Advances in eggshell membrane separation and solubilization technologies

Abstract

Eggshell membranes (ESM) contain 90% protein, 3% lipids, 2% sugars, and small amounts of minerals such as calcium and magnesium. Of the 90% of proteins present, 472 proteins species have been identified. ESM provide the initial mineralization platform for eggshell formation, and can be used for to produce adsorbents, cosmetics, and medical products because of their special physical structure and chemical composition. The special physical structure of the eggshell membrane, with disulfide bonds between and within the protein molecules and the cross-linking of lysine-derived and heterochain chains between the eggshell membrane, makes the membrane very difficult to dissolve, with a maximum solubility rate of only 62%. Also, the insolubility of ESM limits its development and use also any related research. Based on the physical structure and chemical composition of the eggshell membrane, this paper reviews the latest research on eggshell membrane separation and membrane protein solubilization to provide a reference for promoting the separation, dissolution, and rational development and use of the avian eggshell membrane.

X‐Ray Visible Protein Scaffolds by Bulk Iodination

“…We also labeled egg‐shell membrane (ESM) (Figure 1H ), which contains a number of proteins alongside type I and type IV collagen. [ 60 ] Again, an increase of ≈200 HU was achieved with KI 3 /PBS (Figure 1H ), and labeling stability was preserved over 15 months (Figures S5 .and Video S3 , Supporting Information). We next demonstrated that a commercially available hydrogel of “Type 0” jellyfish‐derived collagen could also be labeled and imaged with an increased radiopacity of ≈280 HU (Figure 1I ).…”

Anti-inflammatory and regenerative effects of MKARE® Eggshell Membrane: an in vitro osteoarthritis model and placebo-controlled clinical study.

“…The ESM has demonstrated signi cant therapeutic potential in wound healing and tissue repair. Due to its multifaceted composition (Han et al, 2023), ESM emerges as a compelling candidate for addressing in ammatory processes linked to osteoarthritis (OA) and promoting the restoration of chondrocyte function (Kiers & Bult, 2021).…”