Advances in the Bacteriophage-Based Precise Identification and Magnetic Relaxation Switch Sensor for Rapid Detection of Foodborne Pathogens

Abstract

The development of novel and highly specific technologies for the rapid and sensitive detection of foodborne pathogens is very important for disease prevention and control. Bacteriophages can recognize viable and unviable bacteria, replacing antibodies as the recognition element in the immune response, which are currently being widely developed in novel precise identification biosensors. Magnetic relaxation switch sensors based on the magnetic relaxation signal has been used to construct a variety of background-free novel biosensors in recent years, which can realize rapid detection of foodborne pathogens. This chapter will mainly introduce the latest developments and future prospects of bacteriophages in the field of accurate identifications for foodborne pathogens. At the same time, it will introduce the research progress and development direction of novel magnetic relaxation switch sensors for detecting foodborne pathogens.

Multimodal Biosensing of Foodborne Pathogens

“…Bacteriophages, which can recognize viable and non-viable bacteria, are used as the detection element in these biosensors, replacing antibodies. The nanocomposites in these biosensors can enhance signal transduction and improve detection sensitivity [6]. Microfluidic chip biosensors have also been developed for the identification of foodborne pathogens using nanocomposites.…”

“…Bacteriophages, which can distinguish between viable and non-viable bacteria, have been used as the recognition components in these biosensors, replacing antibodies. The combination of bacteriophages and magnetic relaxation switch sensors enables precise identification of foodborne pathogens [6]. A magnetic biosensor was developed for sensing Salmonella using curcumin to report the signal as well as click chemistry to amplify the signal.…”

“…biosensor-based techniques, multimodal biosensing can enhance the sensitivity, specificity, and rapidity of pathogen recognition [4][5][6][7]. As an example, the combination of nucleic acid-based methods with biosensor-based methods can provide rapid and accurate detection by targeting specific genetic sequences of pathogens and detecting their presence using highly sensitive biosensors [4].…”

Multimodal Biosensing of Foodborne Pathogens

“…Another example is using a dual-readout biosensor for detecting nucleic acids and non-nucleic acids utilizing a CRISPR-ALP tandem analysis, demonstrating the versatility and efficiency of multimodal biosensors in detecting single targets with different characteristics [ 5 , 7 , 8 ]. Furthermore, by integrating different sensing techniques, such as immunological approaches, nucleic acid-based procedures, and biosensor-based techniques, multimodal biosensing can enhance the sensitivity, specificity, and rapidity of the detection of pathogens [ 9 , 10 , 11 , 12 ]. As an example, the combination of nucleic acid-based methods with biosensor-based methods provides rapid and accurate detection by targeting specific genetic sequences of pathogens and detecting their presence using highly sensitive biosensors [ 4 ].…”