Advances in phage display technology for drug discovery

Omidfar, Kobra; Daneshpour, Maryam

doi:10.1517/17460441.2015.1037738

Cited by 106 publications

(58 citation statements)

References 166 publications

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“…Here we demonstrate the existence of a peptide-dependent mechanism capable of moving particles and/or molecules across an intact un-perforated infected TM and into the ME. The process was uncovered using phage display, an efficient, versatile and rapid means by which to identify compounds with desired biochemical and biological characteristics for an array of targets 24 26 29 38 39 . The transport was specific because a synthetic peptide blocked interaction between the phage and the TM.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a Biological Mechanism of Active Transport through the Tympanic Membrane to the Middle Ear

Kurabi

Pak

Bernhardt

et al. 2016

Sci Rep

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Otitis media (OM) is a common pediatric disease for which systemic antibiotics are often prescribed. While local treatment would avoid the systemic treatment side-effects, the tympanic membrane (TM) represents an impenetrable barrier unless surgically breached. We hypothesized that the TM might harbor innate biological mechanisms that could mediate trans-TM transport. We used two M13-bacteriophage display biopanning strategies to search for mediators of trans-TM transport. First, aliquots of linear phage library displaying 1010th 12mer peptides were applied on the TM of rats with active bacterial OM. The middle ear (ME) contents were then harvested, amplified and the preparation re-applied for additional rounds. Second, the same naïve library was sequentially screened for phage exhibiting TM binding, internalization and then transit. Results revealed a novel set of peptides that transit across the TM to the ME in a time and temperature dependent manner. The peptides with highest transport capacities shared sequence similarities. Historically, the TM was viewed as an impermeable barrier. However, our studies reveal that it is possible to translocate peptide-linked small particles across the TM. This is the first comprehensive biopanning for the isolation of TM transiting peptidic ligands. The identified mechanism offers a new drug delivery platform into the ME.

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Section: Discussionmentioning

confidence: 99%

Discovery of a Biological Mechanism of Active Transport through the Tympanic Membrane to the Middle Ear

Kurabi

Pak

Bernhardt

et al. 2016

Sci Rep

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“…Phage display was developed in 1985 [9] and is still the most commonly used display method [10]. However, display can also be done in yeast [11] or bacteria [12].…”

Section: Peptides Derived Through High-throughput Methodsmentioning

confidence: 99%

Peptides and peptidomimetics as regulators of protein–protein interactions

Cunningham

Qvit

Mochly‐Rosen

2017

Current Opinion in Structural Biology

134

107

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Protein-protein interactions are essential for almost all intracellular and extracellular biological processes. Regulation of protein-protein interactions is one strategy to regulate cell fate in a highly selective manner. Specifically, peptides are ideal candidates for inhibition of protein-protein interactions because they can mimic a protein surface to effectively compete for binding. Additionally, peptides are synthetically accessible and can be stabilized by chemical modifications. In this review, we survey screening and rational design methods for identifying peptides to inhibit protein-protein interactions, as well as methods for stabilizing peptides to effectively mimic protein surfaces. In addition, we discuss recent applications of peptides to regulate protein-protein interactions for both basic research and therapeutic purposes.

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“…The list is even broader and even more applications are expected to arise as the methodology evolves. [58][59][60][61][62][63][64][65][66][67]…”

Section: Why Use Recombinant Phages?mentioning

confidence: 99%

Genetically modified bacteriophages

et al. 2016

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Phages or bacteriophages, viruses that infect and replicate inside bacteria, are the most abundant microorganisms on earth. The realization that antibiotic resistance poses a substantial risk to the world's health and global economy is revitalizing phage therapy as a potential solution. The increasing ease by which phage genomes can be modified, owing to the influx of new technologies, has led to an expansion of their natural capabilities, and a reduced dependence on phage isolation from environmental sources. This review will discuss the way synthetic biology has accelerated the construction of genetically modified phages and will describe the wide range of their applications. It will further provide insight into the societal and economic benefits that derive from the use of recombinant phages in various sectors, from health to biodetection, biocontrol and the food industry.

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Advances in phage display technology for drug discovery

Cited by 106 publications

References 166 publications

Discovery of a Biological Mechanism of Active Transport through the Tympanic Membrane to the Middle Ear

Discovery of a Biological Mechanism of Active Transport through the Tympanic Membrane to the Middle Ear

Peptides and peptidomimetics as regulators of protein–protein interactions

Genetically modified bacteriophages

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