Advances in microfluidic cell separation and manipulation

Jackson, Emily; Lu, Hang

doi:10.1016/j.coche.2013.10.001

Cited by 53 publications

(30 citation statements)

References 80 publications

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“…That is, we look for solutions whereż 1 =ż 2 = constant andα 1 =α 2 = 0. To obtain the initial conditions that lead to this behavior, it is convenient to rewrite the equations of motion (7,9) in terms of the reduced coordinate z 1 − z 2 which we set to z 1 − z 2 = β = ce iθ (see inset of Figure 1(a)). To this end, one getṡ…”

Section: Pursuit and Synchronizationmentioning

confidence: 99%

Pursuit and Synchronization in Hydrodynamic Dipoles

Kanso

Tsang

2015

J Nonlinear Sci

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We study theoretically the behavior of a class of hydrodynamic dipoles. This study is motivated by recent experiments on synthetic and biological swimmers in microfluidic Hele-Shaw type geometries. Under such confinement, a swimmer's hydrodynamic signature is that of a potential source dipole, and the long-range interactions among swimmers are obtained from the superposition of dipole singularities. Here, we recall the equations governing the positions and orientations of interacting asymmetric swimmers in doubly-periodic domains, and focus on the dynamics of swimmer pairs. We obtain two families of 'relative equilibria'-type solutions that correspond to pursuit and synchronization of the two swimmers, respectively. Interestingly, the pursuit mode is stable for large tail swimmers whereas the synchronization mode is stable for large head swimmers. These results have profound implications on the collective behavior reported in several recent studies on populations of confined microswimmers.

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Section: Pursuit and Synchronizationmentioning

confidence: 99%

Pursuit and Synchronization in Hydrodynamic Dipoles

Kanso

Tsang

2015

J Nonlinear Sci

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“…A major motivation to shift toward applying microfluidics for separation purposes is to minimize the size, cost, and complexity of technologies by reducing the sample size, performing the reactions faster, and conducting multiple assays in small footprints simultaneously (Salieb-Beugelaar 2010, Jackson 2013). …”

Section: - Ev Isolation Techniques An Essential Preparatory Step Inmentioning

confidence: 99%

“…(D) Magnetic-based technique: an applied magnetic field is used to deflect and focus cells labeled with magnetic particles (green). Adapted with permission from ref (Jackson 2013), Copyright 2013, Elsevier.…”

Section: Figurementioning

confidence: 99%

Microfluidic approaches for isolation, detection, and characterization of extracellular vesicles: Current status and future directions

Gholizadeh

Draz

Zarghooni

et al. 2017

Biosensors and Bioelectronics

178

113

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Extracellular vesicles (EVs) are cell-derived vesicles present in body fluids that play an essential role in various cellular processes, such as intercellular communication, inflammation, cellular homeostasis, survival, transport, and regeneration. Their isolation and analysis from body fluids have a great clinical potential to provide information on a variety of disease states such as cancer, cardiovascular complication and inflammatory disorders. Despite increasing scientific and clinical interest in this field, at the time of writing there are still no standardized procedures available for the purification, detection, and characterization of EVs. Advances in microfluidics allow for chemical sampling with increasingly high spatial resolution and under precise manipulation down to single molecule level. In this review, our objective is to give a brief overview on the working principle and examples of the isolation and detection methods with the potential to be used for extracellular vesicles. This review will also highlight the integrated on-chip systems for isolation and characterization of EVs.

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“…Microfluidic devices with the capability to detect, sort, and separate specific cells are considered strong candidates. Recent developments in the field of microfluidics have led to considerable advancements in microscale separation systems . Microfluidic separation devices need only small sample and reagent volumes, which have the advantages of reduced cost and analysis time .…”

Section: Introductionmentioning

confidence: 99%

“…to considerable advancements in microscale separation systems [5][6][7][8]. Microfluidic separation devices need only small sample and reagent volumes, which have the advantages of reduced cost and analysis time [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

2017

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We describe the design, microfabrication, and testing of a microfluidic device for the separation of cancer cells based on dielectrophoresis. Cancer cells, specifically green fluorescent protein-labeled MDA-MB-231, are successfully separated from a heterogeneous mixture of the same and normal blood cells. MDA-MB-231 cancer cells are separated with an accuracy that enables precise detection and counting of circulating tumor cells present among normal blood cells. The separation is performed using a set of planar interdigitated transducer electrodes that are deposited on the surface of a glass wafer and slightly protrude into the separation microchannel at one side. The device includes two parts, namely, a glass wafer and polydimethylsiloxane element. The device is fabricated using standard microfabrication techniques. All experiments are conducted with low conductivity sucrose-dextrose isotonic medium. The variation in response between MDA-MB-231 cancer cells and normal cells to a certain band of alternating-current frequencies is used for continuous separation of cells. The fabrication of the microfluidic device, preparation of cells and medium, and flow conditions are detailed. The proposed microdevice can be used to detect and separate malignant cells from heterogeneous mixture of cells for the purpose of early screening for cancer.

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Advances in microfluidic cell separation and manipulation

Cited by 53 publications

References 80 publications

Pursuit and Synchronization in Hydrodynamic Dipoles

Pursuit and Synchronization in Hydrodynamic Dipoles

Microfluidic approaches for isolation, detection, and characterization of extracellular vesicles: Current status and future directions

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

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