Advances in DNA-directed immobilization

Meyer, Rebecca; Giselbrecht, Stefan; Rapp, Bastian E.; Hirtz, Michael; Niemeyer, Christof M.

doi:10.1016/j.cbpa.2013.10.023

Cited by 95 publications

(100 citation statements)

References 76 publications

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“…Numerous current works regarding the machine-assisted production and analysis of small-molecule compounds, [70] DNAm olecules, [71] colloidal nanoparticles, [72] and technical platforms for handling and analysis of DNA nanostructures [73] and DNAs urfaces [23,74] indicate that the development of integrated systems involves lab-on-a-chip devices and robotics along with in-line analytics and data acquisition. Numerous current works regarding the machine-assisted production and analysis of small-molecule compounds, [70] DNAm olecules, [71] colloidal nanoparticles, [72] and technical platforms for handling and analysis of DNA nanostructures [73] and DNAs urfaces [23,74] indicate that the development of integrated systems involves lab-on-a-chip devices and robotics along with in-line analytics and data acquisition.…”

Section: Discussionmentioning

confidence: 99%

“…This allows for the convenient removal of IVTT components and makes it possible to investigate the interaction potential of several thousand proteins with other binding partners.W hen the DNAc hips are embedded in microfluidic lab-on-a-chip systems,g lobal reaction parameters can be tightly controlled to improve experimental reliability,t hroughput, and multiplexing capabilities with concomitant reduction of reagent consumption and processing time. [23,27] Them ethod is not limited to glass or polymer surfaces but can also be used for silicon, gold, and other materials.E xemplary applications include the plasmonic detection of small molecules, [28] electrochemical screening of enzymes, [29] or the investigation of electron transfer processes between proteins and metal surfaces. Invented in the early 1990s, [26] DDI takes advantage of surface-bound capture oligonucleotides that selectively hybridize with sets of proteins tagged with complementary oligonucleotides ( Figure 2B).…”

Section: Dna Chip-based Protein Analysismentioning

confidence: 99%

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DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Schneider

Niemeyer

2018

Angewandte Chemie

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The evolution of DNA microarray technology has led to sophisticated DNA chips that are being used as routine tools for fundamental and applied genome research such as genotyping and expression profiling. Owing to their capability for highly parallel, site‐directed immobilization of complementary nucleic acids through canonical Watson–Crick base‐pairing, however, DNA‐modified surfaces can also be used for the assembly of complex surface architectures comprised of non‐nucleic acid compounds, such as proteins or colloidal materials. Furthermore, implementation of functional DNA devices and structural DNA nanotechnology can unlock the full potential of DNA surfaces. Based on case studies from diagnostics, sensing, proteome research, cell adhesion, and cell signaling, we show how classical gene sensors have developed into modern integrated systems and platforms for various applications in life sciences and materials research.

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Section: Discussionmentioning

confidence: 99%

Section: Dna Chip-based Protein Analysismentioning

confidence: 99%

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Schneider

Niemeyer

2018

Angewandte Chemie

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“…[194] It also has possibility to simply convert an oligonucleotide array to an antibody array for high-throughput analysis. [198]…”

Section: Antibody Immobilization Chemistriesmentioning

confidence: 99%

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

Shen

Rusling

Dixit

2017

Methods

165

109

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Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field. With all these new approaches at our disposal, several new immobilization methods have been created to address the main challenges associated with immobilized antibodies. Few of these challenges that we have discussed in this review are mainly associated to the site-specific immobilization, appropriate orientation, and activity retention. We have discussed the effect of antibody immobilization approaches on the parameters on the performance of an immunoassay.

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“…The functionalized DNA can be stored for long period of time, and it is relatively simple to chemically modify or functionalize as needed. Such spacers enable a variety of applications including the recovery and reconfiguration of biosensor surfaces and fabrication of arrays in generation of biochips [19]. …”

Section: Dhfr Immobilizationmentioning

confidence: 99%

Determination of concentration and activity of immobilized enzymes

Singh

Morris

Tivanski

et al. 2015

Analytical Biochemistry

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Methods that directly measure the concentration of surface-immobilized biomolecules are scarce. More commonly the concentration of the soluble molecule is measured before and after immobilization, and the bound concentration is assessed by elimination, assuming that all bound molecules are active. An assay was developed for measuring the active-sites concentration, activity, and thereby the catalytic turnover rate (kcat) of an immobilized dihydrofolate reductase as a model system. The new method yielded a similar first order rate constant, kcat, to that of the same enzyme in solution. The findings indicate that the activity of the immobilized enzyme, when separated from the surface by the DNA spacers, has not been altered. Additionally, a new immobilization method is described that leads to solution-like activity of the enzyme on the surface. The approaches developed here for immobilization and for determining the concentration of an immobilized enzyme is general and can be extended to other enzymes, receptors, and antibodies.

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Advances in DNA-directed immobilization

Cited by 95 publications

References 76 publications

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

Determination of concentration and activity of immobilized enzymes

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