Advanced Studies on Virulence Genes of Salmonella and Shigella species Isolated from Milk and Dairy Products

Younis, Gamal; Elkenany, Rasha; Abd-Elmoati, Wesam S.

doi:10.9734/arrb/2018/44651

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…[13][14][15][16] Public health threat pathogenic bacteria of the Enterobacteriaceae family are mostly implicated in foodborne infections globally. 17,18 Salmonella spp., Shigella spp., Listeria spp., Escherichia coli, Enterobacter spp., Campylobacter spp. and Yersinia spp.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Antibiotic Resistance Profile of Public Health Threat Enteric Bacteria from Milk and Dairy Products Retail in Abakaliki, South-East, Nigeria

Chukwu,

Uzoh,

Yusuf

et al. 2023

J. Pure Appl. Microbiol.

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Milk and foods made from milk is manufactured into more stable dairy products of worldwide value, such as butter, cheese, ice cream, and yoghurt. Consumption of contaminated milk or dairy products by pathogens causes human gastrointestinal infection, which leads to diarrheal disease in human and hospitalization or death in severe cases especially among elderly and children. An assessment of milk and dairy products was designed to determine the microbiological quality of milk and dairy products consumed in Abakaliki, Nigeria. Culture techniques were used for isolation of enteric bacteria from retail dairy products and disk diffusion method were used to determine the Antibiotic Resistance profile of isolates. Bacteria pathogens isolated were characterized and identified using morphological and biochemical techniques. SPSS and Chi-square test were used for the analysis of the study, P-value of 0.02 indicates a significant difference between the bacteria pathogens counts. A total of 161 pathogenic bacteria were isolated from 100 dairy products. Salmonella spp heard (26.1%), Escherichia coli (44.1%) and Shigella spp. (29.8%). All identified isolates were found to be 100% susceptible to ciprofloxacin and gentamycin, with 66.7% for ofloxacin. Augmentin, ampicillin, chloramphenicol and spectinomycin was 100% resistant. Data obtained confirm that milk and dairy products retailed in Abakaliki pose a serious public health threat to consumers due to the presence of pathogenic bacteria. Standard and good storage conditions, as well as environmental and personnel hygiene should be practiced to prevent contamination of milk and dairy products for the safety of consumers.

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Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Antibiotic Resistance Profile of Public Health Threat Enteric Bacteria from Milk and Dairy Products Retail in Abakaliki, South-East, Nigeria

Chukwu,

Uzoh,

Yusuf

et al. 2023

J. Pure Appl. Microbiol.

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“…Raw milk and its products may become contaminated either directly or indirectly from different sources [ 2 ]. Pathogens in milk are a leading cause of foodborne infections, which is a collective public health issue for investigators and consumers [ 3 ]. Salmonellosis remains the major human foodborne illness transmitted through the consumption of contaminated milk and its products.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and antibiogram of multidrug-resistant Salmonella enterica isolated from dairy products in Libya

et al. 2022

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Background and Aim: Foodborne illnesses are a serious challenge to human health and the economic sector. For example, salmonellosis remains a burden in developed and developing nations. Rapid and reliable molecular methods to identify Salmonella strains are essential for minimizing human infection. This study aimed to identify Salmonella spp. in raw milk and dairy products using conventional and molecular techniques and to test the antibiotic susceptibility of the isolated strains. Materials and Methods: One hundred and thirty-one milk and dairy product samples were randomly collected from different localities in Libya. Samples were examined for the presence of Salmonella by conventional culture techniques, including cultivation in Rappaport-Vassiliadis broth and streaking on xylose lysine deoxycholate agar. Identification also used polymerase chain reaction and partial sequencing of 16S rDNA. Twenty-four antibiotics were used for the examination of antimicrobial resistance of Salmonella spp. isolates with the agar disk diffusion method (Kirby–Bauer technique). Multi-antibiotic resistance index and antibiotic resistance index (ARI)for Salmonella enterica isolates were calculated. Results: Twenty-one of 131 samples (16%) were positive for Salmonella spp. recovered from 9 (16%), 2 (11%), 4 (22.2%), and 6 (46%) samples of raw cow milk, fermented raw milk, and fresh locally made soft cheeses, Maasora and Ricotta), respectively. Samples of ice cream, milk powder, and infant formula showed no Salmonella spp. contamination. Only 9 of 21 (42.8%) isolates were confirmed as S. enterica by partial sequence 16S rDNA analysis. All isolates were resistant to amoxycillin, bacitracin, penicillin G, lincomycin, vancomycin, clindamycin, and cloxacillin with an ARI of 0.042. In contrast, all tested strains were sensitive to levofloxacin, doxycycline, and ciprofloxacin. In addition, all of the tested isolates (100%) were resistant to more than one antibiotic. Conclusion: This study demonstrated the applicability of molecular techniques, compared with conventional methods, as preferable for the identification of Salmonella in milk and dairy products and thus reduction of milk-borne transmission to the consumers. From the view of public health, isolation and identification of Salmonella multidrug-resistant strains from raw cow's milk and locally prepared dairy products sold in the Libyan markets indicate the need to improve the handling and processing of milk and dairy products to minimize the prevalence of Salmonella, one of the most important foodborne microorganisms that cause food poisoning.

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Advanced Studies on Virulence Genes of Salmonella and Shigella species Isolated from Milk and Dairy Products

Cited by 2 publications

References 36 publications

Isolation, Identification and Antibiotic Resistance Profile of Public Health Threat Enteric Bacteria from Milk and Dairy Products Retail in Abakaliki, South-East, Nigeria

Isolation, Identification and Antibiotic Resistance Profile of Public Health Threat Enteric Bacteria from Milk and Dairy Products Retail in Abakaliki, South-East, Nigeria

Occurrence and antibiogram of multidrug-resistant Salmonella enterica isolated from dairy products in Libya

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