Advances in the metabolic engineering of
 Saccharomyces cerevisiae
 and
 Yarrowia lipolytica
 for the production of
 β
 -carotene

Guo, Qi; Peng, Qianqian; Li, Ya-Wen; Yan, Fang; Wang, Yuetong; Ye, Chao; Shi, Tian‐Qiong

doi:10.1080/07388551.2023.2166809

Cited by 10 publications

(6 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, this study represents the first report of coupling erythritol production to β-carotene synthesis. Although the production levels may be slightly lower compared to other engineered strains that have undergone complex multistep optimization, 11 the β-carotene obtained through coproduction in this study can be considered cost-effective, and the recovered cells after erythritol production may have commercial value for βcarotene extraction.…”

Section: Batch Process For Erythritol and β-Carotenementioning

confidence: 99%

“…Y. lipolytica has garnered much interest for the heterologous synthesis of β-carotene . Recently, Ma et al.…”

Section: Introductionmentioning

confidence: 99%

“…10 Y. lipolytica has garnered much interest for the heterologous synthesis of β-carotene. 11 Recently, Ma et al used two different strategies to overcome the inhibition of the lycopene substrate, resulting in the production of 39.5 g/L β-carotene at a productivity of 0.165 g/(L•h) in fed-batch fermentation. 12 Although significant progress has been made in the production of β-carotene using yeast platforms and low-cost substrates, the low levels of β-carotene yield continue to maintain prices high.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Zhang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

While the expansion of the erythritol production industry has resulted in unprecedented production of yeast cells, it also suffers from a lack of effective utilization. β-Carotene is a value-added compound that can be synthesized by engineered Yarrowia lipolytica. Here, we first evaluated the production performance of erythritol-producing yeast strains under two different morphologies and then successfully constructed a chassis with yeast-like morphology by deleting Mhy1 and Cla4 genes. Subsequently, β-carotene synthesis pathway genes, CarRA and CarB from Blakeslea trispora, were introduced to construct the βcarotene and erythritol coproducing Y. lipolytica strain ylmcc. The rate-limiting genes GGS1 and tHMG1 were overexpressed to increase the β-carotene yield by 45.32-fold compared with the strain ylmcc. However, increased β-carotene accumulation led to prolonged fermentation time; therefore, transporter engineering through overexpression of YTH1 and YTH3 genes was used to alleviate fermentation delays. Using batch fermentation in a 3 L bioreactor, this engineered Y. lipolytica strain produced erythritol with production, yield, and productivity values of 171 g/L, 0.56 g/g glucose, and 2.38 g/(L•h), respectively, with a concomitant βcarotene yield of 47.36 ± 0.06 mg/g DCW. The approach presented here improves the value of erythritol-producing cells and offers a low-cost technique to obtain hydrophobic terpenoids.

show abstract

Section: Batch Process For Erythritol and β-Carotenementioning

confidence: 99%

“…Y. lipolytica has garnered much interest for the heterologous synthesis of β-carotene . Recently, Ma et al.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Zhang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…One of the most significant carotenoids is β-carotene. β-carotene, commonly known as pro-vitamin A, is a yellowish carotenoid pigment with antioxidant and disease-fighting potential [113,114]. P. blakesleeanus, M. circinelloides [115], and B. trispora are examples of mucorales fungi that contain yellowish-carotene, one of nature's most abundant carotenes.…”

Section: β-Carotenementioning

confidence: 99%

“…Yeasts such as Saccharomyces cerevisiae and Yarrowia lipolytica have garnered the most interest due to their robustness for large-scale fermentation, security, and sophisticated genetic manipulation tools. Carotenoids that use the native mevalonate pathway also get an advantage [114].…”

Section: β-Carotenementioning

confidence: 99%

Fungal Pigments: Carotenoids, Riboflavin, and Polyketides with Diverse Applications

Toma

Rahman

et al. 2023

JoF

View full text Add to dashboard Cite

Natural pigments and colorants have seen a substantial increase in use over the last few decades due to their eco-friendly and safe properties. Currently, customer preferences for more natural products are driving the substitution of natural pigments for synthetic colorants. Filamentous fungi, particularly ascomycetous fungi (Monascus, Fusarium, Penicillium, and Aspergillus), have been shown to produce secondary metabolites containing a wide variety of pigments, including β-carotene, melanins, azaphilones, quinones, flavins, ankaflavin, monascin, anthraquinone, and naphthoquinone. These pigments produce a variety of colors and tints, including yellow, orange, red, green, purple, brown, and blue. Additionally, these pigments have a broad spectrum of pharmacological activities, including immunomodulatory, anticancer, antioxidant, antibacterial, and antiproliferative activities. This review provides an in-depth overview of fungi gathered from diverse sources and lists several probable fungi capable of producing a variety of color hues. The second section discusses how to classify coloring compounds according to their chemical structure, characteristics, biosynthetic processes, application, and present state. Once again, we investigate the possibility of employing fungal polyketide pigments as food coloring, as well as the toxicity and carcinogenicity of particular pigments. This review explores how advanced technologies such as metabolic engineering and nanotechnology can be employed to overcome obstacles associated with the manufacture of mycotoxin-free, food-grade fungal pigments.

show abstract

A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production

Uçar,

Demirgül,

Şimşek

et al. 2024

Biotechnol Bioproc E

View full text Add to dashboard Cite

Advances in the metabolic engineering of Saccharomyces cerevisiae and Yarrowia lipolytica for the production of β -carotene

Cited by 10 publications

References 103 publications

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Concomitant Production of Erythritol and β-Carotene by Engineered Yarrowia lipolytica

Fungal Pigments: Carotenoids, Riboflavin, and Polyketides with Diverse Applications

A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production

Contact Info

Product

Resources

About