Advances in separation and identification of biologically important milk proteins and peptides

Št́astná, Miroslava

doi:10.1002/elps.202300084

Cited by 9 publications

(7 citation statements)

References 156 publications

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“…The cargo of milk exosomes may include molecules such as RNA, proteins, and lipids, and milk exosomes. They may include a wide variety of proteins that perform functions such as cell signaling, cell adhesion, and regulation of immune responses [45,48]. The membranes of milk exosomes may vary depending on the type of exosome-producing cells, individual differences, and physiological conditions.…”

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

“…Transported growth factors and signaling proteins can reach recipient cells and regulate physiological processes such as cell division, cell survival, and tissue regeneration [48]. Growth factors affect cell survival, growth, division, and differentiation.…”

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

“…Growth factors affect cell survival, growth, division, and differentiation. Milk exosomes carry growth factors and deliver them to the recipient cells [48]. Growth factors bind to cell surface receptors and initiate signal transduction, which activates intracellular signaling pathways and induces physiological changes in the cells [48].…”

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

“…Milk exosomes carry growth factors and deliver them to the recipient cells [48]. Growth factors bind to cell surface receptors and initiate signal transduction, which activates intracellular signaling pathways and induces physiological changes in the cells [48]. Growth factors contribute to various physiological functions, including cell growth, tissue regeneration, immune response regulation, and angiogenesis [48].…”

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

“…Growth factors bind to cell surface receptors and initiate signal transduction, which activates intracellular signaling pathways and induces physiological changes in the cells [48]. Growth factors contribute to various physiological functions, including cell growth, tissue regeneration, immune response regulation, and angiogenesis [48]. Signaling proteins are involved in both intracellular and intercellular signal transduction [47,48].…”

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

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Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Kim,

Lee

et al. 2023

Nutrients

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Milk is a nutrient-rich food source, and among the various milks, breast milk is a nutrient source provided by mothers to newborns in many mammals. Exosomes are nano-sized membranous extracellular vesicles that play important roles in cell-to-cell communication. Exosomes originate from endogenous synthesis and dietary sources such as milk. Discovered through electron microscopy as floating vesicles, the existence of exosomes in human milk was confirmed owing to a density between 1.10 and 1.18 g/mL in a sucrose gradient corresponding to the known density of exosomes and detection of MHC classes I and II, CD63, CD81, and CD86 on the vesicles. To date, milk exosomes have been used for treating many diseases, including cancers, and are widely proposed as promising carriers for the delivery of chemotherapeutic agents. However, few studies on milk exosomes focus on geriatric health, especially sarcopenia and osteoporosis related to bone and muscle. Therefore, the present study focused on milk exosomes and their cargoes, which are potential candidates for dietary supplements, and when combined with drugs, they can be effective in treating musculoskeletal diseases. In this review, we introduce the basic concepts, including the definition, various sources, and cargoes of milk exosomes, and exosome isolation and characterization methods. Additionally, we review recent literature on the musculoskeletal system and milk exosomes. Since inflammation and oxidative stress underly musculoskeletal disorders, studies reporting the antioxidant and anti-inflammatory properties of milk exosomes are also summarized. Finally, the therapeutic potential of milk exosomes in targeting muscle and bone health is proposed.

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Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

Section: Cargo Of Milk Exosomesmentioning

confidence: 99%

See 3 more Smart Citations

Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Kim,

Lee

et al. 2023

Nutrients

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Capillary Electrophoresis in Food Analysis

Castro‐Puyana,

Bernardo‐Bermejo,

Abad‐Gil

et al. 2024

Encyclopedia of Analytical Chemistry

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The development and application of faster and more powerful analytical methodologies in Food Science is essential to face the demands from regulatory agencies, official laboratories, and consumers. In this field, capillary electrophoresis (CE) is nowadays a well‐established separation technique for food analysis that possesses exceptional properties related to high analysis speed and separation efficiencies combined with the small samples and reagent requirements. Moreover, it is a very versatile technique since different CE modes can be employed for a broad range of applications and separation challenges. In fact, CE should be considered as the first option when dealing with highly polar, charged, or chiral analytes. In the past two decades, a high amount of research work has been published, demonstrating the high potential of CE to analyze different food components in a broad variety of food matrices. The aim of this article is to provide an overview of the use of CE for food analysis including a summary of the characteristics of the CE modes and the main detectors mostly employed in this field. Besides, a wide view of the latest applications of CE in food analysis (2021–2023) is also provided, demonstrating the exceptional possibilities of this analytical technique.

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Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview

Kartsova,

Maliushevska

2024

J of Separation Science

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This review provides an overview of recent works focusing on the determination of amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection and ultraviolet (UV) detection, which is the most widespread detection in capillary electromigration techniques, without pre‐capillary derivatization. Available options for the UV detection of these analytes, such as indirect detection, complexation with transition metal ions, and in‐capillary derivatization are described. Developments in the field of direct detection of UV‐absorbing AAs and peptides as well as progress in chiral separation are described. A separate section is dedicated to using on‐line sample preconcentration methods combined with capillary electrophoresis‐UV.

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Advances in separation and identification of biologically important milk proteins and peptides

Cited by 9 publications

References 156 publications

Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Capillary Electrophoresis in Food Analysis

Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview

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