Advances in extraction technologies: isolation and purification of bioactive compounds from biological materials

Srivastava, N. K.; Singh, Arti; Kumari, Puja; Nishad, Jay Hind; Gautam, Veer Singh; Yadav, Monika; Bharti, Rajnish; Kumar, Dharmendra; Kharwar, Ravindra N.

doi:10.1016/b978-0-12-820655-3.00021-5

Cited by 34 publications

(25 citation statements)

References 58 publications

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“…In this process, the plant is grinded and mixed with the solvent and kept in a closed vessel for a minimum of 3 days. It is usually a repeated extraction in order to increase the extraction yield [41].…”

Section: Introductionmentioning

confidence: 99%

Reclaim and Valorization of Sea Buckthorn (Hippophae rhamnoides) By-Product: Antioxidant Activity and Chemical Characterization

Luntraru¹,

Apostol²,

Oprea

et al. 2022

Foods

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The by-product resulting from the production of the sea-buckthorn (Hippophae rhamnoides) juice may be a functional food ingredient, being a valuable source of bioactive compounds, such as polyphenols, flavonoids, minerals, and fatty acids. For checking this hypothesis, two extracts were obtained by two different methods using 50% ethyl alcohol solvent, namely through maceration–recirculation (E-SBM) and through ultrasound extraction (E-SBUS), followed by concentration. Next, sea-buckthorn waste (SB sample), extracts (E-SBM and E-SBUS samples) and the residues obtained from the extractions (R-SBM and R-SBUS samples) were characterized for the total polyphenols, flavonoid content, antioxidant capacity, mineral contents, and fatty acids profile. The results show that polyphenols and flavonoids were extracted better by the ultrasound process than the other methods. Additionally, the antioxidant activity of the E-SBUS sample was 91% higher (expressed in Trolox equivalents) and approximately 45% higher (expressed in Fe2+ equivalents) than that of the E-SBM sample. Regarding the extraction of minerals, it was found that both concentrated extracts had almost 25% of the RDI value of K and Mg, and also that the content of Zn, Mn, and Fe is significant. Additionally, it was found that the residues (R-SBM and R-SBUS) contain important quantities of Zn, Cu, Mn, Ca, and Fe. The general conclusion is that using the ultrasound extraction method, followed by a process of concentrating the extract, a superior recovery of sea-buckthorn by-product resulting from the juice extraction can be achieved.

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Section: Introductionmentioning

confidence: 99%

Reclaim and Valorization of Sea Buckthorn (Hippophae rhamnoides) By-Product: Antioxidant Activity and Chemical Characterization

Luntraru¹,

Apostol²,

Oprea

et al. 2022

Foods

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“…The powdered root bark of G. celebica (7.5 kg) was extracted with EtOAc (3 days × 10 L) through maceration, and the extract was concentrated in vacuo to yield 966.0 g of extract. The maceration process is a prevalent technique for the extraction of active compounds from plant materials [ 5 ]. Generally, the isolation of natural products needs a large amount of sample and maceration is suitable for this condition.…”

Section: Methodsmentioning

confidence: 99%

“…The antioxidant potential assay of the extracts and isolated compounds from G. celebica based on a single electron transfer reaction or hydrogen atom transfer reaction comprised the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferricreducing antioxidant power (FRAP). Table 3 shows the antioxidant activities of the extracts and compounds, with the highest antioxidant activity exhibited by the CH 2 Cl 2 extract for DPPH (IC 50 7.45 ± 0.04 µg/mL), followed by the n-hexane extract for ABTS assay (IC 50 5.96 ± 0.00 µg/mL), and the EtOAc extract by FRAP assay (66.36 ± 5.05 µM Fe 2+ /g). Furthermore, xanthochymol 3 exhibited stronger antioxidant activities by DPPH assay (2.33 ± 0.06 µg/mL or 3.87 ± 0.10 µM), ABTS assay (0.53 ± 0.01 µg/mL or 0.88 ± 0.02 µM), and FRAP assay (44.28 ± 0.79 µM Fe 2+ /g).…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…Furthermore, medicinal plants have been used worldwide as complementary or alternative medicines; therefore, studies on these medicinal plants, including biological and pharmacological evaluations, are important for drug discovery. Numerous medicinal compounds have been discovered from medicinal plants, for instance, the cancer drugs vincristine and vinblastine were isolated from Catharanthus roseus , paclitaxel was isolated from Taxus baccata , the malaria drug quinine was obtained from Cinchona ledgeriana , and artemisinin was isolated for the first time from Artemisia annua L. [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Biological Activity Evaluation and In Silico Studies of Polyprenylated Benzophenones from Garcinia celebica

et al. 2021

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This study aimed to isolate polyprenylated benzophenones from the rootbark of Garcinia celebica and assess their activities in vitro and in silico. The antioxidant activity was evaluated by the DPPH, ABTS, and FRAP methods. The cytotoxicity was evaluated against HeLa, MCF-7, A549, and B16 cancer cell lines. The antiplasmodial activity was performed against the chloroquine-sensitive Plasmodium falciparum strain 3D7. Molecular docking was analyzed on alpha-estrogen receptor (3ERT) and P. falciparum lactate dehydrogenase enzyme (1CET). The prediction of ADMET for the compounds was also studied. For the first time, (-)-cycloxanthochymol, isoxanthochymol, and xanthochymol were isolated from the root bark of Garcinia celebica. The antioxidant and cytotoxicity evaluation showed that all benzophenones exhibited antioxidant activity compared to gallic acid and quercetin as positive controls and also exhibited strong activity against HeLa, MCF-7, A549, and B16 cell lines compared to cisplatin as the positive control. The antiplasmodial evaluation showed that isoxanthochymol exhibited activity against the chloroquine-sensitive P. falciparum strain 3D7. In addition, the in silico molecular docking study supported in vitro activities. The ADMET analysis also indicated the isolated benzophenones are potential oral drug candidates.

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“…Bioactive compounds are molecules that are gaining popularity among scientists, primarily because of their many beneficial nutritional and medicinal properties. They are also increasingly used in a variety of industries (Srivastava et al ., 2021). Oregano is a traditional Mediterranean plant belonging to the mint family Lamiaceae with health properties due to its various bioactive compounds, mainly polyphenols (Kintzios, 2012).…”

Section: Introductionmentioning

confidence: 99%

Extraction of phenolic compounds from oregano using high voltage electrical discharges–sustainable perspective

Nutrizio

Jambrak

Rezić

et al. 2021

Int J of Food Sci Tech

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Summary The aim of the study was to observe the sustainability parameters of high voltage electrical discharge (HVED) as a novel technology for extraction of phenolic compounds from oregano. The life cycle assessment (LCA) of HVED was evaluated and compared with conventional extraction methods–infusion and maceration. The results showed that HVED extraction with nitrogen gave 2.19%–34.04% higher yield of phenolic compounds compared to infusion and 6.13%–90.95% higher yield compared to maceration, depending on the voltage and treatment time used for HVED. The environmental pillar of LCA, assessed by global warming potential, ranged from 1.130 to 4.635 kg CO2e for HVED samples treated with nitrogen and from 1.207 to 4.487 kg CO2e for samples treated with argon, which was mainly related to the duration of treatment. Other pillars of sustainability, namely society, economy, food safety and quality, were discussed. HVED was found to be the more sustainable technology (based on the results from LCA), with prospects for future scale‐up for the food industry.

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Advances in extraction technologies: isolation and purification of bioactive compounds from biological materials

Cited by 34 publications

References 58 publications

Reclaim and Valorization of Sea Buckthorn (Hippophae rhamnoides) By-Product: Antioxidant Activity and Chemical Characterization

Reclaim and Valorization of Sea Buckthorn (Hippophae rhamnoides) By-Product: Antioxidant Activity and Chemical Characterization

Biological Activity Evaluation and In Silico Studies of Polyprenylated Benzophenones from Garcinia celebica

Extraction of phenolic compounds from oregano using high voltage electrical discharges–sustainable perspective

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