Advances in Analytical Technologies for Extracellular Vesicles

He, Yan; Li, Yutao; Cheng, Shi‐Bo; Zeng, Yong

doi:10.1021/acs.analchem.1c00693

Cited by 71 publications

(75 citation statements)

References 433 publications

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“…Li's group reported a COFsbased aptasensor for the analysis of CRC exosomes. 193 As shown in Figure 11D, spherical COFs with high porosity were utilized to load para-sulfocalix [4]arene hydrate (pSC 4 )-modified AuNPs and a large amount of HRP molecules to form HRP-pSC 4 -AuNPs@COFs. pSC 4 could specifically bind with various amino acid residues on the exosome surface.…”

Section: Sandwich-like Methodsmentioning

confidence: 99%

“…For the convenience of the reader, this review used a relatively broad term of "exosomes" to refer to a heterogeneous mixture of sEVs less than 200 nm in size because it is increasingly utilized in bioassays. 4 Compared to circulating tumor cells and circulating DNA, exosomes exist in body fluids (such as serum, urine and ascites) with higher abundance and stability. 5 Emerging evidences suggest that most types of cancer secret large numbers of exosomes that carry abundant molecular information stemming from parent tumor cells, including nucleic acids, proteins, bioactive lipids and metabolites.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, numerous novel biosensors with high sensitivity and efficiency have been established for the determination of exosomes by specific recognition, including colorimetry, fluorescence, surface plasmon resonance (SPR), surface enhanced Raman scattering (SERS) spectroscopy, mass spectrometry and electrochemical, electrochemiluminescent (ECL) and photoelectrochemical (PEC) assays. 4,[52][53][54][55][56][57][58][59] Moreover, many works based on microfluidic devices implemented with optical or electrochemical techniques have been reported for the comprehensive assays of exosomes. [60][61][62][63][64] With the growing development of nanotechnology, myriad nanomaterials and nanostructures have made a great impact on biosensing.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

Ma¹,

Hao²,

Liu³

2021

IJN

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Section: Sandwich-like Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

Ma¹,

Hao²,

Liu³

2021

IJN

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“…So far, apart from traditional methods including nanoparticle tracking analysis, western blot, ow cytometry, and enzyme-linked immunosorbent assay [11], various biosensing platforms have been developed for exosomes analysis by targeting their surface proteins using the corresponding antibodies or aptamers [12][13][14][15][16][17]. Among them, surface plasma resonance (SPR) biosensor is receiving extensive attention because it is a rapid, real-time, and label-free diagnostic device [18,19].…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon Resonance Biosensor for Sensitive Detection of HER2-positive Exosomes Based on Reformative Tyramine Signal Amplification Activated by Molecular Aptamer Beacon Conversion

Chen

et al. 2021

Preprint

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HER2-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G4 DNA that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated AuNPs (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of nature enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application.

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“…It has been reported that the expression of exosomal membrane proteins such as CD8, CD63, EpCAM, PCAT-1 and PSA is much higher on cancerous exosomes than on noncancerous exosomes 5,6 . Therefore, exosomes have been proposed as biomarkers for the early noninvasive diagnosis of cancer 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Fast and specific enrichment of cancer-related exosomes by DNA-nanoweight-assisted centrifugation

yuxyan

Shi³

et al. 2021

Preprint

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Exosomes are nanoscale membrane vesicles actively released by cells and play an important role in the diagnosis of cancer-related diseases. However, it is challenging to efficiently enrich exosomes from extracellular fluids. In this work, we used DNA-tetrahedron as a nanoweight during centrifugation to precisely enrich tumor exosomes from a complex biological environment. Two different DNA tetrahedral nanostructures (DTAs), each carrying a specific aptamer for exosome biomarker recognition, were incubated with clinical samples simultaneously. One DTA triggered the cross-linking of multiple target exosomes, and therefore enabled low-speed and fast centrifugation for enrichment. The other DTA further narrowed down the target exosome subtype and initiated a hybridization chain reaction (HCR) for sensitive signal amplification. The method enabled the detection of 180 MCF-7-derived exosomes per microliter and 560 HepG2-derived exosomes per microliter, with 1000-fold higher sensitivity than conventional ELISA. This easy-to-operate method can enrich exosomes with excellent specificity and therefore will be appealing in biomedical research and clinical diagnosis.

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Advances in Analytical Technologies for Extracellular Vesicles

Cited by 71 publications

References 433 publications

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

Surface Plasmon Resonance Biosensor for Sensitive Detection of HER2-positive Exosomes Based on Reformative Tyramine Signal Amplification Activated by Molecular Aptamer Beacon Conversion

Fast and specific enrichment of cancer-related exosomes by DNA-nanoweight-assisted centrifugation

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