enhancing effect on 39K promoter activity of BmNPV.…”

Section: Construction Of An Artificially Enhanced 39k Promotermentioning

confidence: 98%

Screening and optimization of an efficient Bombyx mori nucleopolyhedrovirus inducible promoter

Cao

Kuang

et al. 2016

Journal of Biotechnology

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“…In addition, more giant cells surrounding silk mesh were denoted compared to other commercial meshes (Ultrapro from Ethicon or Surgisis) but gradually vanished, along with the disappearance of the biomaterial, to create a collagenous scar in the wounded area . As natural silk is rather heterogeneous regarding mechanical and biological behaviors depending on the studied species (i.e., there are 44,540 known spider species), the production of engineered‐silks using transgenic organisms (bacteria or worms with enhanced performance offers remarkable advances and warrants more interest for the future manufacturing of hernia meshes.…”

Section: Biologically Derived Matricesmentioning

confidence: 99%

Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio‐Functionality to Meshes

Guillaume

Teuschl

Gruber-Blum

et al. 2015

Adv Healthcare Materials

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Abdominal wall hernia is a recurrent issue world-wide and requires the implantation of over 1 million meshes per year. Because permanent meshes such as polypropylene and polyester are not free of complications after implantation, many mesh modifications and new functionalities have been investigated over the last decade. Indeed, mesh optimization is the focus of intense development and the biomaterials utilized are now envisioned as being bioactive substrates that trigger various physiological processes in order to prevent complications and to promote tissue integration. In this context, it is of paramount interest to review the most relevant bio-functionalities being brought to new meshes and to open new avenues for the innovative development of the next generation of meshes with enhanced properties for functional abdominal wall hernia repair.

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Advanced silk material spun by a transgenic silkworm promotes cell proliferation for biomedical application

Cited by 45 publications

References 48 publications

Targeted glycoengineering extends the protein N-glycosylation pathway in the silkworm silk gland

Targeted glycoengineering extends the protein N-glycosylation pathway in the silkworm silk gland

Screening and optimization of an efficient Bombyx mori nucleopolyhedrovirus inducible promoter

Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio‐Functionality to Meshes

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