Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve

Abstract

Significant progress has been made for tissue imaging of proteins using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS). These advancements now facilitate mapping of a wide range of proteins, peptides, and post-translational modifications in a wide variety of tissues; however, the use of MALDI IMS to detect proteins from cardiac tissue is limited. This review discusses the most recent advances in protein imaging and demonstrates application to cardiac tissue, including the heart valve. Protein imaging by MALDI IMS allows multiplexed histological mapping of proteins and protein components that are inaccessible by antibodies and should be considered an important tool for basic and clinical cardiovascular research.

Metabolomics technology and bioinformatics for precision medicine

“…The mass spectrometry imaging (MSI) can provide such an information. MSI can be performed using either vacuum ionization technique, such as vacuum matrix-assisted laser desorption/ionization (MALDI) [159][160][161][162][163][164], or ambient ionization method, such as atmospheric pressure MALDI (AP-MALDI) [165,166], desorption electrospray ionization [167][168][169][170] and matrixassisted laser desorption electrospray ionization [171,172]. Most often MSI is performed using HRMS.…”

“…Most often MSI is performed using HRMS. MSI has been successfully applied for imaging of various human and animal tissues, such as brain [162,[173][174][175][176], heart [159][160][161]177], liver [178], kidney [179], skin [180], breast [181][182][183][184][185] and lens [163,164]. It has also been used to study clinical samples from cancer patients [181,182,[184][185][186].…”

Novel Techniques in Histologic Research: Morphometry and Mass Spectrometry Imaging

“…MSI is a promising technique for understanding the pathogenesis of lethal acute myocardial infarction (AMI). MSI of cardiac tissue was recently reported by several groups including these authors [21,22].…”

MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section

“…Spraying a thin molecular layer of trypsin onto tissue sections allows imaging of trypsin produced peptides analogous to solution proteomic workflows, with additional information on localization of the proteomes within the tissue architecture [7]. Robust MALDI IMS workflows using the enzyme PNGase F to release N-glycans from thin tissues sections have been developed [6] and are now routinely used to examine distribution of N-glycosylation related to the pathology of disease [8–10].…”