Advances in Nerve Guidance Conduit-Based Therapeutics for Peripheral Nerve Repair

Lackington, William A.; Ryan, Alan J.; O’Brien, Fergal J.

doi:10.1021/acsbiomaterials.6b00500

Cited by 103 publications

(92 citation statements)

References 165 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nerve-guide-conduits or nerve-guidance-channels (NGCs) are 3D constructs for whole nerve therapies in vivo (Anderson et al, 2017;Lackington et al, 2017). They are currently used as implants for neural repair in humans.…”

Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

View full text Add to dashboard Cite

As they differentiate from neuroblasts, nascent neurons become highly polarized and elongate. Neurons extend and elaborate fine and fragile cellular extensions that form circuits enabling long-distance communication and signal integration within the body. While other organ systems are developing, projections of differentiating neurons find paths to distant targets. Subsequent post-developmental neuronal damage is catastrophic because the cues for reinnervation are no longer active. Advances in biomaterials are enabling fabrication of micro-environments that encourage neuronal regrowth and restoration of function by recreating these developmental cues. This mini-review considers new materials that employ topographical, chemical, electrical, and/or mechanical cues for use in neuronal repair. Manipulating and integrating these elements in different combinations will generate new technologies to enhance neural repair.

show abstract

Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

View full text Add to dashboard Cite

show abstract

“…In addition, microscale topographical cues that mimic the peripheral nerve bundle can significantly affect the microcellular environment such as alignment, extension of axons, and dendrites. [7,9,[12][13][14][15] In our previous studies, we have performed various experiments using topographical cues to mimic target tissues appropriately, and we confirmed that groove-ridge pattern can significantly improve cell migration and differentiation. [16][17][18][19] We have found that topographical substrate could enhance neurite outgrowth and alignment by using various sizes of poly(lactic-co-glycolic acid) (PLGA) topographical substrates.…”

Section: Introductionmentioning

confidence: 73%

“…Among FDA‐approved NGCs, filled and hollowed NGCs are already commercially available, such as NeuraGen 3D (Integra LifeScience) and Neurolac (Polyganics Inc.). These NGCs can induce different results of nerve regeneration depending on animal model, nerve gap, and defect size . Some research studies have reported that NGCs filled with inner filler in lumen can serve as guidance channel to enhance axonal regeneration compared to hollow NGCs .…”

Section: Discussionmentioning

confidence: 99%

“…These NGCs can induce different results of nerve regeneration depending on animal model, nerve gap, and defect size. [9,31] Some research studies have reported that NGCs filled with inner filler in lumen can serve as guidance channel to enhance axonal regeneration compared to hollow NGCs. [32] However, another researcher has mentioned that the inner filler may only be beneficial when it is impermeable to infiltrate fibrotic tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Several advanced techniques, such as nanoimprint lithography, solvent casting, particulate leaching, gas forming, electrospinning, and 3D printing techniques, have been applied to fabricate micro-or nanoscale tissue-engineered nerve guidance conduits (NGCs) with various degrees of success. [7,9,10] Using those techniques, many researchers have fabricated various kinds of NGCs that provide high permeability on the outer surface or have aligned electrospun fibers on the inner surface on conduit. [7,11] However, for nerve regeneration, these two individual characteristics might need to be modified simultaneously for NGC in order to provide permeability for the inflow of oxygen, nutrients, and exogenous growth factors while regulating cell behavior and tissue progression by topographical cue.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradable Nerve Guidance Conduit with Microporous and Micropatterned Poly(lactic‐co‐glycolic acid)‐Accelerated Sciatic Nerve Regeneration

Kim

Lee

Jeon

et al. 2018

Macromolecular Bioscience

View full text Add to dashboard Cite

An innovative technique combining capillary force lithography and phase separation method in one step is applied to fabricate artificial nerve guidance conduit (NGC) for peripheral nerve regeneration. Biodegradable porous, patterned NGC (PP‐NGC) using poly(lactic‐co‐glycolic acid) is fabricated. It has micro‐grooves and microporosity on the inner surface to promote axonal outgrowth and to enhance permeability for nutrient exchange. In this study, it is confirmed that the inner surface of micro‐grooves can modulate neurite orientation and length of mouse neural stem cell compared to porous flat NGC (PF‐NGC) in vitro. Coating with 3,4‐dihydroxy‐l‐phenylalanine (DOPA) facilitates the hydrophilic inner surface of PF‐ and PP‐NGCs via bioinspired catechol chemistry. For in vivo study, PF‐NGC and PP‐NGC coated with or without DOPA are implanted in the 10 mm sciatic nerve defect margins between proximal and distal nerves in rats. Especially, PP‐NGC coated with DOPA shows higher sciatic function index score, onset‐to‐peak amplitude, and muscle fiber diameter compared to other groups. The proposed hybrid‐structured NGC not only can serve as a design for functional NGC without growth factor but also can be used in clinical application for peripheral nerve regeneration.

show abstract

Electrically Conductive Hydrogel Nerve Guidance Conduits for Peripheral Nerve Regeneration

Park

Jeon

Kim

et al. 2020

Adv Funct Materials

168

111

View full text Add to dashboard Cite

Peripheral nerve injuries are serious conditions, and surgical treatment has critical limitations. Therefore, nerve guidance conduits (NGCs) are proposed as an alternative. In this study, multifunctional NGCs are fabricated for the regeneration of injured peripheral nerves. Graphene oxide (GO) and gelatin-methacrylate (GelMA) are polymerized and chemically reduced to form reduced (GO/GelMA) (r(GO/GelMA)). The prepared materials present good electrical conductivity, flexibility, mechanical stability, and permeability, which are suitable for use as NGCs. In vitro studies show 2.1-and 1.4-fold promotion of neuritogenesis of PC12 neuronal cells on r(GO/GelMA) compared to GelMA and unreduced GO/GelMA, respectively. Animal studies using a rat sciatic nerve injury model with a 10 mm gap between the proximal and distal regions of the defect reveal that r(GO/GelMA) NGCs significantly enhance peripheral nerve regeneration, indicated by improved muscle weight increase, electro-conduction velocity, and sciatic nerve function index. Specifically, r(GO/GelMA) NGCs are utilized to potentiate regrowth with myelination in rat sciatic nerves followed by histological, immunohistological, and morphometrical analyses. This study successfully shows the feasibility of electrically conductive hydrogel NGCs as functional conduits for improved nerve regeneration in a preclinical study, where these NGCs can not only mimic nerve tissues but also strongly promote nerve regeneration.

show abstract

Advances in Nerve Guidance Conduit-Based Therapeutics for Peripheral Nerve Repair

Abstract: Peripheral nerve injuries have high incidence rates, limited treatment options and poor clinical outcomes, rendering a significant socioeconomic burden. For effective peripheral nerve repair, the gap or site of injury must be structurally bridged to promote correct

Cited by 103 publications

References 165 publications

Biomaterials for Enhancing Neuronal Repair

Biomaterials for Enhancing Neuronal Repair

Biodegradable Nerve Guidance Conduit with Microporous and Micropatterned Poly(lactic‐co‐glycolic acid)‐Accelerated Sciatic Nerve Regeneration

Electrically Conductive Hydrogel Nerve Guidance Conduits for Peripheral Nerve Regeneration

Contact Info

Product

Resources

About