Advances in characterization and epidemiology of strawberry viruses and phytoplasmas in Argentina

Conci, V. C.; Luciani, C. A.; Merino, Mariano Lisandro; Celli, Marcos Giovani; Perotto, María Cecilia; Torrico, A. K.; Pozzi, Elizabeth Alicia; Strumia, G.; Dughetti, A. C.; Asinari, Florencia; Conci, Luis Rogelio; Fernández, Franco D.; Salazar, Sergio Miguel; Meneguzzi, Natalia; Kirschbaum, Daniel Santiago

doi:10.17660/actahortic.2017.1156.118

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…Viruses in strawberry plants are found at low concentrations and in mixed infections, and commonly induce non-specific plant symptoms [3,4]. In particular, multiple viral infections in plants can lead to yield loss and plant decline [2,3,5,6,7,8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Fráňová

Přibylová

Koloniuk

2019

Viruses

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Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects F. ananassa and F. vesca plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single F. ananassa host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via Aphis fabae and A. ruborum, further mechanically transmitted to Nicotiana occidentalis 37B and sub-inoculated to N. benthamiana, N. benthamiana DCL2/4i, N. occidentalis 37B, and Physalis floridana plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed F. vesca clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus Cytorhabdovirus. Acyrthosiphon malvae, A. fabae, and A. ruborum were shown to transmit StrV-1 under experimental conditions.

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Section: Introductionmentioning

confidence: 99%

Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Fráňová

Přibylová

Koloniuk

2019

Viruses

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“…Из наиболее распространённых и вредоносных преобладают вирусы мозаики резухи, кольцевой пятнистости малины, латентной кольцевой пятнистости земляники, черной кольцевой пятнистости томата [8,9]. В насаждениях земляники садовой вирусы распространяются с зараженным посадочным материалом, нематодами семейства Longidoridae (Xiphinema diversicaudatum -вектор ArMV и SLRSV, Longidoruselongates -вектор RpRSV иTBRV, тлями (CMV) [8,9,10,11]. Вирусы на землянике приводят к снижению урожая до 30 % и ухудшению качественных показателей [10,12].…”

Section: For the Successful Functioning Of A Breeding And Nursery Center Of Scientific And Practical Work With Fruit And Small Fruit Cropunclassified

Features of the strawberry field repository formation

Upadyshev

Tumaeva

Борисова

et al. 2021

Аграрная наука Евро-Северо-Востока

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For the successful functioning of a breeding and nursery center of scientific and practical work with fruit and small fruit crops, an important task is to create repositories, including thosein the field. A field repository is a plant gene bank based in accordance with international standards on planting material that is free from dangerous pathogens, including viruses, representing tested for productivity typical plants.For the purpose of a comparative study of promising varieties, hybrids and clones-candidates for original plants, a field repository and mother plantation of strawberries clones and varieties have been created on the territory of the Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery.As a result of research in 2015-2020, 386 high-yielding strawberry plants were selected and tested for the main harmful viruses using diagnostic kits from “Loewe” firm (Germany). The prevalence of harmful Arabis mosaic virus (ArMV), Raspberry ringspot virus (RpRSV), Tomato black ring virus (TBRV), Strawberry latent ringspot virus (SLRSV), Cucumber mosaic virus (CMV) in strawberry plantations depended on the area cultivation, varietal composition of plantings and ranged from 31 to 69 %. The prevalence of viruses RpRSV (up to 36 %), TBRV (up to 31 %) and CMV (up to 22 %) was established. The high efficiency of dry-air thermotherapy for the recovery of strawberries with the number of virus-free intact plants of 56 % has been shown.A genebank of "candidates for original plants" has been formed from 234 strawberry plants of 39 varieties and hybrids, which, after confirming their status by PCR, will be transferred to the category of "original plants".

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“…Viruses in strawberry plants are found at low concentrations and in mixed infections and commonly induce non-specific plant symptoms [1,2]. In particular, multiple viral infections in plants can lead to plant decline, yield losses and plant death [1,[3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Molecular and biological characterization of a new strawberry Cytorhabdovirus

Fráňová

Přibylová

Koloniuk

2019

Preprint

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Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects F. ananassa and F. vesca plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single F. ananassa host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via Aphis fabae and A. ruborum, further mechanically transmitted to Nicotiana occidentalis 37B and sub-transferred to N. benthamiana, N. benthamiana DCL2/4i, N. occidentalis 37B and Physalis floridana plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed F. vesca clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus Cytorhabdovirus. Acyrthosiphon malvae, A. fabae and A. ruborum were shown to transmit StrV-1 under experimental conditions.

show abstract

Advances in characterization and epidemiology of strawberry viruses and phytoplasmas in Argentina

Cited by 7 publications

References 28 publications

Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Features of the strawberry field repository formation

Molecular and biological characterization of a new strawberry Cytorhabdovirus

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