Advances in Colorimetric Assay Based on AuNPs Modified by Proteins and Nucleic Acid Aptamers

Melikishvili, Sopio; Piovarci, Ivan; Hianik, Tibor

doi:10.3390/chemosensors9100281

Cited by 21 publications

(12 citation statements)

References 159 publications

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“…Any change in the physical-chemical parameters related to the analyte binding can be exploited toward its quantification. Among all the protocols described (e.g., mass sensitive [ 142 , 143 ], colorimetric [ 144 ], fluorescence biosensors [ 145 ], etc. ), electrochemical methods are most frequently applied for this purpose.…”

Section: Principles Of Biosensors and Electrochemical Methods Of Anti...mentioning

confidence: 99%

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Evtugyn

Porfireva

Tsekenis

et al. 2022

Sensors

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Antibiotics are often used in human and veterinary medicine for the treatment of bacterial diseases. However, extensive use of antibiotics in agriculture can result in the contamination of common food staples such as milk. Consumption of contaminated products can cause serious illness and a rise in antibiotic resistance. Conventional methods of antibiotics detection such are microbiological assays chromatographic and mass spectroscopy methods are sensitive; however, they require qualified personnel, expensive instruments, and sample pretreatment. Biosensor technology can overcome these drawbacks. This review is focused on the recent achievements in the electrochemical biosensors based on nucleic acid aptamers for antibiotic detection. A brief explanation of conventional methods of antibiotic detection is also provided. The methods of the aptamer selection are explained, together with the approach used for the improvement of aptamer affinity by post-SELEX modification and computer modeling. The substantial focus of this review is on the explanation of the principles of the electrochemical detection of antibiotics by aptasensors and on recent achievements in the development of electrochemical aptasensors. The current trends and problems in practical applications of aptasensors are also discussed.

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Section: Principles Of Biosensors and Electrochemical Methods Of Anti...mentioning

confidence: 99%

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Evtugyn

Porfireva

Tsekenis

et al. 2022

Sensors

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“…36,37 Due to the merits of quick response, low cost, and no requirement of sophisticated instruments and professional operators, colorimetric sensors as a novel strategy have drawn tremendous attention. [38][39][40][41] Thus, simple colorimetric assays-based nanozymes offer the unflinching biocatalytic potential for portable and inexpensive daily applications.…”

Section: Zhongmei Chimentioning

confidence: 99%

Recent advances in colorimetric sensors based on nanozymes with peroxidase-like activity

Chi

Wang

2023

Analyst

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“…The aptamers sensitive to E. coli, which is a very common milk contaminant [11][12][13][14], have been used so far mostly for the development of electrochemical [10,15] and optical [16,17] aptasensors. There is, however, only a limited number of papers published so far on E. coli detection by acoustics methods.…”

Section: Introductionmentioning

confidence: 99%

Detection of E. coli Bacteria in Milk by an Acoustic Wave Aptasensor with an Anti-Fouling Coating

Spagnolo

Franier

Davoudian

et al. 2022

Sensors

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Milk is a significant foodstuff around the world, being produced and consumed in large quantities. The safe consumption of milk requires that the liquid has an acceptably low level of microbial contamination and has not been subjected to spoiling. Bacterial safety limits in milk vary by country but are typically in the thousands per mL of sample. To rapidly determine if samples contain an unsafe level of bacteria, an aptamer-based sensor specific to Escherichia coli bacteria was developed. The sensor is based on an ultra-high frequency electromagnetic piezoelectric acoustic sensor device (EMPAS), with the aptamer being covalently bound to the sensor surface by the anti-fouling linker, MEG-Cl. The sensor is capable of the selective measurement of E. coli in PBS and in cow’s milk samples down to limits of detection of 35 and 8 CFU/mL, respectively, which is well below the safe limits for commercial milk products. This sensing system shows great promise for the milk industry for the purpose of rapid verification of product safety.

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Advances in Colorimetric Assay Based on AuNPs Modified by Proteins and Nucleic Acid Aptamers

Abstract: [...]

Cited by 21 publications

References 159 publications

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Recent advances in colorimetric sensors based on nanozymes with peroxidase-like activity

Detection of E. coli Bacteria in Milk by an Acoustic Wave Aptasensor with an Anti-Fouling Coating

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