Advances in isothermal nucleic acid amplification methods for hepatitis B virus detection

Li, Huilin; Song, Wenjun; Li, Hongying; Cui, Jiaqi; Xie, Yuchen; Wu, Bo; Chen, Rong

doi:10.1039/d3an00700f

Cited by 5 publications

(2 citation statements)

References 103 publications

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“…This study explores the advancements and applications of nucleic acid isothermal amplification-based platforms in the context of RNA virus detection. We delve into the diverse methodologies, including loop-mediated isothermal amplification (LAMP) [11,12], recombinase polymerase amplification (RPA) [13,14], recombinase-aided amplification (RAA) [15,16], nucleic acid sequence-based amplification (NASBA) [17,18], and helicasedependent amplification (HDA) [19,20], elucidating their principles, key components, and amplification mechanisms. This review focuses on the most common isothermal amplification methods, including LAMP, RPA, and RAA.…”

Section: Introductionmentioning

confidence: 99%

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Ngoc,

Lee

2024

Biosensors

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Ribonucleic acid (RNA) viruses are one of the major classes of pathogens that cause human diseases. The conventional method to detect RNA viruses is real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), but it has some limitations. It is expensive and time-consuming, with infrastructure and trained personnel requirements. Its high throughput requires sophisticated automation and large-scale infrastructure. Isothermal amplification methods have been explored as an alternative to address these challenges. These methods are rapid, user-friendly, low-cost, can be performed in less specialized settings, and are highly accurate for detecting RNA viruses. Microfluidic technology provides an ideal platform for performing virus diagnostic tests, including sample preparation, immunoassays, and nucleic acid-based assays. Among these techniques, nucleic acid isothermal amplification methods have been widely integrated with microfluidic platforms for RNA virus detection owing to their simplicity, sensitivity, selectivity, and short analysis time. This review summarizes some common isothermal amplification methods for RNA viruses. It also describes commercialized devices and kits that use isothermal amplification techniques for SARS-CoV-2 detection. Furthermore, the most recent applications of isothermal amplification-based microfluidic platforms for RNA virus detection are discussed in this article.

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Section: Introductionmentioning

confidence: 99%

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Ngoc,

Lee

2024

Biosensors

View full text Add to dashboard Cite

show abstract

“…Isothermal amplification techniques can be categorized based on their reaction kinetics into linear amplification, cascade amplification, and exponential amplification. The latter category includes loop-mediated isothermal amplification (LAMP), 12 recombinase polymerase amplification (RPA), 13 helicase-dependent amplification (HDA), 14 nucleic acid sequence-based amplification (NASBA), 15,16 and exponential amplification reaction (EXPAR). 17,18 EXPAR technology utilizes a single-stranded trigger probe (Trigger) X and a single-stranded functional template (Functional template) X′–X′, where X′ is complementary to Probe X.…”

Section: Introductionmentioning

confidence: 99%