Advances in Aptamer-Based Biomarker Discovery

Huang, Jie; Chen, Xinxin; Fu, Xuekun; Li, Zheng; Huang, Yühong; Liang, Chao

doi:10.3389/fcell.2021.659760

Cited by 54 publications

(49 citation statements)

References 87 publications

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“…Table 2 summarizes important recent studies on biomolecule-functionalized magnetic iron oxide nanoparticles for biomedical applications covered in this section. In recent years, aptamer-enabled technologies have attracted significant attention in the research community as a sensitive, reliable, and convenient method for biomarker detection in various critical human diseases [48]. Combining aptamers and IONPs offers a new set of approaches to develop more effective medical technologies for both diagnostic and therapeutic applications.…”

Section: Integration Of Biomolecules and Magnetic Iron Oxide Nanopart...mentioning

confidence: 99%

Multifunctional Iron Oxide Magnetic Nanoparticles for Biomedical Applications: A Review

et al. 2022

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Due to their good magnetic properties, excellent biocompatibility, and low price, magnetic iron oxide nanoparticles (IONPs) are the most commonly used magnetic nanomaterials and have been extensively explored in biomedical applications. Although magnetic IONPs can be used for a variety of applications in biomedicine, most practical applications require IONP-based platforms that can perform several tasks in parallel. Thus, appropriate engineering and integration of magnetic IONPs with different classes of organic and inorganic materials can produce multifunctional nanoplatforms that can perform several functions simultaneously, allowing their application in a broad spectrum of biomedical fields. This review article summarizes the fabrication of current composite nanoplatforms based on integration of magnetic IONPs with organic dyes, biomolecules (e.g., lipids, DNAs, aptamers, and antibodies), quantum dots, noble metal NPs, and stimuli-responsive polymers. We also highlight the recent technological advances achieved from such integrated multifunctional platforms and their potential use in biomedical applications, including dual-mode imaging for biomolecule detection, targeted drug delivery, photodynamic therapy, chemotherapy, and magnetic hyperthermia therapy.

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Section: Integration Of Biomolecules and Magnetic Iron Oxide Nanopart...mentioning

confidence: 99%

Multifunctional Iron Oxide Magnetic Nanoparticles for Biomedical Applications: A Review

et al. 2022

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“…1b). 33,34 However, it is worth noting that the presence of other untargeted proteins on the cell surface would result in the off-target selection. Thereby a counter selection using negative cells without expression of target proteins is introduced in the cell-based SELEX approach to eliminate off-target sequences.…”

Section: Cell-based Selex Approachmentioning

confidence: 99%

Aptamers: an emerging navigation tool of therapeutic agents for targeted cancer therapy

et al. 2022

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“…This suggests that NGS will be routinely used in the microbiology lab. Finally, there is an increase in the rapid availability of high-affinity specific binding reagents other than functional adhesins, such as (monoclonal) antibodies, adhirons and aptamers [ 26 , 27 ]. These have the advantage that they share a basic molecular structure, rendering them suitable for “plug and play” diagnostic applications using the same platforms.…”

Section: Introductionmentioning

confidence: 99%

Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens

Belkum¹,

Almeida²,

Bardiaux

et al. 2021

Diagnostics

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Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in developing or improving IVD. In this regard, an overlooked feature is the capacity of pathogens to adhere specifically to host cells and tissues. The molecular entities relevant for pathogen–surface interaction are the so-called adhesins. Adhesins vary from protein compounds to (poly-)saccharides or lipid structures that interact with eukaryotic host cell matrix molecules and receptors. Such interactions co-define the specificity and sensitivity of a diagnostic test. Currently, adhesin-receptor binding is typically used in the pre-analytical phase of IVD tests, focusing on pathogen enrichment. Further exploration of adhesin–ligand interaction, supported by present high-throughput “omics” technologies, might stimulate a new generation of broadly applicable pathogen detection and characterization tools. This review describes recent results of novel structure-defining technologies allowing for detailed molecular analysis of adhesins, their receptors and complexes. Since the host ligands evolve slowly, the corresponding adhesin interaction is under selective pressure to maintain a constant receptor binding domain. IVD should exploit such conserved binding sites and, in particular, use the human ligand to enrich the pathogen. We provide an inventory of methods based on adhesion factors and pathogen attachment mechanisms, which can also be of relevance to currently emerging pathogens, including SARS-CoV-2, the causative agent of COVID-19.

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Advances in Aptamer-Based Biomarker Discovery

Cited by 54 publications

References 87 publications

Multifunctional Iron Oxide Magnetic Nanoparticles for Biomedical Applications: A Review

Multifunctional Iron Oxide Magnetic Nanoparticles for Biomedical Applications: A Review

Aptamers: an emerging navigation tool of therapeutic agents for targeted cancer therapy

Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens

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