Advanced Technologies in On-Site Detection of Genetically Modified Products

Li, Jiale; Li, Jie; Lin, Shenghao; Zhu, Longjiao; Li, Xiangyang; Xu, Wentao

doi:10.3390/agriculture12060888

Agriculture

2022

DOI: 10.3390/agriculture12060888

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Advanced Technologies in On-Site Detection of Genetically Modified Products

Jiale Li

Jie Li

Shenghao Lin

et al.

Abstract: Transgenic technology is significantly impacting life today. However, with the advancement of genetically modified technologies and the success of genetically modified product commercialization, new challenges have arisen for associated detecting technologies. The need for fast, precise, and portable systems for the on-site detection of genetically modified products has increased dramatically in recent years. This perspective examined the currently available technological support for portable immune biosensing… Show more

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Cited by 2 publications

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Event-specific loop-mediated isothermal amplification for living modified cotton MON88701, MON531, MON15985, MON88913, and COT102

Choi,

Yoon,

Lee

et al. 2024

Biotechnol Bioproc E

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Gossypium hirsutum L., commonly known as upland cotton, is cultivated globally for natural fiber, feed, and seed oil. To enhance agricultural productivity and quality, living modified (LM) cotton has been developed and utilized since the late 1990s. Due to environmental concerns, such as biodiversity risks associated with living modified organisms (LMOs), the Korean government, including the Ministry of Environment, has been conducting LMO natural environment monitoring and post-management projects. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay to detect five specific LM cotton events (MON88701, MON531, MON15985, MON88913, and COT102), which were the most abundant volunteers observed from 2019 to 2021 in South Korea. The event-specific LAMP assays for the five LM cotton events were established with a 40-min reaction time using LAMP reaction buffer, Bst DNA polymerase, and event-specific primers. The limit of detection ranged from 0.01 to 1 ng/μL. The assays' specificity and sensitivity were validated through colorimetric changes, fluorescence intensity measurements, and conventional PCR. To demonstrate the practical application of the LAMP assays, we tested 22 LM cotton volunteers collected from the natural environment in 2021. Each LAMP assay event-specifically amplified the respective LM cotton volunteers. These results indicate that the developed LAMP assays are effective tools for the efficient management and detection of LM cotton in field surveys, supporting regulatory compliance and environmental monitoring efforts.

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Event-specific loop-mediated isothermal amplification for living modified cotton MON88701, MON531, MON15985, MON88913, and COT102

Choi,

Yoon,

Lee

et al. 2024

Biotechnol Bioproc E

View full text Add to dashboard Cite

show abstract

Applications of microfluidics in biosensing

Sekhwama,

Mpofu,

Sivarasu

et al. 2024

Discov Appl Sci

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Microfluidic devices have become a vastly popular technology, particularly because of the advantages they offer over their traditional counterparts. They have such a wide range of uses and can make complex tasks quite efficient. One area of research or work that has benefited greatly from the use of microfluidics is biosensing, where microfluidic chips are integrated into biosensor setups. There are growing numbers of applications of microfluidics in this area as researchers look for efficient ways to tackle disease diagnostics and drug discovery, which are critical in this era of recurring pandemics. In this work, the authors review the integration of microfluidic chips with biosensors, as well as microfluidic applications in biosensing, food security, molecular biology, cell diagnostics, and disease diagnostics, and look at some of the most recent research work in these areas. The work covers a wide range of applications including cellular diagnostics, life science research, agro-food processing, immunological diagnostics, molecular diagnostics, and veterinarian diagnostics. Microfluidics is a field which combines fundamental laws of physics and chemistry to solve miniaturization problems involving fluids at the nanoscale and microscale, and as such, the authors also examine some fundamental mathematical concepts in microfluidics and their applications to biosensing. Microfluidics has relatively new technologies with great potential in terms of applications.

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Advanced Technologies in On-Site Detection of Genetically Modified Products

Cited by 2 publications

References 39 publications

Event-specific loop-mediated isothermal amplification for living modified cotton MON88701, MON531, MON15985, MON88913, and COT102

Event-specific loop-mediated isothermal amplification for living modified cotton MON88701, MON531, MON15985, MON88913, and COT102

Applications of microfluidics in biosensing

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